Sinopsis
This book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, and disease. Beginning with a section on in situ detection of RNA molecules using FISH techniques, the volume continues with parts exploring in vivo imaging of RNA transport and localization, imaging and analysis of RNA uptake and transport between cells, identification and analysis of RNA-binding proteins, guide RNAs in genome editing, as well as other specific analytical techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, RNA Tagging: Methods and Protocols serves as a vital reference for researchers looking to further the increasingly important research in RNA biology.
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Acerca del autor
Charlotte y Peter Fiell son dos autoridades en historia, teoría y crítica del diseño y han escrito más de sesenta libros sobre la materia, muchos de los cuales se han convertido en éxitos de ventas. También han impartido conferencias y cursos como profesores invitados, han comisariado exposiciones y asesorado a fabricantes, museos, salas de subastas y grandes coleccionistas privados de todo el mundo. Los Fiell han escrito numerosos libros para TASCHEN, entre los que se incluyen 1000 Chairs, Diseño del siglo XX, El diseño industrial de la A a la Z, Scandinavian Design y Diseño del siglo XXI.
De la contraportada
This book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, and disease. Beginning with a section on in situ detection of RNA molecules using FISH techniques, the volume continues with parts exploring in vivo imaging of RNA transport and localization, imaging and analysis of RNA uptake and transport between cells, identification and analysis of RNA-binding proteins, guide RNAs in genome editing, as well as other specific analytical techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, RNA Tagging: Methods and Protocols serves as a vital reference for researchers looking to further the increasingly important research in RNA biology.
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Condición: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge techniquesProvides step-by-step detail essential for reproducible resultsContains key implementation advice from the expertsIncludes cutting-edge techniquesProvides step. Nº de ref. del artículo: 356009686
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, and disease. Beginning with a section on in situ detection of RNA molecules using FISH techniques, the volume continues with parts exploring in vivo imaging of RNA transport and localization, imaging and analysis of RNA uptake and transport between cells, identification and analysis of RNA-binding proteins, guide RNAs in genome editing, as well as other specific analytical techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, RNA Tagging: Methods and Protocols serves as a vital reference for researchers looking to further the increasingly important research in RNA biology. 504 pp. Englisch. Nº de ref. del artículo: 9781071607114
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RNA Tagging | Methods and Protocols
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Buch. Condición: Neu. RNA Tagging | Methods and Protocols | Manfred Heinlein | Buch | xiv | Englisch | 2020 | Springer | EAN 9781071607114 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu Print on Demand. Nº de ref. del artículo: 118069955
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RNA Tagging: Methods and Protocols (Methods in Molecular Biology (2166))
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Buch. Condición: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Tagging and Application of RNA Probes for Sequence-Specific Visualization of RNAs by Fluorescent In Situ Hybridization.- Quantitative Fluorescence In Situ Hybridization Detection of Plant mRNAs with Single-Molecule Resolution.- Visualization of Endoplasmic Reticulum-Associated mRNA in Mammalian Cells.- Simultaneous Detection of mRNA and Protein in S. cerevisiae by Single Molecule FISH and Immunofluorescence.- Development and Applications of Fluorogen/Light-Up RNA Aptamer Pairs for RNA Detection and More.- Visualization of Transiently Expressed mRNA in Plants Using MS2.- New Generations of MS2 Variants and MCP Fusions to Detect Single mRNAs in Living Eukaryotic Cells.- In Vivo Imaging of Mobile mRNAs in Plants Using MS2.- RNA Imaging with RNase-Inactivated Csy4 in Plants and Filamentous Fungi.- Utilizing Potato Virus X to Monitor RNA Movement.- A Protocol for Non-Biased Identification of RNAs Transferred between Heterologous Mammalian Cell Types Using RNA Tagging,Cell Sorting, and Sequencing.- Synthesizing Fluorescently Labeled dsRNAs and sRNAs to Visualize Fungal RNA Uptake.- Labeling of dsRNA for Fungal Uptake Detection Analysis.- Using RNA Affinity Purification Followed by Mass Spectrometry to Identify RNA-Binding Proteins (RBPs).- Plant Individual Nucleotide Resolution Crosslinking and Immunoprecipitation to Characterize RNA-Protein Complexes.- A Single-Molecule RNA Mobility Assay to Identify Proteins that Link RNAs to Molecular Motors.- Proximity-CLIP Provides a Snapshot of Protein-Occupied RNA Elements at Subcellular Resolution and Transcriptome-Wide Scale.- Double-Stranded RNA Pull-Down to Characterize Viral Replication Complexes in Plants.- CRISPR Guide RNA Design Guidelines for Efficient Genome Editing.- Live Cell CRISPR Imaging in Plant Cells with a Telomere-Specific Guide RNA.- Live-Cell Imaging of Genomic Loci Using CRISPR/Molecular Beacon Hybrid Systems.- Using RNA Tags for Multicolor Live Imaging of Chromatin Loci andTranscription in Drosophila Embryos.- Integrated Genome-Scale Analysis and Northern Blot Detection of Retrotransposon siRNAs across Plant Species.- Transfection of Small Non-Coding RNAs into Arabidopsis thaliana Protoplasts.- In Vivo Reporter System for miRNA-Mediated RNA Silencing.- TRAP-SEQ of Eukaryotic Translatomes Applied to the Detection of Polysome-Associated Long Non-Coding RNAs.- Posttranscriptional Suzuki-Miyaura Cross-Coupling Yields Labeled RNA for Conformational Analysis and Imaging.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 504 pp. Englisch. Nº de ref. del artículo: 9781071607114
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RNA Tagging: Methods and Protocols (Methods in Molecular Biology (2166))
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RNA Tagging : Methods and Protocols
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Springer US, Springer US, 2020
ISBN 10: 1071607111
ISBN 13: 9781071607114
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Buch. Condición: Neu. Druck auf Anfrage Neuware - Printed after ordering - This book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, and disease. Beginning with a section on in situ detection of RNA molecules using FISH techniques, the volume continues with parts exploring in vivo imaging of RNA transport and localization, imaging and analysis of RNA uptake and transport between cells, identification and analysis of RNA-binding proteins, guide RNAs in genome editing, as well as other specific analytical techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, RNA Tagging: Methods and Protocols serves as a vital reference for researchers looking to further the increasingly important research in RNA biology. Nº de ref. del artículo: 9781071607114
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