9781489943309 - Protein Lipidation Protocols: 116 (Methods in Molecular Biology) (13 resultados)

Condición: New. In.

Condición: New. pp. 252.

Condición: New. Editor(s): Gelb, Michael H. Series: Methods in Molecular Biology. Num Pages: 240 pages, 35 black & white illustrations, biography. BIC Classification: PSB. Category: (P) Professional & Vocational. Dimension: 229 x 152 x 13. Weight in Grams: 374. . 2013. Softcover reprint of the original 1st ed. 1999. Paperback. . . . .

Taschenbuch. Condición: Neu. Protein Lipidation Protocols | Michael H. Gelb | Taschenbuch | x | Englisch | 2013 | Humana | EAN 9781489943309 | Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu.

Paperback. Condición: Brand New. 250 pages. 9.10x6.00x0.60 inches. In Stock.

Condición: New. Editor(s): Gelb, Michael H. Series: Methods in Molecular Biology. Num Pages: 240 pages, 35 black & white illustrations, biography. BIC Classification: PSB. Category: (P) Professional & Vocational. Dimension: 229 x 152 x 13. Weight in Grams: 374. . 2013. Softcover reprint of the original 1st ed. 1999. Paperback. . . . . Books ship from the US and Ireland.

Taschenbuch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -In Protein Lipidation Protocols, Michael Gelb brings together a collection of readily reproducible techniques for studying protein lipidation, the covalent attachment of lipids to proteins. These cutting-edge methods-many never published before in a 'hands-on' format-deal with glycosyl phosphatidylinositol (GPI)-containing compounds, protein fatty acylation, and protein prenylation. Included are novel techniques for determining the chemical structure of GPI-anchors, for radiolabeling the prenyl groups of protein in eukaryotic cells, a tool for developing inhibitors of the protein farnesyltransferase, and for an exciting lysosomal enzyme that cleaves fatty acyl groups from proteins, the first fatty acylase discovered. Protein Lipidation Protocols offers biochemists, cell and molecular biologists, medicinal chemists, and pharmaceutical researchers state-of-the-art tools for understanding the complex biochemistry of protein lipidation, as well as catalyzing the development of many important new biopharmaceuticals, including anticancer drugs. 252 pp. Englisch.

Condición: new. Questo è un articolo print on demand.

Condición: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. In Vitro Analysis of GPI Biosynthesis in Mammalian Cells, Victoria L. Stevens. Selection of Mammalian Cell Mutants in GPI Biosynthesis, Victoria L. Stevens. Analysis of the Cell-Surface Distribution of GPI-Anchored Proteins, Satyajit Mayor. Imaging Fluoresc.

Condición: New. Print on Demand pp. 252 23:B&W 6 x 9 in or 229 x 152 mm Perfect Bound on White w/Gloss Lam.

Taschenbuch. Condición: Neu. This item is printed on demand - Print on Demand Titel. Neuware -In Vitro Analysis of GPI Biosynthesis in Mammalian Cells.- Selection of Mammalian Cell Mutants in GPI Biosynthesis.- Analysis of the Cell-Surface Distribution of GPI-Anchored Proteins.- Imaging Fluorescence Resonance Energy Transfer as Probe of Membrane Organization and Molecular Associations of GPI-Anchored Proteins.- Purification of Caveolae-Derived Membrane Microdomains Containing Lipid-Anchored Signaling Molecules, Such as GPI-Anchored Proteins, H-Ras, Src-Family Tyrosine Kinases, eNOS, and G-Protein -, -, and -Subunits.- Analysis of Lipids in Caveolae.- Analysis of the Carbohydrate Components of Glycosylphosphatidylinositol Structures Using Fluorescent Labeling.- Analysis of the Lipid Moiety of GPI Anchor in the Yeast Saccharomyces cerevisiae.- Rapid Identification of Cysteine-Linked Isoprenyl Groups by Metabolic Labeling with [3H]Farnesol and [3H]Geranylgeraniol.- Incorporation of Radiolabeled Prenyl Alcohols and Their Analogs into Mammalian Cell Proteins.- Reconstitution of Yeast Farnesyltransferase from Individually Purified Subunits.- Probing the Role of H-Ras Lipidation for Signaling Functions in Xenopus laevis Oocytes.- Fluorescence Measurement of Lipid-Binding Affinity and Interbilayer Transfer of Bimane-Labeled Lipidated Peptides.- Determination of the Kinetics of Intervesicle Transfer and Transbilayer Diffusion of Bimane-Labeled Lipidated Peptides.- Preparation and Assay of Myristoyl-CoA:Protein N-Myristoyltransferase.- Metabolic Labeling of Protein-Derived Lipid Thioesters in Palmitoyl-Protein Thioesterase-Deficient Cells.- Fatty Acid Analysis of Protein-Derived Lipid Thioesters Isolated from Palmitoyl-Protein Thioesterase-Deficient Cells.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 252 pp. Englisch.

Taschenbuch. Condición: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - In Protein Lipidation Protocols, Michael Gelb brings together a collection of readily reproducible techniques for studying protein lipidation, the covalent attachment of lipids to proteins. These cutting-edge methods-many never published before in a 'hands-on' format-deal with glycosyl phosphatidylinositol (GPI)-containing compounds, protein fatty acylation, and protein prenylation. Included are novel techniques for determining the chemical structure of GPI-anchors, for radiolabeling the prenyl groups of protein in eukaryotic cells, a tool for developing inhibitors of the protein farnesyltransferase, and for an exciting lysosomal enzyme that cleaves fatty acyl groups from proteins, the first fatty acylase discovered. Protein Lipidation Protocols offers biochemists, cell and molecular biologists, medicinal chemists, and pharmaceutical researchers state-of-the-art tools for understanding the complex biochemistry of protein lipidation, as well as catalyzing the development of many important new biopharmaceuticals, including anticancer drugs.

Condición: New. PRINT ON DEMAND pp. 252.