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9783642629785: In Living Color: Protocols in Flow Cytometry and Cell Sorting (Springer Lab Manuals)

Sinopsis

Advances in the field of cell biology have always been closely related to the development of quantitative analytical methods that can be applied to individual cells or cell organelles. Almost from the early stages following the invention of the microscope, the investigator has been keenly interested in obtaining informa­ tion on the functionality of single cells and how cells perform under different sets of experimental conditions. Although cells could be viewed in the microscope for a few hundred years, only since the relatively recent application of autoradiography did we come to realize that, although cells may visually appear very much alike, they are quite different in their functional capacity. The quest to understand these differences in a cell population lead to a new series of techniques for labeling and quantitating DNA content and similar approaches have driven the develop­ ment of methods for analyzing various other cellular properties. The development of new analytical techniques follows the age old pattern of applying successes of the past with current inno­ vation, logic and new biological information. Results from auto­ radiography expanded the concept of the cell cycle from inter­ phase and mitosis to the more definitive GO/GI, Sand G2/M phases. This new knowledge lead to the development of techno­ logy to measure and analyze various parameters related to the cell cycle.

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Reseña del editor

A contribution towards making this increasingly valuable technology accessible to researchers, including the students, post-doctoral scholars, and technicians gathering the knowledge inherent in this integration between analysis and physical isolation/purification methodologies. A step-by-step approach to the methodology for measuring various attributes demonstrated in the particular cells of interest is provided, as is a myriad of resources to fuel the curiosity and answer questions of both new and adept users. This book stems from the editors'experiences managing flow cytometry/cell sorting core facilities for the emerging researchers, in particular in developmental, cellular, and molecular biology.

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9783540651499: In Living Color: Protocols in Flow Cytometry and Cell Sorting (Springer Lab Manuals)

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ISBN 10:  3540651497 ISBN 13:  9783540651499
Editorial: Springer-Verlag Berlin and Heide..., 2000
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ISBN 10: 3642629784 ISBN 13: 9783642629785
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Diamond, Rochelle A.|DeMaggio, Susan
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Condición: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Flow cytometry and cell sorting are becoming increasingly important in biological researchStep-by-step approach to the methodology for measuring various cellular attributesA contribution towards making this increasingly valuable technology accessibl. Nº de ref. del artículo: 5065322

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Taschenbuch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -A contribution towards making this increasingly valuable technology accessible to researchers, including the students, post-doctoral scholars, and technicians gathering the knowledge inherent in this integration between analysis and physical isolation/purification methodologies. A step-by-step approach to the methodology for measuring various attributes demonstrated in the particular cells of interest is provided, as is a myriad of resources to fuel the curiosity and answer questions of both new and adept users. This book stems from the editors'experiences managing flow cytometry/cell sorting core facilities for the emerging researchers, in particular in developmental, cellular, and molecular biology. 864 pp. Englisch. Nº de ref. del artículo: 9783642629785

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Taschenbuch. Condición: Neu. Druck auf Anfrage Neuware - Printed after ordering - Advances in the field of cell biology have always been closely related to the development of quantitative analytical methods that can be applied to individual cells or cell organelles. Almost from the early stages following the invention of the microscope, the investigator has been keenly interested in obtaining informa tion on the functionality of single cells and how cells perform under different sets of experimental conditions. Although cells could be viewed in the microscope for a few hundred years, only since the relatively recent application of autoradiography did we come to realize that, although cells may visually appear very much alike, they are quite different in their functional capacity. The quest to understand these differences in a cell population lead to a new series of techniques for labeling and quantitating DNA content and similar approaches have driven the develop ment of methods for analyzing various other cellular properties. The development of new analytical techniques follows the age old pattern of applying successes of the past with current inno vation, logic and new biological information. Results from auto radiography expanded the concept of the cell cycle from inter phase and mitosis to the more definitive GO/GI, Sand G2/M phases. This new knowledge lead to the development of techno logy to measure and analyze various parameters related to the cell cycle. Nº de ref. del artículo: 9783642629785

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Taschenbuch. Condición: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Advances in the field of cell biology have always been closely related to the development of quantitative analytical methods that can be applied to individual cells or cell organelles. Almost from the early stages following the invention of the microscope, the investigator has been keenly interested in obtaining informa tion on the functionality of single cells and how cells perform under different sets of experimental conditions. Although cells could be viewed in the microscope for a few hundred years, only since the relatively recent application of autoradiography did we come to realize that, although cells may visually appear very much alike, they are quite different in their functional capacity. The quest to understand these differences in a cell population lead to a new series of techniques for labeling and quantitating DNA content and similar approaches have driven the develop ment of methods for analyzing various other cellular properties. The development of new analytical techniques follows the age old pattern of applying successes of the past with current inno vation, logic and new biological information. Results from auto radiography expanded the concept of the cell cycle from inter phase and mitosis to the more definitive GO/GI, Sand G2/M phases. This new knowledge lead to the development of techno logy to measure and analyze various parameters related to the cell cycle.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 864 pp. Englisch. Nº de ref. del artículo: 9783642629785

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