PCR Topics: Usage of Polymerase Chain Reaction in Genetic and Infectious Diseases - Tapa blanda

Rolfs, Arndt

 
9783540529347: PCR Topics: Usage of Polymerase Chain Reaction in Genetic and Infectious Diseases
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ISBN 10: 3540529349 ISBN 13: 9783540529347
Editorial: Springer, 1991

Sinopsis

The Polymerase chain reaction (PCR) has revolutionized the technical development of molecular biology and immunology. It is a very simple method for amplifying nucleic acids in vitro. During the last two years PCR has mostly been used on investigations of oncogenes, tumor associated translocations, retroviral structures, HLA polymorphism and lymphokines. Because this method will make it possible to identify previously unknown structures it is particulary suitable for diagnosis in the broad field of degenerative and inflammatory diseases of the nervous system. Here we have two problems: a small amount of material and the necessity of rapid diagnostic procedures in inflammatory infections. PCR seems to be the technique which could overcome these difficulties.

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Reseña del editor

PCR, developed at Cetus Corporation/USA by Henry A. Erlich, Kary Mullis and Randall K. Saiki, is a very simple method for amplifying nucleic acids in vitro. The realization of this idea bases on the repetition of a set of three different temperatures and yields an increase of the target structure up to a factor of 106 to 1012. Therefore, this technique is predisposed for safe analysis and characterization of DNA and RNA sequences of interest, even where the starting amount of material is enormously small. Because of its sensitivity, speed and versatility this method is particularly suitable for investigations of oncogenes, tumor associated translocations, retroviral sequences, lymphokines and mainly the broad field of degenerative and inflammatory diseases of nervous system. PCR seems to be the technique which could overcome the two most important problems in that field: very small amount of material combined with the necessity of rapid diagnostic procedures in inflammatory infections. "PCR topics" will give an actual overview of basic and applied research fields on usage of polymerase chain reaction. All contributions to this book have been presented at an international congress on "Usage of Polymerase chain reaction in genetic and infectious diseases" which took place in june 1990 in Berlin. The editors wish to thank all participants for their contributions. We offer our thanks and gratitude to our coworkers and especially to our technical assistents Barbara Trampenau, Mirjana Wiirdemann and Hannelore Leonhard.

Reseña del editor

The Polymerase chain reaction (PCR) has revolutionized the technical development of molecular biology and immunology. It is a very simple method for amplifying nucleic acids in vitro. During the last two years PCR has mostly been used on investigations of oncogenes, tumor associated translocations, retroviral structures, HLA polymorphism and lymphokines. Because this method will make it possible to identify previously unknown structures it is particulary suitable for diagnosis in the broad field of degenerative and inflammatory diseases of the nervous system. Here we have two problems: a small amount of material and the necessity of rapid diagnostic procedures in inflammatory infections. PCR seems to be the technique which could overcome these difficulties.

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Editorial
Springer
Año de publicación
1991
Idioma
Inglés
ISBN 10 
3540529349
ISBN 13 
9783540529347
Encuadernación
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Número de edición
1
Número de páginas
272
Contacto del fabricante
no disponible
Persona responsable
CMN Printpool Inh. Mathis Neumann
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Hamburg
Alemania

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9780387529349: Pcr Topics: Usage of Polymerase Chain Reaction in Genetic and Infectious Diseases

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ISBN 10:  0387529349 ISBN 13:  9780387529349
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