Methods recombinant protein production (29 resultados)

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Paperback. Condición: Brand New. 180 pages. 8.66x5.91x0.41 inches. In Stock.

Condición: New. pp. 260.

Condición: New. 2016. Paperback. . . . . .

Condición: New. In English.

Condición: New.

Condición: New. 2016. Paperback. . . . . . Books ship from the US and Ireland.

Condición: New.

Condición: New. In English.

Condición: New.

Condición: New. In English.

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Hardcover. Condición: Brand New. 1st edition. 248 pages. 10.25x7.25x1.00 inches. In Stock.

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Condición: New. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in Methods in Microbiology serials Updated release includes the latest information on Methods in Recom.

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Buch. Condición: Neu. Neuware - Methods in Microbiology serial highlights new advances in the field with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

Taschenbuch. Condición: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Scientific methods of enhancing gene expression including genomics, applied molecular biology, metabolic engineering, and combinatorial biosynthesis have been sufficiently developed to make a major impact on recombinant protein production. However, it appears that the combination of trial-and-error experimentation and molecular biology are not sufficient to design an optimum strategy to reach the goal. This book provides a thorough coverage of theoretical and design aspects of two engineering methodologies to overcome this shortcoming. Special emphasis was placed on the effect of the fermentation medium components of A.niger on a recombinant protein production. Statistically based experimental designs in a sequential manner were introduced as powerful tools and applied for medium optimization. A detailed metabolic network comprising intracellular compartments, amino acid catabolism and experimental flux measurements of the key metabolites should be developed and used for optimization purposes. The proposed strategy should be useful to professionals in the field of biotechnology who seek to understand the response of microorganisms to environmental stimuli.…

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Condición: new. Questo è un articolo print on demand.

Condición: New. Print on Demand pp. 260.

Condición: New. PRINT ON DEMAND pp. 260.

Hardcover. Condición: Brand New. 210 pages. 9.00x6.00x0.55 inches. In Stock. This item is printed on demand.

Taschenbuch. Condición: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.…

Buch. Condición: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.…