Springer us jan 2020 (21 resultados)
Idioma: Inglés
Editorial: Springer US Jan 2020, 2020
Serie: Springer Protocols Handbooks, Libro 43 de 84. Libro 43 de 84 - Springer Protocols Handbooks
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Librería: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, AlemaniaBuchWeltWeit Ludwig Meier e.K.
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EUR 181,89
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Buch. Condición: Neu. Neuware -This volume details methods and protocols necessary to further the study of insect immunity. Chapters guide readers through up-to-date genomic and transcriptomic approaches, insect samples for proteomic analysis, hemocytes in Drosophila, cellular response in Lepidoptera, insect AMPs, manipulate the… composition of mosquito microbiota, viral infections in insects, infections by entomopathogenic nematodes, immune response following oral infections, and protocols to to monitor the effect of septic infections with human pathogens using B. mori as a model. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Immunity in Insects aims to ensure successful results in the further study of this vital field. 260 pp. Englisch.
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EUR 53,49
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This open access volume presents state-of-the-art inference methods in population genomics, focusing on data analysis based on rigorous statistical techniques. After introducing general concepts related to the biology of genomes and their…evolution, the book covers state-of-the-art methods for the analysis of genomes in populations,including demography inference, population structure analysis and detection of selection, using both model-based inference and simulation procedures. Last but not least, it offers an overview of the current knowledge acquired by applying such methods to a large variety of eukaryotic organisms.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, pointers to the relevant literature, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Statistical Population Genomicsaimsto promote and ensure successful applications of population genomic methods to an increasing number of model systems and biological questions. 484 pp. Englisch.
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EUR 160,49
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the latest techniques used by researchers to study DNA electrophoresis, with focus on various species including bacteria, yeasts, and mammalian cells. The chapters in this book cover topics such as two-dimensional gel…electrophoresis; DNA replication; pulsed-field gel electrophoresis; ChIP; and post-labeling/PAGE method for detection of DNA adducts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, DNA Electrophoresis: Methods and Protocols is a valuable resource for any researchers looking to learn more about this developing field. 240 pp. Englisch.
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EUR 160,49
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the latest metabolic flux analysis (MFA) techniques that cover the analysis of cellular, organ level, and whole-body metabolism. The chapters in this book discuss topics such as deutrium tracing; isotopologue fractions… using GC-TOF; non-targeted mass isotopolome analysis; large-scale profiling of cellular metabolic activities using deep 13C labeling medium; metastases in mice; SWATH; Exo-MFA; metabolic flux from time-course metabolomics; and thermodynamic approaches in flux analysis. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive,Metabolic Flux Analysis in Eukaryotic Cells: Methods and Protocolsis a valuable resource for both experts in MFA techniques and researchers getting involved in the role of quantitative studies to uncover the dysregulated pathways in human diseases. 384 pp. Englisch.
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EUR 160,49
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume includes in-depth, hands-on protocols for detecting various developmental defects in Drosophila. It provides cutting-edge methods for maintaining Drosophila under laboratory conditions to perform various experiments, and for di…ssecting various imaginal discs of the larvae. Further, biochemical protocols for estimating the levels of different metabolites, reactive oxygen species, and fat-sensitive pathways are discussed, and various staining and behavior techniques for fat detection are provided. The book explains how various fluorescent dyes and the comet assay can be used to identify DNA damage, and elaborates on the analysis of the eye, antennae, imaginal disc, gut and muscle damage under bright field and fluorescent microscopes. It covers the analysis of hemolymph using Giemsa staining; determining the functionality of channel proteins, eye and mechanosensory organs in adults and larvae using various behavioral assays; and metal detection and structural analysis for various tissues using SEM. In closing, it addresses the analysis of Drosophila's eye and head via paraffin section; measurement of reactive oxygen species from various tissues via FACS; and the CRISPER technique for gene editing and analysis of micro RNA mutations. 276 pp. Englisch.
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EUR 171,19
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 312 pp. Englisch.
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EUR 171,19
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition provides techniques used to study of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. Chapters detail next-generation sequencing technologies, data analysis, protocols… on analysis and visualization of single-cell RNA-Seq data, analysis of 3D chromatin architecture, interpretation of large-scale interaction networks, transcriptional networks in embryonic and adult stem cells, derivation of stem cells, and transcriptional programs that promote reprogramming, transdifferentiation, and cancer formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Stem Cell Transcriptional Network: Methods and Protocols, Second Edition aims to provide a key resource for biologists seeking to interrogate these vital networks. 320 pp. Englisch.
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EUR 181,89
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches that are used to quantify immune mediators and analyze their function and biological activity i…n cancer cells and tissues. Beginning with a section on the detection of immune mediators in samples, the volume continues with sections covering cytokine bioassays, the expression and regulation of immune mediators in cancer cells, and methods to navigate the enormous datasets created by modern DNA and RNA sequencing and proteomic technology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and wide-ranging, Immune Mediators in Cancer: Methods and Protocols serves as a valuable resource for biochemists, molecular biologists, cancer biologists, and immunologists, as well as for physician-scientists working in the field of immunology and cancer research. 388 pp. Englisch.
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EUR 181,89
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book serves as a guide for identifying and applying commonly used cell-based translational assays as well as for assessing the therapeutic potential of new immuno-oncology therapeutics and advancing their mechanism of action. The deta…iled chapters within will provide readers with a baseline understanding of the pros and cons as well as key considerations for applying assays that are more reflective of the human immune-tumor microenvironment in order to increase their translatability into the clinic. Written for the Methods in Pharmacology and Toxicology series, the contents of this volume include the kind of specifics and real-world implementation advice to ensure success in the lab. Authoritative and practical, Immuno-Oncology: Cellular and Translational Approaches aims to aid researchers working on biotechnology and pharmaceutical efforts to search for the next generation of safer and more effective cancer immunotherapeutics. 276 pp. Englisch.
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EUR 192,59
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides current and new advanced methods and protocols to study T cells. Chapters guide readers through T cell diversity using mass cytometry, analyzing T cells from single cell level, CRISPR/Cas9 techniques to study the T cel…l activation, techniques to study subsets of Tcell's, procedures to study artificial antigen presentosomes for T cell activation, techniques to study the T cell development, two-photon microscopy, and MAIT cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, T-Cell Receptor Signaling: Methods and Protocols aims to provide a wide range of approaches and be an invaluable resource for present and future generations of T cell researchers. 308 pp. Englisch.
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EUR 192,59
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Twenty-five years ago, micro- and nanotechnology were barely in their infancy. Today, technological advancements at the lower end of the nanoscale have radically changed the field of medicine, allowing for innovative and more personalized…approaches to numerous pathologies.The initial cascade of advancements was triggered by the herculean efforts of a few pioneers in the field. Dr. Mauro Ferrari is one such visionary; his work over the past two decades has set the stage for medical innovation through nanotechnology.Written by world-renowned experts in the field, this book pays tribute to Dr. Mauro Ferrari's vast contribution to the field of nanotechnology and nanomedicine. It covers the multifaceted and fundamental aspects of biomedical research, including technological innovation, ethics, patient advocacy, and clinical translation, all areas where Dr. Ferrari's contribution has been and continue to be fundamental. Additionally, it presents recent advances in micro- and nanotechnologies.Chapter 2 and Chapters 9 through 27 were originally published in Biomedical Microdevices in the Topical Collection 'Biomedical Micro-Nanotechnologies toward Translation' 352 pp. Englisch.
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EUR 160,49
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Buch. Condición: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume explores the latest metabolic flux analysis (MFA) techniques that cover the analysis of cellular, organ level, and whole-body metabolism. The chapters in this book discuss topics such as deutrium tracing; isotopologue fractions usi…ng GC-TOF; non-targeted mass isotopolome analysis; large-scale profiling of cellular metabolic activities using deep 13C labeling medium; metastases in mice; SWATH; Exo-MFA; metabolic flux from time-course metabolomics; and thermodynamic approaches in flux analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 384 pp. Englisch.
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EUR 213,99
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume looks at the latest research techniques to study the interaction of visual spatial learning and attention guidance with behavioral, psychophysiological, and imaging methods. Part One (behavioral methods) focuses on different pa…radigms of visual search like visual foraging and contextual cueing, and also methods like feature distribution analysis and search in virtual reality. Part Two (psychophysiological methods) integrates innovative uses of classical potential changes like the CDA and N2pc, with multivariate analysis methods and multi-method designs. Part Three (functional imaging) covers lesion-behavior mapping, retinotopic and grid cell mapping methods for human fMRI, as well as functional registration by hyperalignment and simultaneous eye-tracking and fMRI. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and comprehensive, Spatial Learning and Attention Guidance is a valuable resource for all researchers and scientists who are interested in learning more about the relationship between attention and memory. 328 pp. Englisch.
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EUR 213,99
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume focuses on computational modeling of the ecotoxicity of chemicals and presents applications of quantitative structure-activity relationship models (QSARs) in the predictive toxicology field in a regulatory context. The extensiv…e book covers a variety of protocols for descriptor computation, data curation, feature selection, learning algorithms, validation of models, applicability domain assessment, confidence estimation for predictions, and much more, as well as case studies and literature reviews on a number of hot topics. Written for the Methods in Pharmacology and Toxicology series, chapters include the kind of practical advice that is essential for researchers everywhere.Authoritative and comprehensive, Ecotoxicological QSARs is an ideal source to update readers in the field with current practices and introduce to them new developments and should therefore be very useful for researchers in academia, industries, and regulatory bodies. 852 pp. Englisch.
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EUR 192,59
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Buch. Condición: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Written by world-renowned experts in the field, this book pays tribute to Dr. Mauro Ferrari¿s vast contribution to the field of nanotechnology and nanomedicine. It covers the multifaceted and fundamental aspects of biomedical research, includi…ng technological innovation, ethics, patient advocacy, and clinical translation, all areas where Dr. Ferrari¿s contribution has been and continue to be fundamental. Additionally,it presents recent advances in micro- and nanotechnologies.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 352 pp. Englisch.
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EUR 235,39
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This fully revised book includes new and improved protocols to analyze toxicity at the DNA, RNA, and protein levels using genomic, epigenomic, transcriptomic, and proteomic approaches. Sections of the book address key subjects such as bioi…nformatics and biostatistics, toxicant-induced lung inflammation and tumors, analysis of protein and gene expression and silencing, gene promoter methylation, detection of chromosomal and genome-wide damage, analysis of DNA-adducts and surrogate gene mutations, detection and characterization of cancer gene mutation, and many other areas of study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and up-to-date, Molecular Toxicology Protocols, Third Edition will prove to play an important role in generating a better understanding of the field of toxicology and its sub-disciplines. 592 pp. Englisch.
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EUR 246,09
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book provides a comprehensive guide to scientists, engineers, and students that employ metabolomics in their work, with an emphasis on the understanding and interpretation of the data. Chapters guide readers through common tools for d…ata processing, using database resources, major techniques in data analysis, and integration with other data types and specific scientific domains.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, practical guidance of methods and techniques, useful web supplements, and connect the steps from experimental metabolomics to scientific discoveries.Authoritative and cutting-edge,Computational Methods and Data Analysis for Metabolomicsto ensure successful results in the further study of this vital field. 504 pp. Englisch.
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EUR 213,99
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Buch. Condición: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume focuses on computational modeling of the ecotoxicity of chemicals and presents applications of quantitative structure¿activity relationship models (QSARs) in the predictive toxicology field in a regulatory context. The extensive bo…ok covers a variety of protocols for descriptor computation, data curation, feature selection, learning algorithms, validation of models, applicability domain assessment, confidence estimation for predictions, and much more, as well as case studies and literature reviews on a number of hot topics. Written for the Methods in Pharmacology and Toxicology series, chapters include the kind of practical advice that is essential for researchers everywhere.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 852 pp. Englisch.
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EUR 320,99
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring way…s to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field. 516 pp. Englisch.
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EUR 695,49
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Buch. Condición: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This new book addresses the status of the field of System Dynamics 60+ years after its inception. It presents state-of-the-art expositions by leading authorities in either a facet of the theory and methodology of the subject or its application… in a specific domain. Exhibiting greater reach and authority than would be possible in a conventional authored textbook, the volume includes nine chapters covering methodological aspects, and 14 on various contemporary applications.Emerging from the System Dynamics section of the Encyclopedia of Complexity & Systems Science, First Edition (2009), the book features brand new chapters covering project management, workforce modelling, applications in defense, operations management, engineering of strategy, the roots of model validation, as well as many considerably enhanced versions of existing chapters. Together, the chapters reveal a remarkable landscape of theory and practice, and how System Dynamics can contribute criticalpolicy insights to a broad audience of students and professionals across many fields of study.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 556 pp. Englisch.
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EUR 695,49
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Buch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This new book addresses the status of the field of System Dynamics 60+ years after its inception. It presents state-of-the-art expositions by leading authorities in either a facet of the theory and methodology of the subject or its applica…tion in a specific domain. Exhibiting greater reach and authority than would be possible in a conventional authored textbook, the volume includes nine chapters covering methodological aspects, and 14 on various contemporary applications.Emerging from the System Dynamics section of the Encyclopedia of Complexity & Systems Science, First Edition (2009), the book features brand new chapters covering project management, workforce modelling, applications in defense, operations management, engineering of strategy, the roots of model validation, as well as many considerably enhanced versions of existing chapters. Together, the chapters reveal a remarkable landscape of theory and practice, and how System Dynamics can contribute criticalpolicy insights to a broad audience of students and professionals across many fields of study. 556 pp. Englisch.
