Sensor Technology for Water Quality Monitoring : Fiber Optic Sensor

Idioma: inglés

Editorial: IWA Publishing Sep 2004, 2004

1843396777 / 9781843396772

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Librería: AHA-BUCH GmbH, Einbeck, AlemaniaAHA-BUCH GmbH

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Descripción del artículo del vendedor

Neuware - Two methods for the detection of important human pathogens, Cryptosporidium parvum and Helicobacter pylori, were investigated: a fiber optic biosensor, and real time PCR. The mechanism for specific detection in both methods is recognition of specific DNA sequences in the target organisms. The biosensor that was used, the Analyte 2000, was originally developed for the detection of chemicals. It utilizes a fiber optic wave guide that propagates an evanescent light wave of very specific wavelength. The light excites fluorescent molecules bound to the waveguide, but not in the bulk solution, which theoretically enhances signal while reducing background interference. Attempts to develop this system for the detection of DNA were not successful due to poor detection of the target molecules. An assay analogous to a sandwich immunoassay was designed for use on the Analyte 2000. Specific oligonucleotide probes were designed to bind to the waveguides via biotin-streptavidin interaction, and were used to capture the target DNA. Pure target DNA representing unique genes in the organisms were synthesized by PCR. Detection of captured DNA was then attempted using an oligonucleotide detection probe designed to bind to the target. Two detection systems were employed: an indirect signal amplification system based on biotin-tyramide deposition, or direct detection of fluorescent signal from Cy-5 molecules. In all experiments performed there was very little difference between the signal generated with or without the target molecules. Many experiments were conducted to attempt to identify reasons for the poor signal. Signal was only of any significance when target amplicons were internally labeled with Cy-5 by PCR. Real time PCR as a method to detect the pathogens was also investigated. Though the PCR technique itself is very rapid, DNA extraction and purification requires preparation time. Filtration of up to one liter of well water, followed by concentration and 'cleaning' Helicobacter pylori cells by immunomagnetic separation, was used to detect H. pylori seeded in a water source. Following cell lysis, the extracted DNA could be used directly in conventional PCR targeting the 16S rRNA gene to detect less than 265 cells per liter of water. DNA purification was not required for this level of detection. Initial studies to amplify lysed cells by real time PCR indicated that an incorrect product was made. When purified DNA was used for real time PCR, the correct product was produced from DNA representing as few as 100 cells. …

N° de ref. del artículo 9781843396772

Título
Sensor Technology for Water Quality Monitoring : Fiber Optic Sensor
Autor
A. C. Cannons
Editorial
IWA Publishing Sep 2004
Año de publicación
2004
Estado
Neu
Encuadernación
Taschenbuch
Idioma
inglés
ISBN 10
1843396777
ISBN 13
9781843396772
Peso del artículo
212 gramos
Dimensiones
279x211x8 mm

AHA-BUCH GmbH

Einbeck, Alemania

Vendedor de 5 estrellas

Vendedor de IberLibro desde 14 de agosto de 2006

Tarifas de envío de Alemania a Estados Unidos de America

ArtículoDe 7 a 10 días hábilesDe 5 a 7 días hábiles
Primer artículoEUR 35,00EUR 45,00
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AHA-BUCH GmbH

Garlebsen 48
Einbeck, Alemania 37574

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AHA-BUCH GmbH
representado por el director gerente Christel Glass
Garlebsen 48
37574 Einbeck
Alemania
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E-Mail: abebooks@aha-buch.de
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