9786207486908 - multiplex high-resolution melting analysis as a diagnostic tool: detection of carbapenems resistant genes in klebsiella pneumoniae isolated from iraqi patients de hamad, shahad tariq; ghaima, kais kassim (7 resultados)
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Taschenbuch. Condición: Neu. Multiplex High-Resolution Melting Analysis as a Diagnostic Tool | Detection of Carbapenems resistant genes in Klebsiella pneumoniae isolated from Iraqi patients | Shahad Tariq Hamad (u. a.) | Taschenbuch | Englisch | 2024 | LAP LAMBERT Academic Publishing | EAN 9786207486908 | Verantwortliche Person…für die EU: SIA OmniScriptum Publishing, Brivibas Gatve 197, 1039 RIGA, LETTLAND, customerservice[at]vdm-vsg[dot]de | Anbieter: preigu.
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Taschenbuch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 188 pp. Englisch.
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Librería: Biblios, frankfurt am main, HESSE, AlemaniaBiblios
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Taschenbuch. Condición: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This study was aimed to develop a multiplex RT-PCR with high resolution melting (HRM) analysis for the rapid and accurate detection of five carbapenemasehousekeeping gene 16S rRNA. Six pairs of primers and RT-PCR conditions were designe…d according to Beacon designer software. The identification of the genes was conducted by melting curves and temperatures with Multiplex RT-PCR using a dedicated HRM reagent. This is the first assay detection the main carbapenemase genes into a two-tube assay, enabling the simultaneous detection of 5 of the most important markers of resistance to 3rd generation cephalosporins and carbapenems.In the first panel tube, the reaction included bla-IMP, bla-VIM and bla-NDM. The results revealed that the melting temperature (TM) values were (81.1 oC, 84.5 oC and 86.1 oC), respectively, while the second panel tube contain the reaction of 16S rRNA,bla-OXA 48, and bla-KPC . The the melting temperature (TM) values were (84.5 oC ,81.7 oC and 89.35 oC), respectively at in one run with 2 hours. Result most prevalent genes were bla-IMP 38.75% , bla-OXA48 36.25%, and bla-VIM 36.25% , followed by bla-KPC 21.25% , and bla-NDM 13.75%.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 188 pp. Englisch.
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Librería: AHA-BUCH GmbH, Einbeck, AlemaniaAHA-BUCH GmbH
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Taschenbuch. Condición: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - This study was aimed to develop a multiplex RT-PCR with high resolution melting (HRM) analysis for the rapid and accurate detection of five carbapenemasehousekeeping gene 16S rRNA. Six pairs of primers and RT-PCR conditions were designed… according to Beacon designer software. The identification of the genes was conducted by melting curves and temperatures with Multiplex RT-PCR using a dedicated HRM reagent. This is the first assay detection the main carbapenemase genes into a two-tube assay, enabling the simultaneous detection of 5 of the most important markers of resistance to 3rd generation cephalosporins and carbapenems.In the first panel tube, the reaction included bla-IMP, bla-VIM and bla-NDM. The results revealed that the melting temperature (TM) values were (81.1 oC, 84.5 oC and 86.1 oC), respectively, while the second panel tube contain the reaction of 16S rRNA,bla-OXA 48, and bla-KPC . The the melting temperature (TM) values were (84.5 oC ,81.7 oC and 89.35 oC), respectively at in one run with 2 hours. Result most prevalent genes were bla-IMP 38.75% , bla-OXA48 36.25%, and bla-VIM 36.25% , followed by bla-KPC 21.25% , and bla-NDM 13.75%.
