This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
"Sobre este título" puede pertenecer a otra edición de este libro.
Librería: About Books, Henderson, NV, Estados Unidos de America
Hardcover. Condición: Very Good condition. NOT a library discard Ilustrador. First Edition. New York: Humana Press, 2019. Very Good condition but for notes on the front endpapers, and notes and a small soiled spot on the next two pages. All other pages are clean and unmarked with NO underlining. NO highlighting. NO margin notes. With many illustrations, some in color. Molecular diagrams. Tables. Bibliographical references. Index. Volume # 1961 in the publisher's Methods in Molecular Biology series. For no apparent reason, someone has drawn a harmless circle on the front cover (2 inches in diameter). From the rear cover: "This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field.". First Edition. Hardcover. Very Good condition/No dust jacket, as issued. Illus. by NOT a library discard. xi, 362pp. Great Packaging, Fast Shipping. Nº de ref. del artículo: 027944
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Librería: Brook Bookstore On Demand, Napoli, NA, Italia
Condición: new. Questo è un articolo print on demand. Nº de ref. del artículo: 7e75033207b77ea378bef9ce871db85c
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Librería: Basi6 International, Irving, TX, Estados Unidos de America
Condición: Brand New. New. US edition. Print on demand title. Delivery takes 20-25 days. Excellent Customer Service. Nº de ref. del artículo: POD-251989
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Librería: moluna, Greven, Alemania
Gebunden. Condición: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge techniquesProvides step-by-step detail essential for reproducible resultsContains key implementation advice from the expertsIncludes cutting-edge techniquesProvides step. Nº de ref. del artículo: 257581013
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Librería: GreatBookPricesUK, Woodford Green, Reino Unido
Condición: New. Nº de ref. del artículo: 34938246-n
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Librería: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Alemania
Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 376 pp. Englisch. Nº de ref. del artículo: 9781493991693
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Librería: Ria Christie Collections, Uxbridge, Reino Unido
Condición: New. In. Nº de ref. del artículo: ria9781493991693_new
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Librería: GreatBookPrices, Columbia, MD, Estados Unidos de America
Condición: New. Nº de ref. del artículo: 34938246-n
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Librería: GreatBookPrices, Columbia, MD, Estados Unidos de America
Condición: As New. Unread book in perfect condition. Nº de ref. del artículo: 34938246
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Librería: preigu, Osnabrück, Alemania
Buch. Condición: Neu. CRISPR Gene Editing | Methods and Protocols | Yonglun Luo | Buch | xi | Englisch | 2019 | Humana | EAN 9781493991693 | Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu Print on Demand. Nº de ref. del artículo: 115045499
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