Springer us okt 2025 (16 resultados)

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides readers with a useful and comprehensive resource of laboratory protocols for working with Pichia pastoris. The chapters in this book are organized into seven parts: introduction, bioinformatics, cell and systems biology, gene expression cassettes and vectors, Pichia pastoris expression strains, upstream bioprocessing, and downstream processing and product analysis. This book covers basic and newer laboratory methods, starting from the gene and genetic construct to mature (yet reasonable) amounts of a recombinant product being produced, purified, and characterized. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Pichia pastoris: Methods and Protocols is a valuable resource for both new and experienced researchers who want to learn more about this important and developing field. 682 pp. Englisch. …

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume discusses protocols for key laboratory techniques used to study Duchenne and Becker muscular dystrophies. The chapters in this book cover a wide array of topics such as biochemical assays, quantification of dystrophin and related proteins, and measurements of muscle physiology. The chapters also cover methods of DMD sequence analysis; processing and isolation of cells from muscle tissue; culture and study of patient-derived cells; and work with animal models of DMD. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and authoritative, Duchenne Muscular Dystrophy: Methods and Protocols is a valuable resource that will contribute to the standardization of practices in the study of dystrophin and dystrophin-related disease. 312 pp. Englisch. …

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details methods and protocols of extraction, separation, characterization, and quantification of phytochemicals. Chapters cover extraction techniques, for instance, solvent extraction and supercritical fluid extraction, UV-Vis spectroscopy, high-performance liquid chromatography (HPLC), gas chromatography (GC), and liquid chromatography (LC) coupled with mass spectrometry (MS), used to measure the concentration of bioactive compounds. Written in the format of theMethods and Protocols in Food Scienceseries, chapters list necessary materials and methods for readily reproducible protocols.Authoritative and cutting-edge, Phytochemicals: Extraction, Quantification, Isolation, and Characterization aims to be comprehensive guide for researchers in the field. 348 pp. Englisch.…

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume highlights the heterogeneity and plasticity of enteric glial cells and explores their contributions to both GI homeostasis and disease pathophysiology. Through an interdisciplinary approach, the book brings together expertise from neurobiology, immunology, physiology, pharmacology, and pathology to provide a comprehensive perspective on the enteric nervous system and its glial components. It explores electrophysiological techniques, the relationship between enteric glia and the gut microbiota, advances in immunohistochemistry, the influence of the gut-brain axis on neurodegenerative diseases and mood disorders, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Enteric Glial Cells: Methods and Protocols serves as an ideal guide for understanding enteric glia but also for stimulating new research avenues that could lead to breakthroughs in treating a range of gastrointestinal and neurological disorders. 170 pp. Englisch.…

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Ketamine: From Neurobiology to Emergency and Anesthesia Care, Volume 1 discusses the latest advances in neuroscientific, translational, and clinical research, as well as practical applications of the essential drug racemic ketamine and its enantiomers esketamine and arketamine. Volume I covers ketamine s basic pharmacology and biology, as well as clinical developments in neurophysiology and neuroimaging with an emphasis on topics of consciousness and neuropsychiatric disorders such as depression. Pharmacokinetic and pharmacodynamic specifics with ketamine and its various administration routes are presented. The book also deals with racemic and esketamine s significance in clinical care of (prehospital) emergencies and trauma. The importance of ketamine in various anesthesia scenarios is addressed, including maternal and pediatric anesthesia, multimodal regimen, opioid-free anesthesia, and approaches to increase the quality of perioperative recovery. Periprocedural sedation with ketamine is described and potential benefits of ketamine for patient safety in neurosurgical anesthesia and sedation discussed.ThisNeuromethodsvolume offers concise details and advice regarding ketamine therapy from top researchers and clinician scientists. These insights are key to success in research and, most importantly, to improving patient outcomes. Ketamine: From Neurobiology to Emergency and Anesthesia Care, Volume 1 is a comprehensive, up-to-date resource and essential reference for both researchers and clinicians. People all around the world are substantially interested in ketamine, and racemic and es- ketamine s applications in medicine are rapidly expanding.This book is complemented by Ketamine: Pain Management, Critical Care, Depression, and Resource-Poor Setting, Volume II. 636 pp. Englisch.…

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second volume details new and updated methods on biology of various cellular processes and agronomic traits and come up with better strategies to improve legume crops. Chapters guide readers through legume genomic resources, legume pangenome and organelle genome construction, transcriptome analysis, DNA methylation analysis, double-digest restriction site-associated DNA sequencing; target enrichment sequencing via probe capture, genomic selection and transformation methods; prediction of long non-coding RNAs and secondary structures, genome-wide mining of disease resistance gene analogs; genome editing, and bioactive compound and phosphoproteome analysis. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Legume Genomics: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers in the field. 312 pp. Englisch.…

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The aim of this volume is to provide food scientists, biotechnologists and biochemists with a compilation of protocols focused on the extraction of bioactive compounds from fungi. Across its 21 chapters, the volume explores three fundamental areas: the extraction, quantification, and identification of fungal bioactive compounds; the evaluation of their effects on health; and the development of novel functional foods. Written in the format of theMethods and Protocols in Food Scienceseries, chapters list necessary materials and methods for readily reproducible protocols.Authoritative and cutting-edge,Fungi aims to be comprehensive guide forresearchers in the field. 316 pp. Englisch.…

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume summarizes the state-of-the-art methodology for the functional characterization of phytochromes in vitro and in vivo. Chapters and divided into four parts covering, phytochrome discovery, spectroscopic techniques, x-ray crystallography, x-ray crystallography, and analyzing phytochrome function in vitro and in vivo. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive,Phytochromes: Methods and Protocolsaims to be a versatile laboratory bench and computational resource that is an essential reference. 356 pp. Englisch. …

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents a comprehensive collection of protocols and techniques commonly used in research on plant-biotic interactions. Chapters guide readers through general plant pathogen interactions, RNA interference (RNAi) in Okra, spot inoculation of begomoviruses, natural transmission, RNA viruses, enzyme-linked immunosorbent, reverse transcription polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (RT-LAMP), northern blot hybridization, and next-generation sequencing (NGS) for in-depth analysis of viral populations and mutation patterns. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Plant Biotic Stress: Methods and Protocols aims to be comprehensive guide for researchers in the field. 332 pp. Englisch.…

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume presents protocols centered on the sensing and signaling of ethylene, a plant hormone with a vital role in fruit ripening, seed germination, stress adaptation, and a wide array of developmental processes. Beginning with a section on ethylene detection and quantification techniques, including gas chromatography and analysis during hypoxia and pathogen interactions, the book continues by examining the role of ethylene in post-harvest biology and fruit ripening, and associated regulatory mechanisms, including promoter characterization and protein-protein interactions. Further sections explore bioinformatics workflows for unraveling mechanistic roles of ethylene at the cellular and whole-plant levels, as well as the regulatory capacity of ethylene in plant developmental processes, root system modifications, and adaptation to diverse biotic and abiotic stressors. Written for the highly successful Methods in Molecular Biology series, chapters offer readers a hands-on approach to investigate the myriad roles that ethylene plays in plant growth and development. Authoritative and practical, Ethylene Sensing and Signaling serves as a key resource for scientists seeking to employ reliable methodologies in understanding the central function of ethylene in plant biology. 263 pp. Englisch.…

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume focuses on translational models of pediatric brain cancers that are critical to understanding tumor formation, tumor progression, and response to treatment. These models are particularly important for drug screens and preclinical testing of novel treatment approaches involving chemotherapy, radiation, and immunotherapy. The chapters in this book cover a variety of the latest technologies used to investigate the molecular backbone of pediatric brain tumors. These new diagnostic tools include tumor characterization from liquid biopsy; capturing the local heterogeneity of a single tumor through spatial genomics; and deciphering the unique epigenetic traits of pediatric brain tumors by profiling tumor methylome and post-translational histone modifications. Some chapters also describe the latest experimental tools designed to explore new therapeutic strategies and optimize current treatments. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and thorough, Translational Research Methods for Pediatric Brain Tumors is a valuable resource for clinicians and researchers who want to learn more about experimental pediatric neuro-oncology and help guide the field toward a brighter future. 192 pp. Englisch.…

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition details the techniques involved in the biosafety assessment of probiotics to be used for human consumption. New and updated chapters guide readers through identification of antibiotic resistance and virulence genes using various databases, PCR techniques, in vitro cytotoxicity assays, cytolysins, and in vivo toxicity assays such as acute and chronic toxicity, virulence, toxigenicity and dermonecrotic effects. Written in the format of theMethods and Protocols in Food Scienceseries, chapters list necessary materials and methods for readily reproducible protocols.Authoritative and cutting-edge, Biosafety Assessment of Probiotic Potential, Second Eiditionaims to be comprehensive guide for researchers in the field. 272 pp. Englisch.…

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details practical protocols and theoretical foundations for analyzing major and minor food components. Chapters guide readers on diverse range of food constituents, including macronutrients, micronutrients, vitamins, pigments, and volatile compounds. Written in the format of the Methods and Protocols in Food Scienceseries, chapters list necessary materials and methods for readily reproducible protocols.Authoritative and cutting-edge,Food Chemistry: Basic Protocols aims to be comprehensive guide for researchers in the field. 435 pp. Englisch. …

Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the latest advancements in the field of RNA therapeutics, with a particular focus on the development of exon skipping and inclusion therapies as targeted treatments for genetic diseases, as well as the evaluation methods used post-treatment. The chapters in this book cover both established methods and novel techniques used my researchers working on developing therapies that have proven to be promising for several diseases, including Duchenne muscular dystrophy (DMD), spinal muscular atrophy (SMA), and other genetic disorders characterized by abnormal splicing events. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Splice Switching: Methods and Protocols is a valuable resource for researchers across various fields. This book will help in the advancement of basic RNA biology research and aid in accelerating therapeutic developments. 240 pp. Englisch. …

Buch. Condición: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume details methods and protocols of extraction, separation, characterization, and quantification of phytochemicals. Chapters cover extraction techniques, for instance, solvent extraction and supercritical fluid extraction, UV-Vis spectroscopy, high-performance liquid chromatography (HPLC), gas chromatography (GC), and liquid chromatography (LC) coupled with mass spectrometry (MS), used to measure the concentration of bioactive compounds. Written in the format of the Methods and Protocols in Food Science series, chapters list necessary materials and methods for readily reproducible protocols.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 348 pp. Englisch.…

Buch. Condición: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume details techniques involved in the study of endosymbionts. Chapters divided into four sections detail isolation, maintenance of diverse endosymbionts, classical microscopy, staining, PCR, FISH, flow cytometry, next-generation sequencing, endosymbionts, metabolites, and bacterial endosymbionts in insects. Step-by-step protocols are complemented by personal notes, precautions, and specifying the reagents to be used in each step to ensure the repeatability of the procedure across labs.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 284 pp. Englisch.…