Isbn: 9786208430412 - use of real-time rt-pcr: use of real-time rt-pcr for quantitation of fmdv rna in clinical samples

Taschenbuch. Condición: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Foot-and Mouth Disease virus (FMDV) is diagnosed by virus isolation test combined with antigen ELISA and recently one step RT-PCR. Use of fluorogenic RT-PCR is getting more and more importance for rapid detection of FMDV from clinical samples that cannot be processed by regular virus isolation tests and antigen ELISA. Quantitative real-time RT-PCR (qRT-PCR) combines Reverse Transcription with PCR amplification and detection of positive amplification using fluorescent probes like TaqMan ® probe. In the present study, a TaqMan® probe based qRT-PCR was standardized and used for detection of FMDV genome from clinical samples including nasal secretions, plasma and oesophago-pharyngeal fluids (probang samples). The initial standardization was made using different serial dilutions of a FMDV isolate OTNN 24/84 (106.8 TCID50) and 102 virus could be identified using the TaqMan® qRT-PCR. RNA standards were synthesized in vitro from a plasmid containing 110 base pair of 3D region of a FMDV type O isolate.…

USE OF REAL-TIME RT-PCR

N. Vijayalakshmi

Idioma: Inglés

Editorial: LAP LAMBERT Academic Publishing Mär 2025, 2025

6208430410 / 9786208430412

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Librería: buchversandmimpf2000, Emtmannsberg, BAYE, Alemaniabuchversandmimpf2000

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EUR 43,90

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Taschenbuch. Condición: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Foot-and Mouth Disease virus (FMDV) is diagnosed by virus isolation test combined with antigen ELISA and recently one step RT-PCR. Use of fluorogenic RT-PCR is getting more and more importance for rapid detection of FMDV from clinical samples that cannot be processed by regular virus isolation tests and antigen ELISA. Quantitative real-time RT-PCR (qRT-PCR) combines Reverse Transcription with PCR amplification and detection of positive amplification using fluorescent probes like TaqMan ® probe. In the present study, a TaqMan® probe based qRT-PCR was standardized and used for detection of FMDV genome from clinical samples including nasal secretions, plasma and oesophago-pharyngeal fluids (probang samples). The initial standardization was made using different serial dilutions of a FMDV isolate OTNN 24/84 (106.8 TCID50) and 102 virus could be identified using the TaqMan® qRT-PCR. RNA standards were synthesized in vitro from a plasmid containing 110 base pair of 3D region of a FMDV type O isolate.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 64 pp. Englisch.…

Isbn: 9786208430412 - use of real-time rt-pcr: use of real-time rt-pcr for quantitation of fmdv rna in clinical samples (11 resultados)

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Use of Real-Time Rt-PCR

USE OF REAL-TIME RT-PCR: USE OF REAL-TIME RT-PCR FOR QUANTITATION OF FMDV RNA IN CLINICAL SAMPLES

Use of Real-Time Rt-PCR

USE OF REAL-TIME RT-PCR: USE OF REAL-TIME RT-PCR FOR QUANTITATION OF FMDV RNA IN CLINICAL SAMPLES

Use of Real-Time Rt-PCR

USE OF REAL-TIME RT-PCR | USE OF REAL-TIME RT-PCR FOR QUANTITATION OF FMDV RNA IN CLINICAL SAMPLES

USE OF REAL-TIME RT-PCR

Use of Real-Time Rt-PCR

Use of Real-Time Rt-PCR

USE OF REAL-TIME RT-PCR : USE OF REAL-TIME RT-PCR FOR QUANTITATION OF FMDV RNA IN CLINICAL SAMPLES

USE OF REAL-TIME RT-PCR