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Mapping of RAPD primers on chromosome 2A of common wheat: Mapping of chromosome 2A of common wheat triticum aestivum - Tapa blanda

Parveen, Khamsa; Ahmad, Israr

 
9783846550199: Mapping of RAPD primers on chromosome 2A of common wheat: Mapping of chromosome 2A of common wheat triticum aestivum
Tapa blanda
ISBN 10: 3846550191 ISBN 13: 9783846550199
Editorial: LAP LAMBERT Academic Publishing, 2012

Sinopsis

Common (bread) wheat is an allohexaploid species (Triticum aestivum L.) with 3 compensating genomes AABBDD (2n=6x=42 chromosomes). Traditionally genetic and physical mapping in this most important cereal crop has been based on molecular markers (RFLPs, PCR, Insitu hubridisation etc). The recent development in producing deletion lines of common wheat (using Aegilops cylindricum genome) has revolutionized the physical mapping of wheat chromosomes. During present study, deletion lines of chromosomes 2A of common wheat were used to identify RAPD (Randomly Amplified Polymorphic DNA) based molecular markers specific for short and/or long arm of chromosome 2A. Out of the 7 RAPD primers used, OPA-07 showed useful polymorphism. By comparing the C-banding karyotype of the deletion lines, it is inferred that the primer (OPA-07) anneals to the distal half of the long arm of chromosome 2A.

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Reseña del editor

Common (bread) wheat is an allohexaploid species (Triticum aestivum L.) with 3 compensating genomes AABBDD (2n=6x=42 chromosomes). Traditionally genetic and physical mapping in this most important cereal crop has been based on molecular markers (RFLPs, PCR, Insitu hubridisation etc). The recent development in producing deletion lines of common wheat (using Aegilops cylindricum genome) has revolutionized the physical mapping of wheat chromosomes. During present study, deletion lines of chromosomes 2A of common wheat were used to identify RAPD (Randomly Amplified Polymorphic DNA) based molecular markers specific for short and/or long arm of chromosome 2A. Out of the 7 RAPD primers used, OPA-07 showed useful polymorphism. By comparing the C-banding karyotype of the deletion lines, it is inferred that the primer (OPA-07) anneals to the distal half of the long arm of chromosome 2A.

Biografía del autor

I Miss Khamsa Parveen did my Master in Genetics from Hazara University Mansehra Pakistan. My research topic was “MAPPING OF RANDOMLY AMPLIFIED POLYMORPHIC DNA PRIMERS (RAPD) ON CHROMOSOME 2A OF COMMON WHEAT TRITICUM AESTIVUM (L). I did my research work in Functional Genomic Lab (FGL)of the said department.

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Editorial
LAP LAMBERT Academic Publishing
Año de publicación
2012
Idioma
Inglés
ISBN 10 
3846550191
ISBN 13 
9783846550199
Encuadernación
Tapa blanda
Número de páginas
68
Contacto del fabricante
no disponible
Persona responsable
no disponible