Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice.
"Sinopsis" puede pertenecer a otra edición de este libro.
Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice.
"Sobre este título" puede pertenecer a otra edición de este libro.
Librería: Brook Bookstore On Demand, Napoli, NA, Italia
Condición: new. Questo è un articolo print on demand. Nº de ref. del artículo: NHL4X5NIMY
Cantidad disponible: Más de 20 disponibles
Librería: Ria Christie Collections, Uxbridge, Reino Unido
Condición: New. In. Nº de ref. del artículo: ria9783642477331_new
Cantidad disponible: Más de 20 disponibles
Librería: Chiron Media, Wallingford, Reino Unido
Paperback. Condición: New. Nº de ref. del artículo: 6666-IUK-9783642477331
Cantidad disponible: 10 disponibles
Librería: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Alemania
Taschenbuch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice. 256 pp. Englisch. Nº de ref. del artículo: 9783642477331
Cantidad disponible: 2 disponibles
Librería: Revaluation Books, Exeter, Reino Unido
Paperback. Condición: Brand New. 2002 edition. 256 pages. 9.21x6.14x0.54 inches. In Stock. Nº de ref. del artículo: x-364247733X
Cantidad disponible: 2 disponibles
Librería: moluna, Greven, Alemania
Condición: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. A comprehensive, up-to-date guide on all aspects of synthesis and screening of genetic librariesDetailed step-by-step protocols and extensive hands-on advice gleaned from the authors experiencePerfectly suited for experienced researchers and gradua. Nº de ref. del artículo: 5062205
Cantidad disponible: Más de 20 disponibles
Librería: buchversandmimpf2000, Emtmannsberg, BAYE, Alemania
Taschenbuch. Condición: Neu. This item is printed on demand - Print on Demand Titel. Neuware -PCR and cDNA Cloning Techniques.- 1 Strategies for cDNA Cling and Mapping RNA Transcripts.- 2 Cloning PCR Products.- 3 Site Directed Deletion, Insertion, and Substitution Using PCR.- 4 Long RT-PCR Cloning - Amplification of Full-Length Enterovirus Genomes.- 5 Construction of cDNA Libraries from Small Quantities of Total RNA Using Template Switching Catalyzed by M-MLV Reverse Transcriptase.- Subtractive and Differential Display Techniques.- 6 Subtractive Hybridization and cDNA Cloning.- 7 Differential Display to Identify Steroid-Induced Genes in Endocrine Cells.- Specialized Cloning and Library Screening Strategies.- 8 Two Hyrid cDNA Cloning.- 9 Yeast One and Two Hyrid cDNA Cloning.- 10 High-Throughput Library Screening by Fluorescent Hybridisations on Gridded Membranes.- 11 Identification of Cell Targeting Ligands Using Random Peptide-Presenting Phage Libraries.Springer-Verlag KG, Sachsenplatz 4-6, 1201 Wien 256 pp. Englisch. Nº de ref. del artículo: 9783642477331
Cantidad disponible: 1 disponibles
Librería: AHA-BUCH GmbH, Einbeck, Alemania
Taschenbuch. Condición: Neu. Druck auf Anfrage Neuware - Printed after ordering - Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice. Nº de ref. del artículo: 9783642477331
Cantidad disponible: 1 disponibles