Psol maryna (18 resultados)

Condición: New.

Condición: New.

Condición: New.

Taschenbuch. Condición: Neu. Druck auf Anfrage Neuware - Printed after ordering - Internship Report from the year 2012 in the subject Biology - Neurobiology, grade: 1,3, University of Göttingen (Max Plank Institute for Experimental Medicine / Department of Neurogenetics), language: English, abstract: Tetraspanin2 (Tspan2) is a member of the tetraspan/transmembrane4 superfamily restricted to the nervous system. The abundance of Tspan2 is low in physiological condition but increases greatly in myelin of PLP-deficient mice which may indicate its' compensatory functions. Our experiments show that Tspan2 has no effect on delay of myelination at P14; young and old mice lacking Tspan2 has the same g-ratio as wild type. However, the quantity of unhealthy axons and axonal swellings are higher in aged animals lacking both PLP and Tspan2 than in single PLP knockout. Furthermore,the absence of PLP causes the decrease in the number of axons and rise in axon diameter; lack of Tspan2 alone leads to decrease in quantity of 0.4-0.7 µm diameter axons in 40 weeks old mice. These findings point to a possible auxiliary role of Tspan2 in support of axonal transport and long-term axon preservation in PLP null condition. …

Taschenbuch. Condición: Neu. Druck auf Anfrage Neuware - Printed after ordering - Project Report from the year 2013 in the subject Biology - Neurobiology, grade: 1.0, University of Göttingen, language: English, abstract: Genetic engineering is known as a powerful technique for basic research and clinical applications. Recent progress in development of zinc-finger nucleases (ZFNs), which combine the DNA cleavage ability of Fok1 restriction enzyme with highly specific recognition properties of zinc-finger motifs, allows to improve efficiency and to broaden the field of use of genome editing. Here, we demonstrate our initial results in generating novel tools for Cathepsin D gene knockout in neurons based on ZFNs technology and mediated by adeno-associated virus (AAV) vectors. Pairs of AAV-ZFNs were produced and demonstrated the robust expression of nucleases in neuronal cell culture. Observed toxicity most likely was associated with heterodimerization but not homodimerization of ZFNs; cytotoxicity was greatly reduced when ZFN were provided at lower concentrations. Future studies evaluating efficiency of Ctsd knockout, off-target effects on molecular level and long-term outcomes in vivo can be performed.…

Condición: New. Print on Demand pp. 20.

Condición: New. Print on Demand pp. 24.

Condición: New. Print on Demand pp. 20.

Condición: New. Print on Demand pp. 24.

Condición: New. PRINT ON DEMAND pp. 20.

Condición: New. PRINT ON DEMAND pp. 24.

Taschenbuch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Internship Report from the year 2012 in the subject Biology - Neurobiology, grade: 1,3, University of Göttingen (Max Plank Institute for Experimental Medicine / Department of Neurogenetics), language: English, abstract: Tetraspanin2 (Tspan2) is a member of the tetraspan/transmembrane4 superfamily restricted to the nervous system. The abundance of Tspan2 is low in physiological condition but increases greatly in myelin of PLP-deficient mice which may indicate its' compensatory functions. Our experiments show that Tspan2 has no effect on delay of myelination at P14; young and old mice lacking Tspan2 has the same g-ratio as wild type. However, the quantity of unhealthy axons and axonal swellings are higher in aged animals lacking both PLP and Tspan2 than in single PLP knockout. Furthermore,the absence of PLP causes the decrease in the number of axons and rise in axon diameter; lack of Tspan2 alone leads to decrease in quantity of 0.4-0.7 µm diameter axons in 40 weeks old mice. These findings point to a possible auxiliary role of Tspan2 in support of axonal transport and long-term axon preservation in PLP null condition. 20 pp. Englisch. …

Taschenbuch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Project Report from the year 2013 in the subject Biology - Neurobiology, grade: 1.0, University of Göttingen, language: English, abstract: Genetic engineering is known as a powerful technique for basic research and clinical applications. Recent progress in development of zinc-finger nucleases (ZFNs), which combine the DNA cleavage ability of Fok1 restriction enzyme with highly specific recognition properties of zinc-finger motifs, allows to improve efficiency and to broaden the field of use of genome editing. Here, we demonstrate our initial results in generating novel tools for Cathepsin D gene knockout in neurons based on ZFNs technology and mediated by adeno-associated virus (AAV) vectors. Pairs of AAV-ZFNs were produced and demonstrated the robust expression of nucleases in neuronal cell culture. Observed toxicity most likely was associated with heterodimerization but not homodimerization of ZFNs; cytotoxicity was greatly reduced when ZFN were provided at lower concentrations. Future studies evaluating efficiency of Ctsd knockout, off-target effects on molecular level and long-term outcomes in vivo can be performed. 24 pp. Englisch.…

Condición: New. Print on Demand pp. 24 424:B&W 5.83 x 8.27 in or 210 x 148 mm (A5) Perfect Bound on Creme w/Matte Lam.

Condición: New. Print on Demand pp. 24.

Condición: New. PRINT ON DEMAND pp. 24.

Taschenbuch. Condición: Neu. Tetraspanin2 is a candidate for compensation of PLP functions | Maryna Psol | Taschenbuch | 20 S. | Englisch | 2014 | GRIN Verlag | EAN 9783656513834 | Verantwortliche Person für die EU: GRIN Publishing GmbH, Waltherstr. 23, 80337 München, info[at]grin[dot]com | Anbieter: preigu Print on Demand.…

Taschenbuch. Condición: Neu. Genetic knockout of Cathepsin D using zinc-finger nucleases delivered by AAV vectors | Maryna Psol | Taschenbuch | 24 S. | Englisch | 2013 | GRIN Verlag | EAN 9783656525035 | Verantwortliche Person für die EU: GRIN Publishing GmbH, Waltherstr. 23, 80337 München, info[at]grin[dot]com | Anbieter: preigu Print on Demand. …