springer us jul 2020 (25 resultados)

Taschenbuch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -We all know what randomness is. Or do we Randomness turns out to be one of those concepts that works just fine on an everyday level, but becomes muddled upon close inspection. People familiar with quantum indeterminacy tell us that order is an illusion and that the world is fundamentally random. Yet these same people also say that randomness is an illusion: The appearance of randomness is only a sign of our ignorance and inability to detect the pattern.By applying mathematical thinking, mathematician Edward Beltrami removes much of the vagueness that encumbers the concept of randomness. You will discover how to quantify what would otherwise remain elusive. As the book progresses, you will see how mathematics provides a framework for unifying how chance is interpreted across diverse disciplines. Communication engineering, computer science, philosophy, physics, and psychology join mathematics in the discourse to illuminate different facets of the same idea.Thisbook will provoke, entertain, and inform by challenging your ideas about randomness, providing different interpretations of what this concept means, and showing how order and randomness are really two sides of the same mysterious coin.This second edition brings the question of randomness into the twenty-first century, adding compelling new topics such as quantum uncertainty, cognitive illusions caused by chance, Poisson processes, and Bayesian probability. An expanded technical notes section offers deeper explorations of a variety of mathematical concepts.On the first edition:I strongly recommend [What is Random ] to all who are interested in science and would like to see how the ideas of both theoretical mathematics and statistics have been observed and used in real life throughout history. The American Statistician 212 pp. Englisch.

Taschenbuch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume discusses different approaches to workflows for large volume electron microscopy - from preparation of samples to their imaging in a variety of microscopes - in some cases also applying correlative techniques. The chapters in this book cover topics such as correlative super resolution and electron microscopy to detect molecules in their native cellular context; low-threshold access to serial section arrays; improving serial blockface SEM by focal charge compensation; FIBSEM analysis of interfaces between hard technical devices and soft neuronal tissue; and image processing for volume electron microscopy. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and authoritative, Volume Microscopy: Multiscale Imaging with Photons, Electrons, and Ions is a valuable resource for novice and expert scientists interested in learning more about this evolving field. 324 pp. Englisch.

Taschenbuch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This fully updated book brings together protocols to arm stem cell biologists with tools and approaches to continue uncovering the intricacies and regulatory mechanisms underlying stem cell biology. Through various models and organ systems, the volume reflects the numerous recent advances in cell lineage and lineage tracking. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Imaging and Tracking Stem Cells: Methods and Protocols, Second Edition is an ideal guide for novices and experts alike who are working to expand our knowledge in the field of stem cells. 268 pp. Englisch.

Taschenbuch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template.Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism. 296 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book encapsulates the most up-to-date methods of the intestinal stem cell field and provides guidance on a variety of techniques for studying intestinal stem cells properties. Beginning with a section on in vitro techniques to study different aspects of the intestinal stem cellfunctions by innovative imaging and functional assays, the volume continues with chapters detailing the single-cell transcriptional profiling method, the isolation of intestinal crypts to generate and establish 3D organoids, as well as different animal models of gastrointestinal cancer and examples of the use of in vivo methods for studying intestinal tumor-initiating cells or cancer stem cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and state-of-the-art, Intestinal Stem Cells: Methods and Protocols aims to provide comprehensive and easy to follow protocols designed to be helpful to both seasoned researchers and newcomers to this dynamic field. 368 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book serves to introduce the technologies and applications of precision medicine. Beginning with a section on emerging experimental and bioinformatics technologies, the collection continues with chapters covering cancer studies, cardiovascular diseases, COPD and systemic lupus erythematosus, as well as the latest engineering and surgical developments in precision medicine. As a volume in the highly successful Methods in Molecular Biology series, this book presents key information from experts in the field.Authoritative and cutting-edge, Precision Medicine covers perspectives of precision medicine from basic research to clinical surgeries, from cancer to cardiovascular disease, from sequencing technology to big data analysis in the hope of broadening the horizons for researchers, engineers, and clinicians and accelerating the interdisciplinary precision medicine research and applications. 240 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This comprehensive laboratory manual describes the various protocols involved in Actinobacterial research. The content is divided into fifteen major sections, each of which is further divided into sub-sections describing the respective aim, principles, materials & methods, protocol, expected results and diagrams. Readers will find essential protocols for e.g. sample collection, isolation, characterization, analysis, profiling and evaluation of Actinobacteria for various applications. Gathering all relevant protocols concerning Actinobacteria, and written by a team of experienced Actinobacterial researchers, it is the first book of its kind. 260 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This thorough book aims to present the methods that have enabled the success of peptides and proteins in a wide variety of applications. It opens with a section on chemical tools applied to the production or engineering of peptides and proteins, and concludes with a collection of chapters on biological approaches used to engineer structure and function in peptides and proteins. As a book in the Springer Protocols Handbooks series, chapters include the kind of detailed descriptions and tips necessary for successful results in practice.Authoritative and practical, Peptide and Protein Engineering: From Concepts to Biotechnological Applications will be of great use to scientists in academia and industry seeking a better understanding of the emerging principles and methodologies in peptide and protein engineering. 304 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, and disease. Beginning with a section on in situ detection of RNA molecules using FISH techniques, the volume continues with parts exploring in vivo imaging of RNA transport and localization, imaging and analysis of RNA uptake and transport between cells, identification and analysis of RNA-binding proteins, guide RNAs in genome editing, as well as other specific analytical techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, RNA Tagging: Methods and Protocols serves as a vital reference for researchers looking to further the increasingly important research in RNA biology. 504 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume focuses on RNAs interacting with chromatin and their function. Chapters guide readers through transcription, splicing, non-coding RNA function and manipulation of gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, RNA-Chromatin Interactions: Methods and Protocols aims to be a starting-point to expand researchers experimental approaches towards the numerous outstanding questions in this new and expanding field. 276 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume looks at all aspects of manipulation of Leptospira spp. from strain isolation to the latest techniques used to study the pathogenesis of leptospirosis. The chapters in this book cover topics such as the procedure to cultivate and isolate leptospires from both clinical and environmental samples; using methods like whole genome sequencing and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry to identify bacterial species; tools for gene inactivation and in vitro and in vivo assays to study the pathogenesis of leptospirosis; and the use of hamsters to evaluate leptospiral virulence and vaccine candidates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Leptospira spp.: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field and these fascinating organisms. 304 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides cutting edge protocols for using optogenetic and opto-chemical techniques across a wide spectrum of applications. Chapters guide readers through methods in neuro-optogenetics, including methods to dissect local and long-range connectivity ex vivo, investigate G-protein coupled receptors, study astrocyte-neuron networks in vitro and in vivo, employ upconversion nanoparticles for transcranial optogenetic stimulation, and use Adeno-associated virus (AAV) for delivery of optogenetic constructs. At the same time, this book covers a range of methods for non-neuronal optogenetics, including various applications of light-oxygen-voltage domains or cryptochromes for control of nucleocytoplasmic transport, transgene expression and chromatin recruitment. The book further presents protocols for traceless light-control of gene expression with melanopsin, light-inducible cAMP production, use of light-regulated receptor tyrosine kinases (Opto-RTKs), synthesis of light-dependent biomaterials, and highlights emerging tools at the intersection of optogenetics and CRISPR. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Photoswitching Proteins: Methods and Protocols aims to provide a comprehensive list of methods and tools to investigate highly dynamic pathways and behaviors in cells and animals using optogenetics. 300 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides protocols designed to study the function and the structure of diverse ribozymes. Chapters guide readers through different techniques to identify and characterize new ribozymes and methods to use ribozymes to alter the function of CRISPR-based guide RNAs, AgoshRNAs and aptamers or to study RNA capping and long non-coding RNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Ribozymes: Methods and Protocols aims to help in accelerating ribozyme research and inspiring others to develop new methods to study ribozyme structure and function. 320 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book illustrates experimental and computational methodologies used to achieve cost effective biological processes for the production of fuels and biochemicals through multiple approaches to increasing yield, titers, and productivity in a robust host. The volume includes the most recent and cutting-edge aspects of pathway engineering, flux analysis, and metabolic enzyme engineering. Each chapter highlights the complexity and challenges of the problem as well as the methods used to solve this problem or changes needed in current methods. As a part of the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that gives researchers a much needed boost.Authoritative and practical, Metabolic Pathway Engineering benefits not only scientists working on more fundamental aspects of this endeavor but also those in the biochemical industry working on strain engineering for robust industrial processes. 248 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition details techniques for the study of cargo trafficking throughendosomes. New and updated chapters guide readers through methods and protocols on the structural aspects of plantendosomes, combined biochemical, omics, imaging approaches to study the dynamics and contents of endosomal compartments. Additional chapters are dedicated to the analysis of lipids onendosomes and the identification and analysis of lipid binding proteins and lipid-binding domains relevant for the study of plantendosomes.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,PlantEndosomes: Methods and Protocols, Second Editionaims to ensure successful results in the further study of this vital field. 212 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed new edition combines a wide selection of experimental methods for plant cold acclimation, deacclimation, and cold memory, ranging from the whole plant level of ecology and breeding to molecular profiling and the detailed analysis of specific proteins, with many levels of investigated complexity in between. The collection updates methodology from the first edition while adding chapters on numerous key advances in the field. Written for the highly successful Method in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Plant Cold Acclimation: Methods and Protocols, Second Edition seeks to aid researchers, both new and experienced, in entering this exciting field of research or broadening the scope of their investigations. 348 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides a complete overview ofkupffercell biology, from isolation to phenotyping. Chapters guide readers through methods and protocolson phenotyping,includingRNA sequencing, Masscytometry and3Dmicroscopy,as well asgenemanipulationin vivo, and disease models from fatty liver disease to cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, KupfferCells: Methods and Protocols aimstoensuresuccessfulinvestigationofthisgrowingfield. 172 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume looks at all aspects of manipulation of Leptospira spp. from strain isolation to the latest techniques used to study the pathogenesis of leptospirosis. The chapters in this book cover topics such as the procedure to cultivate and isolate leptospires from both clinical and environmental samples; using methods like whole genome sequencing and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry to identify bacterial species; tools for gene inactivation and in vitro and in vivo assays to study the pathogenesis of leptospirosis; and the use of hamsters to evaluate leptospiral virulence and vaccine candidates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Leptospira spp.: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field and these fascinating organisms.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 304 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume provides cutting edge protocols for using optogenetic and opto-chemical techniques across a wide spectrum of applications. Chapters guide readers through methods in neuro-optogenetics, including methods to dissect local and long-range connectivity ex vivo, investigate G-protein coupled receptors, study astrocyte-neuron networks in vitro and in vivo, employ upconversion nanoparticles for transcranial optogenetic stimulation, and use Adeno-associated virus (AAV) for delivery of optogenetic constructs. At the same time, this book covers a range of methods for non-neuronal optogenetics, including various applications of light-oxygen-voltage domains or cryptochromes for control of nucleocytoplasmic transport, transgene expression and chromatin recruitment. The book further presents protocols for traceless light-control of gene expression with melanopsin, light-inducible cAMP production, use of light-regulated receptor tyrosine kinases (Opto-RTKs), synthesis of light-dependent biomaterials, and highlights emerging tools at the intersection of optogenetics and CRISPR. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Photoswitching Proteins: Methods and Protocols aims to provide a comprehensive list of methods and tools to investigate highly dynamic pathways and behaviors in cells and animals using optogenetics.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 300 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed new edition delves into recently developed protocols for those working on cell nucleus research, including some of the less well-explored areas of study. Like the previous editions, this book features contributions from top experts in their respective fields. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, The Nucleus, Third Edition will help students and postdocs become aware of vital available methodologies when planning their new projects. 232 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides an overview about main RNA editing mechanisms, focusing on their functions in physiological as well as pathological conditions. Chapters guide readers through state- of-the art methodologies to investigate RNA editing through wet and dry approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,RNA Editing: Methods and Protocols aims to ensure successful results in the further study of this vital field. 368 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This thorough new edition explores web servers and software for protein structure prediction and modeling that are freely available to the academic community. Taking into account the numerous advances in the computational protein structure prediction/modeling field, the book includes residue-contact prediction via deep learning, a wide variety of protein docking models, as well as cryo-electron microscopy (cryo-EM) techniques. Written by renowned experts in the field and for the highly successful Methods in Molecular Biology series, chapters include the kind of key detail and implementation advice necessary for researchers to achieve optimal results in their own work.Authoritative and fully updated, Protein Structure Prediction, Fourth Edition is a practical and immediately useful guide for biology researchers working toward modeling protein structures. 372 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 972 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume of the 'Encyclopedia of Complexity and Systems Science, Second Edition' (ECSS), introduces the fundamental physical and mathematical concepts underlying the theory of complex physical, chemical, and biological systems. Numerous applications illustrate how these concepts explain observed phenomena in our daily lives, which range from spatio-temporal patterns in fluids from atmospheric turbulence in hurricanes and tornadoes to feedback dynamics of laser intensity to structures in cities and rhythms in the brain. The spontaneous formation of well-organized structures out of microscopic system components and their interactions is one of the most fascinating and challenging phenomena for scientists to understand. Biological systems may also exhibit organized structures emanating from interactions of cells and their networks. For instance, underlying structures in the brain emerge as certain mental states, the ability to coordinate movement, or pathologies such as tremor or epileptic seizures. When we try to explain or understand these extremely complex biological phenomena, it is natural to ask whether analogous processes of self-organization may be found in much simpler systems of the inanimate world. In recent decades, it has become increasingly evident that there exist numerous examples in physical and chemical systems in which well-organized spatio-temporal structures arise out of disordered states. As in living organisms, the functioning of these systems can be maintained only by a flux of energy (and matter) through them. Synergetics combines elements from physics and mathematics to explain how a diversity of systems obey the same basic principles. All chapters in this volume have been thoroughly revised and updated from the first edition of ECSS. The second edition also includes new or expanded coverage of such topics as chaotic dynamics in laser systems and neurons, novel insights into the relation of classical chaos and quantum dynamics, and how noise in the brain tunes observed neural activity and controls animal and human behavior. 472 pp. Englisch.

Buch. Condición: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume of the ¿Encyclopedia of Complexity and Systems Science, Second Edition¿ (ECSS), introduces the fundamental physical and mathematical concepts underlying the theory of complex physical, chemical, and biological systems. Numerous applications illustrate how these concepts explain observed phenomena in our daily lives, which range from spatio-temporal patterns in fluids from atmospheric turbulence in hurricanes and tornadoes to feedback dynamics of laser intensity to structures in cities and rhythms in the brain. The spontaneous formation of well-organized structures out of microscopic system components and their interactions is one of the most fascinating and challenging phenomena for scientists to understand. Biological systems may also exhibit organized structures emanating from interactions of cells and their networks. For instance, underlying structures in the brain emerge as certain mental states, the ability to coordinate movement, or pathologies such as tremor or epileptic seizures. When we try to explain or understand these extremely complex biological phenomena, it is natural to ask whether analogous processes of self-organization may be found in much simpler systems of the inanimate world. In recent decades, it has become increasingly evident that there exist numerous examples in physical and chemical systems in which well-organized spatio-temporal structures arise out of disordered states. As in living organisms, the functioning of these systems can be maintained only by a flux of energy (and matter) through them. Synergetics combines elements from physics and mathematics to explain how a diversity of systems obey the same basic principles. All chapters in this volume have been thoroughly revised and updated from the first edition of ECSS. The second edition also includes new or expanded coverage of such topics as chaotic dynamics in laser systems and neurons, novel insights into the relation of classical chaos and quantum dynamics, and how noise in the brain tunes observed neural activity and controls animal and human behavior.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 472 pp. Englisch.