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Añadir al carritoCondición: New. 2023rd edition NO-PA16APR2015-KAP.
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Librería: Kennys Bookshop and Art Galleries Ltd., Galway, GY, Irlanda
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Añadir al carritoTaschenbuch. Condición: Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume presents a broad collection of state-of-the-art methods to study the biology of thermogenic fat using in vitro cell culture and animal models. Chapters guide the readers through protocols on differentiation of human pluripotent stem cells and murine adipocyte precursors; methods for measuring mitochondrial respiration, heat generation, brown fat activation, and effects on energy metabolism in mice; and techniques for AAV-mediated gene delivery, transplantation of adipose tissue, isolation of adipose tissue immune cells and extracellular vesicles, andmass spectrometry-based profiling of brown fat lipids. Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge,Thermogenic Fat: Methods and Protocols aims to be comprehensive guide for researchers in the field.
Publicado por Springer New York, Springer New York, 2018
ISBN 10: 1493983083 ISBN 13: 9781493983087
Idioma: Inglés
Librería: AHA-BUCH GmbH, Einbeck, Alemania
EUR 152,32
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Añadir al carritoTaschenbuch. Condición: Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume presents a collection of protocols that describe methodologies to study thermogenic fat biology from various angles. This book is divided into 2 parts. Part 1 focuses on establishing in vitro culture systems. The chapters in this section introduce techniques on how to isolate, culture, and differentiate primary fat cells from both laboratory mice and humans. This part also presents flow cytometry methods to isolate various subpopulations of precursors within the stromal vascular fraction of the adipose tissue, which contains both preadipocytes and immune cells. Part 2 introduces multiple means to genetically manipulate and evaluate brown and beige fat in vivo. The chapters in this section explore methods on bioenergetics analyses both in vitro and in vivo. They also cover how to evaluate thermogenic fat contents and activity in humans, how to culture these cells though interdisciplinary approaches, and how to use thermogenic fat cell lines to carry out drug screens. Writtenin the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Thermogenic Fat: Methods and Protocolsis a valuable resource for both experts and novices who are interested in learning assays and investigating brown and beige fat functions.
Librería: Kennys Bookshop and Art Galleries Ltd., Galway, GY, Irlanda
EUR 207,99
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Publicado por Springer New York, Springer New York, 2017
ISBN 10: 149396819X ISBN 13: 9781493968190
Idioma: Inglés
Librería: AHA-BUCH GmbH, Einbeck, Alemania
EUR 153,90
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Añadir al carritoBuch. Condición: Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume presents a collection of protocols that describe methodologies to study thermogenic fat biology from various angles. This book is divided into 2 parts. Part 1 focuses on establishing in vitro culture systems. The chapters in this section introduce techniques on how to isolate, culture, and differentiate primary fat cells from both laboratory mice and humans. This part also presents flow cytometry methods to isolate various subpopulations of precursors within the stromal vascular fraction of the adipose tissue, which contains both preadipocytes and immune cells. Part 2 introduces multiple means to genetically manipulate and evaluate brown and beige fat in vivo. The chapters in this section explore methods on bioenergetics analyses both in vitro and in vivo. They also cover how to evaluate thermogenic fat contents and activity in humans, how to culture these cells though interdisciplinary approaches, and how to use thermogenic fat cell lines to carry out drug screens. Writtenin the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Thermogenic Fat: Methods and Protocolsis a valuable resource for both experts and novices who are interested in learning assays and investigating brown and beige fat functions.
EUR 218,51
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Añadir al carritoPaperback. Condición: Brand New. reprint edition. 214 pages. 10.00x7.00x0.54 inches. In Stock.
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Añadir al carritoHardcover. Condición: Brand New. 214 pages. 10.25x7.25x0.75 inches. In Stock.
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EUR 231,17
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EUR 175,09
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Añadir al carritoBuch. Condición: Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume presents a broad collection of state-of-the-art methods to study the biology of thermogenic fat using in vitro cell culture and animal models. Chapters guide the readers through protocols on differentiation of human pluripotent stem cells and murine adipocyte precursors; methods for measuring mitochondrial respiration, heat generation, brown fat activation, and effects on energy metabolism in mice; and techniques for AAV-mediated gene delivery, transplantation of adipose tissue, isolation of adipose tissue immune cells and extracellular vesicles, andmass spectrometry-based profiling of brown fat lipids. Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge,Thermogenic Fat: Methods and Protocols aims to be comprehensive guide for researchers in the field.
Librería: Mispah books, Redhill, SURRE, Reino Unido
EUR 232,39
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Añadir al carritoHardcover. Condición: Brand New. 265 pages. 10.00x7.01x0.63 inches. In Stock.
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Librería: Mispah books, Redhill, SURRE, Reino Unido
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Librería: Biblios, Frankfurt am main, HESSE, Alemania
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Librería: Majestic Books, Hounslow, Reino Unido
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Librería: Biblios, Frankfurt am main, HESSE, Alemania
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Librería: Biblios, Frankfurt am main, HESSE, Alemania
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