springer new york jun 2018 (331 resultados)

Taschenbuch. Condición: Neu. Neuware -Current worldwide trends are not sustainable. The Club of Rome¿s warnings published in the book Limits to Growth are still valid. Remedies that are acceptable for the great majority tend to make things worse. We seem to be in a philosophical crisis. Pope Francis says it clearly: our common home is in deadly danger. Analyzing the philosophical crisis, the book comes to the conclusion that the world may need a ¿new enlightenment¿; one that is not based solely on doctrine, but instead addresses a balance between humans and nature, as well as a balance between markets and the state, and the short versus long term. To do this we need to leave behind working in ¿silos¿ in favor of a more systemic approach that will require us to rethink the organization of science and education.However, we have to act now; the world cannot wait until 7.6 billion people have struggled to reach a new enlightenment.This book is full of optimistic case studies and policy proposals that will lead us back to a trajectory of sustainability. But it is also necessary to address the taboo topic of population increase. Countries with a stable population fare immensely better than those with continued increase.Finally, we are presenting an optimistic book from the Club of Rome.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 236 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This volume presents techniques and recently developments in extracellular recording. The chapters in this book explore topics such as mouse, rat and zebrafish models; extracellular recordings on human cognition; single-unit recordings and multi-electrode arrays; and recordings in perinatal and early developing mice. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and authoritative, Extracellular Recording Approaches is a valuable resource that provides readers with up-to-date applications and methods to study brain regions involved in sensory, motor, and cognitive functions.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 312 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This volume is the result of an explosion ofmolecular-based research on Cilia, which began with the discovery of theuniversality of intraflagellar transport (IFT) and ciliary genomics/proteomics.The chapters in this book cover topics such as: high resolution imaging andfunctional characterization of sensory and primary cilia in mammalian cells andzebrafish, methods to study ciliary-mediated chemoresponse in Parameciumand methods to study centrosomes and cilia in C. elegans and Drosophila.Written in the highly successful Methods in Molecular Biology series formatchapters include introduction to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Practical and cutting-edge, Cilia:Methods and Protocols is broad and covers motile, sensory, and primarycells. It is a valuable resource to anyone interested in entering the field ofciliary biology using model organisms, including flagellate algae, ciliatesplanaria, nematodes, insects, zebrafish, and mammalian cells.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 276 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This volume presents relevantbackground information to understanding the molecular basis governingunconventional protein secretion (UPS), and in particular explores the latesttechniques and protocols that have been successfully applied for the study ofthis topic. Detailed chapters include an overview of conventional andunconventional secretory pathways along with multidisciplinary approaches andmethods used for UPS analysis in different organisms. Written in the highly successful Methods inMolecular Biology series format,chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-stepreadily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.Authoritative and cutting-edge, UnconventionalProtein Secretion: Methods and Protocols will beusefulfor all interested in the secretory pathway field as well as applications incell biology, cell development, biomedical research, and healthcare.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 288 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This fully updated and revised second edition address specifically some of the significant advances made in our understanding of the pathophysiology of penile cancer including discussion of the suspected role of the human papilloma virus in penile carcinogenesis. Similarly, the molecular pathways implicated in precancerous and penile cancer will be discussed as these are the present targets of novel therapeutic approaches to this disease. The text details the recent national and international diagnostic and treatment guidelines that have been developed by both the National Comprehensive Cancer Network and the European Urological Association establishing evidence based standards upon which the care of penile cancer should be based. The text also covers significant advances made in the diagnostic and therapeutic approach to both primary tumors and inguinal nodal metastases resulting from penile cancer such as penile sparing surgery and minimally invasive inguinal lymph node surgery. Thetext will similarly highlights the improvement in cancer specific outcome in the management of loco-regionally advanced (N2/3) penile cancer managed by upfront systemic chemotherapy followed by consolidative surgery in appropriately selected patients based on recent prospective phase II data. It also covers the role of radiotherapy in the management of penile cancer for both primary tumors and/or at sites of regional or distant metastases. Finally, some of the novel systemic approaches being devised in the management of advanced penile cancer along with some upcoming international trials soon to be opened which likely will re-define the present therapeutic approaches to penile cancer are discussed.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 164 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This book provides a practical and self-contained overview of the Gene Ontology (GO), the leading project to organize biological knowledge on genes and their products across genomic resources. Written for biologists and bioinformaticians, it covers the state-of-the-art of how GO annotations are made, how they are evaluated, and what sort of analyses can and cannot be done with the GO. In the spirit of the Methods in Molecular Biology book series, there is an emphasis throughout the chapters on providing practical guidance and troubleshooting advice.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 320 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -The revised second edition of this textbook provides the reader with a solid foundation in probability theory and statistics as applied to the physical sciences, engineering and related fields. It covers a broad range of numerical and analytical methods that are essential for the correct analysis of scientific data, including probability theory, distribution functions of statistics, fits to two-dimensional data and parameter estimation, Monte Carlo methods and Markov chains.Features new to this edition include:¿ a discussion of statistical techniques employed in business science, such as multiple regression analysis of multivariate datasets.¿ a new chapter on the various measures of the mean including logarithmic averages.¿ new chapters on systematic errors and intrinsic scatter, and on the fitting of data with bivariate errors.¿ a new case study and additional worked examples.¿ mathematical derivations and theoretical background material have been appropriately marked, to improve the readability of the text.¿ end-of-chapter summary boxes, for easy reference.As in the first edition, the main pedagogical method is a theory-then-application approach, where emphasis is placed first on a sound understanding of the underlying theory of a topic, which becomes the basis for an efficient and practical application of the material. The level is appropriate for undergraduates and beginning graduate students, and as a reference for the experienced researcher. Basic calculus is used in some of the derivations, and no previous background in probability and statistics is required. The book includes many numerical tables of data, as well as exercises and examples to aid the readers' understanding of the topic.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 336 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This volume provides properties, biological function, methodsand approaches for manipulating nAChRs in different organisms. NicotinicAcetylcholine Receptor Technologies guides readers through moleculartechniques and behavioral tests used to investigate nicotinic drugschronoamperometry, emerging technologies and methods for the analysis ofnAChRs, single nucleotide polymorphism (SNP), fluorescence techniques, spectralconfocal, allosteric modulators of a7-nAChRs, and a comprehensive evolutionalrelation for most nAChR subunits, in both vertebrate and invertebrate species.Written for the popular Neuromethods series, chapters include thekind of detail and key implementation advice that ensures successful results inthe laboratory.Authoritative and practical, Nicotinic AcetylcholineReceptor Technologies aims to ensure successful results in thefurther study of this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 272 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This textbook consists primarily of notes by Iain Finnie who taught a popular course on fracture mechanics at the University of California at Berkeley. It presents a comprehensive and detailed exposition of fracture, the fundamentals of fracture mechanics and procedures for the safe design of engineering components made from metal alloys, brittle materials like glasses and ceramics, and composites. Interesting and practical problems are listed at the end of most chapters to give the student practice in applying the theory. A solutions manual is provided to the instructor. The text presents a unified perspective of fracture with a strong fundamental foundation and practical applications. In addition to its role as a text, this reference would be invaluable for the practicing engineer who is involved in the design and evaluation of components that are fracture critical.This book also:Presents details of derivations of the basic equations of fracture mechanics and the historical context of the development of fracture theory and methodologyTreats linear and nonlinear fracture mechanics methodologies beginning with a review of the basic equations of solid mechanics followed by solutions useful in fracture predictionIllustrates the basis of linear elastic fracture mechanics (LEFM), practical applications of LEFM in the design of fracture-tolerant structural componentsOffers interesting, practical, classroom proven problems at the end of most chaptersIncludes instructor's solutions manualSpringer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 268 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This guide provides a comprehensive review of the full spectrum of hypertension in chronic kidney disease (CKD). Targeted towards the busy practitioner, the focus of this volume is on various therapies and how to lower blood pressure through lifestyle changes. Specialist patient populations and hypertension and causes of hypertension are also covered in detail. Clinically-focused and authoritative, this resource offers a rationalized approach to diagnosing and treating hypertension in CKD.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 360 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This second edition provides updated and expanded chapters covering a broad sampling of useful and current methods in the rapidly developing and expanding field of bioinformatics. Bioinformatics, Volume I: Data, Sequence Analysis, and Evolution, Second Edition is comprised of three sections: Data and Databases, Sequence Analysis, and Phylogenetics and Evolution. The first section details bioinformatics methodologies in the generation of sequence and structural data and its organization into conceptual categories, and databases to facilitate further analyses. The Sequence Analysis section describes the fundamental methodologies for processing the sequences of biological molecules: techniques that are used in almost every pipeline of bioinformatics analysis, particularly in the preliminary stages of such pipelines. Last but not least, the phylogenetics and evolution section deals with methodologies that compare biological sequences for the purpose of understanding how they evolved. As a volume in the highly successful Methods in Molecular Biology series, chapters feature the kind of detail and expert implementation advice to ensure positive results.Comprehensive and practical, Bioinformatics, Volume I: Data, Sequence Analysis, and Evolution, Second Edition is an essential resource for graduate students, early career researchers, and others who are in the process of integrating new bioinformatics methods into their research.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 504 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This volume presents a collection of tools currently used for the characterization of rust, the host plant wheat, and their interactions. This book is divided into five parts: Parts I and II discuss advanced techniques for characterizing rust pathogens in rust surveillance, genotyping, and molecular pathogenicity; Part III describes protocols for genetic analysis of rust resistance; Part IV covers methods on rust resistance gene cloning; and Part V talks about the isolation and screening of bacterial endophytes as biocontrol agents for rust disease management. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Wheat Rust Disease: Methods and Protocols is a valuable resource for both established and novel wheat rust researchers and also the plant science and microbial research community.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 308 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and TaqMan-based assays; in situ analysis of variants in single RNA molecules; the MassARRAY system and Molecular Inversion Probes; Pulsed Field Gel Electrophoresis, Paralogue Ratio Test, and Multiplex Ligation-Dependent Probe Amplification; long-range PCR combined with PacBio sequencing; Targeted Locus Amplification; Multilocus Sequence Typing and rapid SNP detection with pyrosequencing; and genotyping-by-sequencing for plant analysis. Finally, the volume concludes with a summary of pertinent points to describe genetic variation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and practical, Genotyping: Methods and Protocols is a valuable resource for anyone interested in learning more about the diverse field of genotyping.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 264 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. The methodologies include using Wnt modulating chemicals in engineering tissues from induced pluripotent and embryonic stem cells, monitoring Wnt transcriptional responses in diverse tissues such as bone and skin, and using specific biochemical markers of Wnt signaling to either screen molecular libraries or evaluate novel reagents. These protocols also leverage unique experimental strengths from five different model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 212 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This volume presents methods and techniques to studyoogenesis in a broad range of organisms, from plants to mammals. Oogenesis: Methods and Protocols guidesreaders through protocols on models ofdevelopmental biology, oogenesis in plants, worms, fruit fliesmosquitos, butterflies, starfish, zebrafish, frog, chicken and mouse. Written in the highly successful Methodsin Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-stepreadily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.Authoritative and cutting-edge, Oogenesis:Methods and Protocols aims to ensure successful results in the furtherstudy of this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 288 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This volume provides an up-to-date compilationof current methodological approaches utilized for the exploration of nucleolarstructure and function. Chapters covera diversity of protocols that include imaging of the nucleolus, analysis ofribosomal RNA transcription and processing, and genomics and proteomics of thenucleolus. Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, The Nucleolus: Methods and Protocols provides scientists with a reliable practicalhandbook to facilitate the investigation of this nuclear compartment at theadvanced level.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 292 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This volume highlights the molecular and cellular methods used in studying Chronic Myeloid Leukimia (CML) pathogenesis and stem cell biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Chronic Myeloid Leukemia: Methods and Protocols aims to ensure successful results in the further study of this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 272 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This second edition provides updated and new chapters on selected genetic, molecular, biochemical, and cell biological techniques. Chapter¿s guide readers through methods and principles on primordial germ cells and germline stem cells, however many of these principles can be applied to different types of adult stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Germline Stem Cells: Second Edition aims to present the new findings and techniques that have developed since the first edition.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 244 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This volume covers arange of methods used in plant cytogenetics, beginning with basic analysis ofchromosomes and visualizing gene locations, to manipulating and dissectingchromosomes, and then focusing on less understood features of chromosomes suchas recombination initiation sites and epigenomic marks. The methods describedin Plant Cytogenetics: Methods and Protocols build on each other andprovide, those new to the field, with a comprehensive platform to support theirresearch endeavours, while also introducing advanced techniques to experiencedresearchers. Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Cuttingedge and thorough, Plant Cytogenetics: Methods and Protocols, is avaluable resource for anyone who is interested in the diverse and wonderfullycomplex field of cytogenetics.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 212 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This volume focuses and describes tools, assays, and techniques used to enhance the understanding of the role of auxins and cytokinins. The chapters in this book cover topics such as: microbial manipulation of auxin and cytokinins in plants; interplay between auxin and cytokinin and its impact on MAPK; H2O2 production in Arabidopsis leaves; crosstalk between jasmonate and auxin in plant stress responses of roots; and high-throughput protoplast trans-activation (PTA) screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Auxins and Cytokinins in Plant Biology: Methods and Protocols is a valuable resource to plant scientists, graduate and under graduate students in addressing their biological questions relevant to the functional implications of auxin and cytokinins.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 216 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This volume provides a comprehensive overview of the experimental and computational methodologies used to study the function of long non-coding RNA (ncRNAs) expressed from enhancers. Chapter detail both wet-lab and dry-lab techniques and annotating long ncRNAs and exploring transcription by assessing where transcription starts and generally how it occurs.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Enhancer RNAs: Methods and Protocols aims to ensure successful results in this rapidly developing field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 268 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -Small molecule microarrays (SMM) were introduced in 1999 and within just a short span of time, have established themselves as a vibrant next generation platform for high-throughput screening. Small Molecule Microarrays: Methods and Protocols consolidates a significant collection of examples, which serve to guide researchers toward ways in which SMM technology may be effectively deployed for multiplexed screening, drug discovery and ligand identification. The volume is organized into three sections covering microarray fabrication, imaging modes, and discovery approaches. The chapters cover a range of small molecule library types, ranging from synthetic combinatorial libraries to peptide and carbohydrate libraries, with lessons and techniques that can be widely applied. Written in the highly successful Methods in Molecular Biology series, format, chapters include introductions of their respective topics, lists of the requisite materials and reagents, step-by-step and readily reproducible laboratory protocols as well as vital tips on troubleshooting and advice on avoiding known pitfalls.Authoritative and cutting-edge, Small Molecule Microarrays: Methods and Protocols provides meticulous insights into the successful applications of SMM, by experts in the field. Building on this collection of knowledge, the volume seeks to inspire a future generation of microarray practitioners to propel this significant technology to even greater heightsHumana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 272 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This volume examines the structure and dynamics of the bacterial flagellum using bacterial genetics, molecular biology, biochemistry, structural biology, biophysics, cell biology, and molecular dynamics simulation. The chapters are divided into 4 parts: Part I describes flagellar type III protein exports, assembly, and gene regulation in S. enterica; Part II explains how to isolate the flagella from the bacterial cell bodies, and further explains how to conduct high-resolution structural and functional analyses of the flagellar motor; Part III talks about how to measure flagellar motor rotation over a wide range of external load, how to measure ion motive force across the cytoplasmic membrane, and how to measure dynamic properties of the flagellar motor proteins by fluorescence microscopy with single molecule precision; and Part IV explores the structure and function of Spirochetal, Vibrio, Shewanella, and Magnetococcus flagellar motors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, The Bacterial Flagellum: Methods and Protocols aims to provide valuable and vital research to aid in the investigation of the bacterial flagellum resulting from various bacterial species.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 340 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This volume aims to provide a range of methods and protocols for studying tumor angiogenesis in vitro and in vivo to reflect advances in the field. The chapters in this book cover topics such as: morphological aspects of tumor angiogenesis, aortic ring assay and its use for the study of tumor angiogenesis, ex vivo tissue culture model for anti-angiogenic drug testing, transgenic zebrafish, orthotopic models of ovarian cancer, and uncovering metabolic effects of anti-angiogenic therapy in tumors by induced metabolic bioluminescence imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge and thorough, Tumor Angiogenesis Assays: Methods and Protocols is a valuable resource for anyone interested in tumor angiogenesis assay research.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 208 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -Thisvolume summarizes current techniques; ranging from protocols for virus growthisolation, quantification and generation of recombinant RSV virus to proceduresfor the efficient characterization of the host immune response to RSVinfection. These techniques are used in numerous laboratories around the worldand are the building blocks that support the majority of RSV virus research. Written in the highly successful Methods in MolecularBiology series format, chapters include introductions to their respectivetopics, lists of the necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls.Authoritative and cutting-edge, Humana Respiratory Syncytial Virus:Methods and Protocols aims to ensure successful resultsin the further study of this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 260 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This volume reviews and summarizes some of A. I. McLeod's significant contributions to time series analysis. It also contains original contributions to the field and to related areas by participants of the festschrift held in June 2014 and friends of Dr. McLeod. Covering a diverse range of state-of-the-art topics, this volume well balances applied and theoretical research across fourteen contributions by experts in the field. It will be of interest to researchers and practitioners in time series, econometricians, and graduate students in time series or econometrics, as well as environmental statisticians, data scientists, statisticians interested in graphical models, and researchers in quantitative risk management.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 304 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -High-throughput RNAi screening remains one of the most widely used technologies to perform target identification and validation studies in an unbiased manner. These assays are equally important for research and development across academic, biotech, and pharmaceutical industries. The success of these screening efforts is dependent on robust methodologies to perform these screens. In High-Throughput RNAi Screening: Methods and Protocols, expert researchers in the field share protocols and methods for performing high-throughput RNAi (HT-RNAi) screens. These include the use of various RNAi platforms and delivery methods in mammalian and non-mammalian systems, whole organism and cell models, and various applications, such as drug sensitizer identification. Finally, the book examines the latest advancements in the fields of assay development, library screening, data analysis, and hit selection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, High-Throughput RNAi Screening: Methods and Protocols provides a comprehensive source of protocols and other necessary information to make robust and successful assays possible for all who wish to apply HT-RNAi in their research.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 276 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This second edition volume provides an overview of some of the types of nanostructures commonly used in nanobiomedicine. The chapters in this book discuss practical information on the synthesis and characterization of a variety of solution-phase and surface-bound nanomaterials, with examples of how they can be used in sensing, imaging, and therapeutics. Specific topics include the synthesis and characterization of molecule and biomolecule-functionalized nanoconjugates with gold, iron oxide, or polymeric cores; the development of biosensing, imaging, and therapeutic applications of multicomponent/multifunctional nanostructures; and the application of flow cytometry in nanobiomedicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 360 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This second edition provides 21 new chapters on methods used in laboratories for investigating the physiology and molecular genetics of the pathogen Clostridium difficile. Chapters detail up-to -date experimental techniques for gene editing and transcriptional analysis which are used to investigate the fundamental biology of the organism and its virulence factors. Additional chapters describe development of potential new treatments including vaccines, bacteriophage and faecal transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Clostridium difficile: Methods and Protocols, Second Edition provides a comprehensive catalogue of molecular tools and techniques authored by the researchers who have developed them.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 304 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -This graduate-level textbook is primarily aimed at graduate students of statistics, mathematics, science, and engineering who have had an undergraduate course in statistics, an upper division course in analysis, and some acquaintance with measure theoretic probability. It provides a rigorous presentation of the core of mathematical statistics.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 404 pp. Englisch.