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CRISPR Gene Editing: Methods and Protocols: 1961 (Methods in Molecular Biology, 1961) - Tapa dura

 
9781493991693: CRISPR Gene Editing: Methods and Protocols: 1961 (Methods in Molecular Biology, 1961)
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ISBN 10: 1493991698 ISBN 13: 9781493991693
Editorial: Humana, 2019

Sinopsis

This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field. 

Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.

"Sinopsis" puede pertenecer a otra edición de este libro.

De la contraportada

This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field. 

Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.

"Sobre este título" puede pertenecer a otra edición de este libro.

Editorial
Humana
Año de publicación
2019
Idioma
Inglés
ISBN 10 
1493991698
ISBN 13 
9781493991693
Encuadernación
Tapa dura
Número de edición
1
Número de páginas
376
Editor
Luo Yonglun
Contacto del fabricante
Springer Nature Customer Service Center GmbH
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