Genetic Library Construction and Screening: Advanced Techniques and Applications (Springer Lab Manuals) - Tapa blanda

 
9783642477331: Genetic Library Construction and Screening: Advanced Techniques and Applications (Springer Lab Manuals)

Sinopsis

Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice.

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Reseña del editor

Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice.

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9783540672784: Genetic Library Construction and Screening: Advanced Techniques and Applications (Springer Lab Manuals)

Edición Destacada

ISBN 10:  3540672788 ISBN 13:  9783540672784
Editorial: Springer-Verlag Berlin and Heide..., 2002
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