springer us nov 2019 (41 resultados)

Buch. Condición: Neu. Neuware -In this concise yet comprehensive book, the author discusses the principles of mass, momentum, and energy transport, and derives balance equations for single-component fluids and multicomponent mixtures based on the direct application of natural laws and principles of thermodynamics.Transport equations over control volumes are formulated with reference to the Reynolds transport equation, thereby circumventing the need for ad-hoc balances for open systems that are best justified in hindsight.Notable features with regard to mass transport include the interpretation of diffusion in mixtures in terms of species parcel motion and separation, the introduction of Fick¿s and fractional diffusion laws with reference to random molecular excursions, a detailed account of species and mixture kinematics and dynamics, and the discussion of partial stresses, energies, and entropies of individual mixture components.Key features of this book include:¿ The governing equations are derived from first principles based on the application of natural laws and principles of thermodynamics¿ Balances over control volumes are derived from rigorous equations governing material parcel property evolution¿ Fick¿s law, a fractional diffusion law, and other diffusion laws are discussed with reference to random walks¿ A detailed account of species and mixture kinematics and dynamics is presented for binary and multicomponent solutions¿ A tabulated summary of transport equations is presented in differential and integral forms, and an overview of classical thermodynamics is given in an appendix for a self-contained discourseC. Pozrikidis has taught at the University of California and the University of Massachusetts. He is the author of several books on theoretical and computational topics in science and engineering, applied mathematics, scientific computing, and computer science.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 308 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This book offers a relatively self-contained presentation of the fundamental results in categorical data analysis, which plays a central role among the statistical techniques applied in the social, political and behavioral sciences, as well as in marketing and medical and biological research. The methods applied are mainly aimed at understanding the structure of associations among variables and the effects of other variables on these interactions. A great advantage of studying categorical data analysis is that many concepts in statistics become transparent when discussed in a categorical data context, and, in many places, the book takes this opportunity to comment on general principles and methods in statistics, addressing not only the ¿how¿ but also the ¿why.¿ Assuming minimal background in calculus, linear algebra, probability theory and statistics, the book is designed to be used in upper-undergraduate and graduate-level courses in the field and in more general statistical methodology courses, as well as a self-study resource for researchers and professionals. The book covers such key issues as: higher order interactions among categorical variables; the use of the delta-method to correctly determine asymptotic standard errors for complex quantities reported in surveys; the fundamentals of the main theories of causal analysis based on observational data; the usefulness of the odds ratio as a measure of association; and a detailed discussion of log-linear models, including graphical models. The book contains over 200 problems, many of which may also be used as starting points for undergraduate research projects. The material can be used by students toward a variety of goals, depending on the degree of theory or application desired.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 300 pp. Englisch.

Buch. Condición: Neu. Neuware -This volume looks at--and discusses the techniques of--a range of areas in biology in which macrophage migration inhibitory factor (MIF) is studied. Topics covered in this book include methods to quantify MIF in cell supernatants and human samples, using ELISA and western blot; detection and analysis of intracellular MIF using flow cytometry; genotyping of human MIF polymorphisms, detection of MIF expression in a marine invertebrate using rapid amplification of cDNA RACE; studying the MIF receptor CD74 in tissues using immunohistochemistry; and studying MIF in models of disease such as asthma, Plasmodium infection, and gout. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, Macrophage Migration Inhibitory Factor: Methods and Protocols is a valuable resource that will help researchers gain a new understanding of MIF biology in health and disease.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 280 pp. Englisch.

Buch. Condición: Neu. Neuware -This book introduces parabolic wave equations, their key methods of numerical solution, and applications in seismology and ocean acoustics. The parabolic equation method provides an appealing combination of accuracy and efficiency for many nonseparable wave propagation problems in geophysics. While the parabolic equation method was pioneered in the 1940s by Leontovich and Fock who applied it to radio wave propagation in the atmosphere, it thrived in the 1970s due to its usefulness in seismology and ocean acoustics.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 148 pp. Englisch.

Buch. Condición: Neu. Neuware -This volume provides comprehensive methods from expert scientists working in the Chimeric Antigen Receptor T Cell (CAR-T Cell) field. Chapters guide readers through the state-of-art of CAR-T cell technology, CAR design and vector production, CAR-T cell generation and manufacturing, CAR-T cell characterization, and quality control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Chimeric Antigen Receptor T Cells: Development and Production aims to be useful in the production of CAR-T cells, especially for therapeutic purposes.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 288 pp. Englisch.

Buch. Condición: Neu. Neuware -This book aims to provide an introduction to the broad and dynamic subject of discrete energy problems and point configurations. Written by leading authorities on the topic, this treatise is designed with the graduate student and further explorers in mind. The presentation includes a chapter of preliminaries and an extensive Appendix that augments a course in Real Analysis and makes the text self-contained. Along with numerous attractive full-color images, the exposition conveys the beauty of the subject and its connection to several branches of mathematics, computational methods, and physical/biological applications.This work is destined to be a valuable research resource for such topics as packing and covering problems, generalizations of the famous Thomson Problem, and classical potential theory in Rd. It features three chapters dealing with point distributions on the sphere, including an extensive treatment of Delsarte¿Yudin¿Levenshtein linearprogramming methods for lower bounding energy, a thorough treatment of Cohn¿Kumar universality, and a comparison of 'popular methods' for uniformly distributing points on the two-dimensional sphere. Some unique features of the work are its treatment of Gauss-type kernels for periodic energy problems, its asymptotic analysis of minimizing point configurations for non-integrable Riesz potentials (the so-called Poppy-seed bagel theorems), its applications to the generation of non-structured grids of prescribed densities, and its closing chapter on optimal discrete measures for Chebyshev (polarization) problems.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 684 pp. Englisch.

Buch. Condición: Neu. Neuware -This volume describes modern approaches for the detection and analysis of inositol phosphates from various biological, clinical, and environmental samples. Chapters focus on methods to study enzymes that modify inositol signals, chemical biology of inositol phosphates, and methods to synthesize and purify inositol phosphates and their analogs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Inositol Phosphates: Methods and Protocol aims to ensure successful results in the further study of this vital field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 196 pp. Englisch.

Buch. Condición: Neu. Neuware -This second edition volume expands on the previous edition with a look at the latest techniques used to study plant hormone jasmonate (JA). The chapters in this book and are organized into three parts: Parts One and Two discuss the role of JA in plant physiology and development, and in plant-biotic interactions. Part Three talks about methods used by researchers to study jasmonate metabolism and signaling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, Jasmonate in Plant Biology: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers who are interested in learning more about this developing field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 240 pp. Englisch.

Buch. Condición: Neu. Neuware -This volume provides a comprehensive compilation of techniques and protocols used in plant and food carotenoid research. Chapters guide readers through seven major areas on core enzyme activities, analysis of carotenoid profiles, new imaging tools, synthesis and degradation dynamics, biotechnology, nutrition, and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Plant and Food Carotenoids: Methods and Protocols aims to be helpful to researchers of other disciplines that are impacted by carotenoids, including photosynthesis, biotechnology, food science, and nutrition.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 440 pp. Englisch.

Buch. Condición: Neu. Neuware -This volume explores the latest techniques and improved methods used to study important neurobiological and associated neuropathological conditions. Chapters cover topics such as the application of semiconductor quantum dots as photostable fluorophores used for labeling and tracking; GCaMP6 transcranial imaging to monitor neural activity; the patch-clamping technique allowing simultaneous monitoring of membrane currents and optical signals; and quantifying behavioral dysfunction caused by traumatic brain injury (TBI). In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and practical, Basic Neurobiology Techniques is a valuable resource for novel and expert researchers interested in expanding their knowledge of this developing field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 364 pp. Englisch.

Buch. Condición: Neu. Neuware -This volume provides detailed protocols and methodologies required to perform bioluminescent imaging in multiple stages, enabling the reader to integrate this technology into their laboratory-based imaging experiments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols to ensure successful results in the further study of this vital field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 260 pp. Englisch.

Buch. Condición: Neu. Neuware -This book provides numerous methods for identification, validation, and functional characterization of chimeric RNAs, herein described as any transcript which contains the nucleotide sequence of two distinct parental genes. Beginning with identification and characterization through bioinformatics tools, the book continues with experimental validation using RT-PCR, hybridization methods for RNA, and the use of mass spectrometry data for fusion protein validation, as well as functional and mechanistic studies of chimeric RNAs and translational applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 276 pp. Englisch.

Buch. Condición: Neu. Neuware -This volume covers a diverse collection of protocols currently being used by the Toxoplasma research community, and also looks at innovative methods that are pushing the boundaries of possibilities. Chapters in this book discuss topics such as isolation and genotyping of Toxoplasma gondii strains; assessing rhoptry secretion in T. gondii; plate-based quantification of stimulated Toxoplasma egress; methods to study ocular toxoplasmosis; and metabolic analysis of Toxoplasma gondii tachyzoites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Toxoplasma gondii: Methods and Protocols is a valuable resource for students or groups starting off in the field, as well as laboratories interested in implementing the latest techniques described in the book.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 484 pp. Englisch.

Buch. Condición: Neu. Neuware -This volume details the current understanding of roles and regulation on histidine phosphorylation, describing methods for the characterization of protein phosphorylation on histidine. Chapters guide readers through in vitro systems, cell-based systems, comprehensive background review articles on histidine kinases and phosphatases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Histidine Phosphorylation: Methods and Protocols aims to ensure successful results in the further study of this rapidly growing field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 268 pp. Englisch.

Buch. Condición: Neu. Neuware -This volume explores detailed methods and experimental protocols evaluating the effect of a compound or a mixture of compounds on the action of enzymes that are significant targets in pharmaceuticals. Consisting of three sections, the book delves into recent biocomputing and bioinformatics protocols, state-of-the art modern biophysical, electrophoretic, and chromatographic methods and high-throughput screening approaches, as well as detailed protocols and examples of the inhibition analysis and evaluation of selected enzymes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 304 pp. Englisch.

Buch. Condición: Neu. Neuware -Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. This volume provides a concise set of protocols for assessing basic neutrophil functions and investigating specialized areas in neutrophil biology. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. In the third edition of Neutrophils: Methods and Protocols, chapters retained from previous editions have been have been updated and include many new approaches. In addition, the Third Edition contains a number of new chapters that were not included in the first or second editions. Part I contains overviews of neutrophil biology, function, and disorders. Part II describes commonly used methods to isolate neutrophils from humans and other animal species. Part III details methods for investigating chemotaxis, transmigration, phagocytosis, and bactericidal activity. Part IV includes protocols that measure neutrophil apoptosis, calcium signal transduction, degranulation and detection of cytoplasmic granules, gene expression, transcription factors, and apoptosis. Part V provides multiple assays for measuring production of intracellular and/or extracellular reactive oxygen species, and a method that details use of the cell-free NADPH oxidase assay, an iconic assay for studies of the neutrophil NADPH oxidase. Part VI provides chapters that describe how to analyze formation and function of neutrophil extracellular traps. Neutrophil Methods and Protocols: Third Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.Features¿ Provides neutrophil protocols that are broadly applicable to leukocyte biology¿ Includes protocols for isolation of neutrophils from humans and animal species¿ Presents an interesting and informative overview of the field of neutrophil biology¿ Facilitates a better understanding of the role of neutrophils in host defenseReadershipMolecular and Cell Biologists, Immunologists, Infectious Disease Specialists, MicrobiologistsSpringer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 488 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This volume discusses certain epigenetic changes recognized in early carcinogenic lesions and different tumors, as well as factors that alter the epigenome and epigenetic profile such as diet, alcohol, immunity, circadian rhythm, and more. The chapters in this book further delve into this field and cover topics such as epigenome-based precision medicine in lung cancer; interplay between genetic and epigenetic changes in breast cancer subtypes; genetic regulation of PDCD1 gene in cancer immunology; and pyrosequencing methylation analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Cancer Epigenetics for Precision Medicine: Methods and Protocols is a valuable resource to help researchers and scientists identify these specific biomarkers and work towards the prevention, diagnosis, and prognosis of different cancers in the future.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 312 pp. Englisch.

Buch. Condición: Neu. Neuware -This book provides a toolkit of novel research approaches for investigators to study diabetic nephropathy, including critical experimental models from the fly to the fish, cells in culture, and in vivo mammalian approaches. The collection also explores powerful techniques to image the kidney, such as traditional histological techniques as well as electron, confocal, and two-photon microscopy, pathophysiology of the diabetic kidney, and gene editing and regenerative medicine. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 356 pp. Englisch.

Buch. Condición: Neu. Neuware -This book focuses on ion mobility-mass spectrometry (IM-MS) and informatics approaches to improve traditional analysis of molecules by providing fundamentals and protocols for exploiting the potential of state-of-the-art IM-MS technology for the most common analytical applications. The chapters have been organized into four parts, each dealing with a particular set of IM-MS applications: 1) metabolomics and lipidomics; 2) proteomics and glycomics; 3) imaging and ambient ionization IM-MS; and 4) bioinformatic solutions for analyzing IM-MS data and deriving CCS values. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 324 pp. Englisch.

Buch. Condición: Neu. Neuware -Modeling Uncertainty: An Examination of Stochastic Theory, Methods, and Applications , is a volume undertaken by the friends and colleagues of Sid Yakowitz in his honor. Fifty internationally known scholars have collectively contributed 30 papers on modeling uncertainty to this volume. Each of these papers was carefully reviewed and in the majority of cases the original submission was revised before being accepted for publication in the book. The papers cover a great variety of topics in probability, statistics, economics, stochastic optimization, control theory, regression analysis, simulation, stochastic programming, Markov decision process, application in the HIV context, and others. There are papers with a theoretical emphasis and others that focus on applications. A number of papers survey the work in a particular area and in a few papers the authors present their personal view of a topic. It is a book with a considerable number of expository articles, which are accessible to a nonexpert - a graduate student in mathematics, statistics, engineering, and economics departments, or just anyone with some mathematical background who is interested in a preliminary exposition of a particular topic. Many of the papers present the state of the art of a specific area or represent original contributions which advance the present state of knowledge. In sum, it is a book of considerable interest to a broad range of academic researchers and students of stochastic systems. 804 pp. Englisch.

Buch. Condición: Neu. Neuware -This volume details key protocols for developing strategies in immunotherapy. Chapters guide the readers through protocols related to various DNA, RNA and protein methods to reprogram the immune system, immune cells, analyzing the effect of the reprogrammed cells, and key methods to consider and analyze patients enrolled in clinical trials with novel immunotherapy regimens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Cell Reprogramming for Immunotherapy: Methods and Protocols aims to ensure successful results in the further study of this vital field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 356 pp. Englisch.