9786200532169 - Isolation of Cry and Vip genes from native Bacillus thuringiensis: Cry1 type and Vip3 type genes from native Bacillus thuringiensis de Allam, Uday Sankar; Kaur, Sar... (6 resultados)

Paperback. Condición: Brand New. 128 pages. 8.66x5.91x0.29 inches. In Stock.

Taschenbuch. Condición: Neu. Isolation of Cry and Vip genes from native Bacillus thuringiensis | Cry1 type and Vip3 type genes from native Bacillus thuringiensis | Uday Sankar Allam (u. a.) | Taschenbuch | 128 S. | Englisch | 2020 | LAP LAMBERT Academic Publishing | EAN 9786200532169 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu.

Taschenbuch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Bacillus thuringiensis (Bt) is a Gram positive spore-forming soil bacterium that produces parasporal crystal proteins or d-endotoxin proteins (encoded by the cry genes) and cytolytic proteins (encoded by the cyt genes) during sporulation specifically toxic to many agriculturally important insect species. Another novel class of insecticidal proteins secreted during the vegetative phase includes vegetative insecticidal proteins (vip) and secreted insecticidal protein (sip). In the present study, presence of lepidopteran specific cry1, cry3 and vip3A type genes was investigated by polymerase chain reaction (PCR) using specially designed gene specific primers. The cry1 gene family was found to be present in ten out of twenty four native Bt isolates, while none of the isolates showed the presence of cry3 gene family and vip3 genes. A full-length 3.5 Kb cry1Ac type of gene was amplified from native Bt isolate SK-729 and sequenced. The sequence of the cloned gene was 99% in identical to that of the previously reported cry1Ac2 gene except four nucleotides and two amino acids variation at the protein level. The sequence was deposited in the NCBI with accession. No DQ023296. 128 pp. Englisch.

Condición: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Allam Uday SankarPresently, Dr Uday Sankar Allam working as an Assistant Professor in the Dept. of Biotechnology, VS University, Nellore since 2013.Bacillus thuringiensis (Bt) is a Gram positive spore-forming soil bacterium that .

Taschenbuch. Condición: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Bacillus thuringiensis (Bt) is a Gram positive spore-forming soil bacterium that produces parasporal crystal proteins or d-endotoxin proteins (encoded by the cry genes) and cytolytic proteins (encoded by the cyt genes) during sporulation specifically toxic to many agriculturally important insect species. Another novel class of insecticidal proteins secreted during the vegetative phase includes vegetative insecticidal proteins (vip) and secreted insecticidal protein (sip). In the present study, presence of lepidopteran specific cry1, cry3 and vip3A type genes was investigated by polymerase chain reaction (PCR) using specially designed gene specific primers. The cry1 gene family was found to be present in ten out of twenty four native Bt isolates, while none of the isolates showed the presence of cry3 gene family and vip3 genes. A full-length 3.5 Kb cry1Ac type of gene was amplified from native Bt isolate SK-729 and sequenced. The sequence of the cloned gene was 99% in identical to that of the previously reported cry1Ac2 gene except four nucleotides and two amino acids variation at the protein level. The sequence was deposited in the NCBI with accession. No DQ023296.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 128 pp. Englisch.

Taschenbuch. Condición: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Bacillus thuringiensis (Bt) is a Gram positive spore-forming soil bacterium that produces parasporal crystal proteins or d-endotoxin proteins (encoded by the cry genes) and cytolytic proteins (encoded by the cyt genes) during sporulation specifically toxic to many agriculturally important insect species. Another novel class of insecticidal proteins secreted during the vegetative phase includes vegetative insecticidal proteins (vip) and secreted insecticidal protein (sip). In the present study, presence of lepidopteran specific cry1, cry3 and vip3A type genes was investigated by polymerase chain reaction (PCR) using specially designed gene specific primers. The cry1 gene family was found to be present in ten out of twenty four native Bt isolates, while none of the isolates showed the presence of cry3 gene family and vip3 genes. A full-length 3.5 Kb cry1Ac type of gene was amplified from native Bt isolate SK-729 and sequenced. The sequence of the cloned gene was 99% in identical to that of the previously reported cry1Ac2 gene except four nucleotides and two amino acids variation at the protein level. The sequence was deposited in the NCBI with accession. No DQ023296.