humana press jun 2009 (23 resultados)

Buch. Condición: Neu. Neuware - Given the popularity and utility of Saccharomyces cerevisiae, yeast-based functional genomics and proteomics technologies, developed over the past decade, have contributed greatly to our understanding of bacterial, yeast, fly, worm and human gene functions. In Yeast Functional Genomics and Proteomics: Methods and Protocols, experts in the field contribute stand-alone protocols suitable for daily use in research laboratories. The volume examines methods from the most major and fundamental techniques to more cutting-edge, advanced concepts. As part of the highly successful Methods in Molecular Biology(TM) series, the chapters are clearly formatted with introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easy to use, Yeast Functional Genomics and Proteomics: Methods and Protocols is an ideal reference for both yeast researchers and those who wish to use yeast as a model system for the further study of functional genomics and proteomics.

Buch. Condición: Neu. Neuware -Despite its inherent controversy, the exploration of the human embryo can unlock many of the answers to our deepest biological questions. In Human Embryogenesis: Methods and Protocols, internationally recognized researchers contribute detailed methods to analyze various aspects of the embryogenesis process. While comprehensively covering subjects such as the molecular mechanisms of embryonic development, in vitro fertilization, cloning, and the laws and ethical considerations of working with embryos, the volume also addresses critical features of fetal and placental development as well as of uterine biology. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and state-of-the-art, Human Embryogenesis: Methods and Protocols provides a firm foundation for the successful analysis of the embryogenesis process and an easily accessible description of the limitations and advantages of the techniques proposed, certain to aid all those who wish to further unravel the mysteries of human embryogenesis.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 324 pp. Englisch.

Buch. Condición: Neu. Neuware -in putting togetherthissetofmethods,aswellasallthemembersofmylaboratoryforproofreading and correcting the chapters and especially Sarah McClelland who read all the chapters atleasttwiceatvariousstages. Finally,IwishtothankLyndyRasmusenforherhelpin administeringtheprojectandkeepingmeorganized. Andrew D. McAinsh v Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix 1 Knock-inandKnock-out:TheUseofReverseGeneticsinSomatic CellstoDissectMitoticPathways . . . . . . . . . . . . . . . . . . . . . . . 1 AlexisR. Barr, DeborahZyssandFanniGergely 2 FunctionalDissectionofMitoticRegulatorsThroughGene TargetinginHumanSomaticCells. . . . . . . . . . . . . . . . . . . . . . . 21 EliBerdougo, Marie-EmilieTerretandPrasadV. Jallepalli 3 RNAiinDrosophilaS2CellsasaToolforStudyingCell CycleProgression. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 Monica ¿ Bettencourt-DiasandGohtaGoshima 4 ProductionofMitoticRegulatorsUsinganAutoselectionSystem forProteinExpressioninBuddingYeast. . . . . . . . . . . . . . . . . . . . 63 MarcoGeymonat, AdonisSpanosandStevenSedgwick 5 HydrodynamicAnalysisofHumanKinetochoreComplexes DuringMitosis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 SarahE. McClellandandAndrewD. McAinsh 6 IsolationofProteinComplexesInvolvedinMitosisandCytokinesis fromDrosophilaCulturedCells. . . . . . . . . . . . . . . . . . . . . . . . . 99 PierPaoloD¿Avino,VincentArchambault,MarcinR. Przewloka, WeiZhang,ErnestD. LaueandDavidM. Glover 7 AutomatedLiveMicroscopytoStudyMitoticGeneFunctionin FluorescentReporterCellLines . . . . . . . . . . . . . . . . . . . . . . . . 113 MichaelH. A. SchmitzandDanielW. Gerlich 8 ElectronTomographyofMicrotubuleEnd-Morphologies inC. elegansEmbryos. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135 EileenO¿TooleandThomasMuller-Reicher ¿ t 9 DissectingMiHumana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 360 pp. Englisch.

Buch. Condición: Neu. Neuware -One of the major challenges currently facing the scientific community is to understand the function of the multitude of protein-coding genes that were revealed when the human genome was fully sequenced. In Transgenesis Techniques: Principles and Protocols, Third Edition, experts in the field fully update and expand upon the previous edition in order to detail the transgenic techniques currently used to modify the genome. The volume explores classic procedures to genetically modify mice and other model organisms, as well as cutting-edge practices involving microinjection, site-specific recombination systems, cryopreservation, and many other topics. Written in the highly successful Methods in Molecular Biology¿ series format, the chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.Comprehensive and state-of-the-art, Transgenesis Techniques: Principles and Protocols, Third Edition is a comprehensive practical guide to the generation of transgenic animals and an invaluable source of information in any lab currently involved in transgenic techniques.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 348 pp. Englisch.

Buch. Condición: Neu. Neuware -Since the development of the concept of ¿proteomics¿, great progress has granted deeper insight into the cellular network, but the enormous range of protein abundance, dynamics, and interactions leaves many further challenges in the field. In Proteomics: Methods and Protocols, experts in proteomic research have contributed detailed techniques, which present an overview of the contemporary challenges and possibilities in the various areas of proteomics, as well as cutting-edge protocols that provide concrete examples for successful proteome analysis. Written in the clear and concise Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easy-to-use, Proteomics: Methods and Protocols will serve as a valuable reference book for laboratories around the world and raise interest in the complex and vital field of proteomics research.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 456 pp. Englisch.

Buch. Condición: Neu. Neuware -After 17 years of private practice as a cardiovascular surgeon, my partners qu- tionedtherationalityofmydecisiontoleavetheclinicalpracticebehindandbecome acardiovascular pathologist. Infact,theirdisbeliefofmyintentiontomakethe¿leap of faith¿ was understandable. For a surgeon, the operating room is where the action is. It is as simple as that. And when a cardiac surgeon can hold in his hand a beating heart, now off-bypass and improved by an operation just completed, satisfaction is real and profound. However, life is complex. Throughout my surgical career, questions regarding the pathogenesis of atherosclerotic cardiovascular disease arose; curiosities of va- ous phenotypes of the disease piqued my interest. I became aware of the power of investigative techniques that might address these questions. I then began to realize that my career in the operating room left me little time to address them. I needed to study the disease full time in order to contribute to my understanding of it. Ironically, my rst autopsy as a pathology resident was on an individual with a past history of coronary artery bypass surgery. When it came to examining the heart, the dissection, as all pathologists know, was complex. However, I found it to be straightforward and enjoyable. But I subsequently learned that my fellow re- dents and mentors did not share my intrigue and comfort in de ning the nuances of the operated heart.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 204 pp. Englisch.

Buch. Condición: Neu. Neuware -Over the last 20 years, pediatric otolaryngology has become a recognized subspecialty within otolaryngology head and neck surgery. Organizing the growth of clinical practice and knowledge in this area now is Pediatric Otolaryngology for the Clinician, a user-friendly resource for practicing general otolaryngologists, pediatricians and family practice physicians. This important title is divided into five sections: general ENT topics, otology, rhinology, head and neck disorders, and emergencies. Each chapter is authored by a recognized expert in the field and is concise and highly informative. Designed as a quick reference guide on a variety of topics such as antibiotic treatment of ear infections, sleep disorders in children, cochlear implantation, and airway management, to name just several, the book serves as a comprehensive yet succinct guide to caring for children with ear, nose and throat problems and will stand as an invaluable resource for any busy pediatric clinic.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 272 pp. Englisch.

Buch. Condición: Neu. Neuware -Nutrition and Metabolism: Underlying Mechanisms and Clinical Consequences brings together internationally recognized experts to comprehensively review our current understanding of how nutrition interacts with the genetic substrate as well as environmental-exogenous factors, including physical activity or the lack thereof, to result in insulin resistance and the metabolic syndrome.After presenting the scope of the problem, the first major part of the book is devoted to genetics and pathophysiology, the second part of the book presents the public health perspective of the most prevalent problems associated with nutrition and the metabolic syndrome, whereas the third major part of the book focuses on clinical assessment and management of the main disease states associated with inappropriate nutrition and the metabolic syndrome. Finally, general information useful for both clinicians and researchers alike is presented in the Appendix.Nutrition and Metabolism: Underlying Mechanisms and Clinical Consequences offers the reader an up-to-date and authoritative review of the major scientific and clinical aspects of the overlapping areas between nutrition and metabolism.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 452 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware - Groundbreaking and long overdue, Essential Dance Medicine is a unique text designed to help medical professionals learn the presentations, differential diagnoses and available treatment options for common dance injuries. As different types of dance have evolved, so have their related injuries. This novel text explains the underlying principles associated with correct ballet, modern and ethnic dance movements to better understand the pathophysiology and mechanism of action for the injuries described. It provides further insight by elucidating common errors and compensations dancers often make in an effort to achieve correct positioning and technique.Describing different types of dance injuries according to body region, each chapter is organized by case reports that depict a typical patient, followed by the epidemiology and pathophysiology associated with the dancer's injury. The history, physical examination findings, imaging and diagnostic evaluation for each condition are discussed. Non-operative and surgical treatment options are described according to chronicity and stage of severity of the injury. To provide evidence behind the algorithms of treatment and to highlight applicable research, relevant studies are cited as often as possible. Classic texts are also referenced to provide more in-depth information.Certain to become a gold standard in the field, Essential Dance Medicine is an important new text that provides medical professionals with the necessary tools to treat amateur and professional dancers and help them prolong their dance careers.

Buch. Condición: Neu. Neuware -In medical practice there are often complex cases that make extraordinary demands on a clinician¿s knowledge and ingenuity. In Challenging Cases in Allergy and Immunology, the authors provide these types of fascinating, and informative cases to complement a basic knowledge of diagnosis and management in these fields. As such, this novel title will be of interest to a wide array of allergists and clinical immunologists, as well as all students of medicine and allied health. Written by a multidisciplinary group of national and international experts, the book covers in detail two or more challenging, real-life cases in each disorder discussed. Each case is followed by a work-up, treatment plan, and discussion that includes a differential diagnosis, up-to-date references, and up to ten thought-provoking, multiple-choice questions that enhance the book¿s value as a comprehensive reference and teaching text. The cases include not only the common allergic diseases such as allergic rhinitis, asthma, and food allergy, but also disorders in immune regulation, autoimmunity, and immunodeficiencies. There are chapters on subjects as wide-ranging as anaphylaxis, occupational asthma, and immune-mediated rheumatic diseases. State-of-the-art and evidence-based, Challenging Cases in Allergy and Immunology is an important resource for all clinicians searching for ways to improve their ability to diagnose and manage patients with allergic and immunologic problems.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 348 pp. Englisch.

Buch. Condición: Neu. Neuware - This is the second edition of the comprehensive, concise summary of apoptosis research. It covers the major concepts, molecular architecture, the biochemical pathways, and pathophysiological significance of apoptosis. This book provides a guideline of standard biochemical and cell biologic approaches to apoptosis bench work with an emphasis on translational clinical applications for immune disorders, cancer research, ischemia, and neuronal degeneration. Since the original publication in 2003, the apoptosis field has expanded rapidly - chapters not only need to be revised and expanded, but there is a need for all new chapters covering exciting advances in bioinformatics, systems biology, oxidative stress, etc.

Buch. Condición: Neu. Neuware -Over the past three decades, carotid artery stenting has evolved to become a promising and viable alternative to carotid endarterectomy, especially for patients deemed to have high surgical risks. In Carotid Artery Stenting: The Basics, Jacqueline Saw and a panel of international experts on carotid artery stenting discuss in depth the details of all contemporary aspects of carotid stenting, while reviewing supporting studies, guidelines, technical perspectives, and peri-procedural management. This textbook serves as a learning resource on the multifaceted management of patients with carotid artery stenosis, with the key focus on extracranial carotid artery stenting. Additional sections detail the specifics of setting up and maintaining a laboratory and discuss the preparation of the carotid artery stenting procedure from both the patient and operator¿s perspective. Authoritative and highly practical, Carotid Artery Stenting: The Basics is an accessible guide and valuable resource for today¿s cardiologists, radiologists, and vascular surgeons.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 288 pp. Englisch.

Buch. Condición: Neu. Neuware - Twenty-five years ago, Earl R. Stadtman, PhD discovered that specific enzymes regulating metabolism can be inactivated by oxidation [1]. He later showed that age-related oxidative modification contributes, at least in part, to age-related loss of function of the enzymes [2, 3]. Dr. Stadtman broke the ground for a new field of study to discover how oxidative stress contributes in significant ways to age-related cellular dysfunction and protein accumulation and that oxidation in the aging brain influences Alzheimer's disease, ischemia-reperfusion injury, amyotrophic lateral sclerosis, and lifespan [4-6]. Today, his research and mentorship have positively influenced the work of hundreds of scientists in this field. We dedicate this book to Dr. Earl R. Stadtman (1912-2008), in celebration of his passion for science and his superior collaborative and mentorship skills. This book is comprised of three sections. The first describes the valuable roles reactive oxygen species (ROS) and reactive nitrogen species (RNS) play in cellular biology. The second section provides an overview of redox imbalance injury with effects on mitochondria, signaling, endoplasmic reticular function, and on aging in general. The third section takes these mechanisms to neurodegenerative disorders and provides a state-of-the-art look at the roles redox imbalances play in age-related susceptibility to disease and in the disease processes. In the first section we attempt to answer a question posed by Dr. Stadtman, ''Why have cells selected reactive oxygen species to regulate cell signaling events'' [7].

Buch. Condición: Neu. Neuware - Stem cells, characterized by the ability to both self-renew and to generate diff- entiated functional cell types, have been derived from the embryo and from va- ous sources of the postnatal animals and human. The recent advances in stem cell research have led to a better understanding of self-renewal, maintenance, and diff- entiation of both embryonic and somatic stem cells. This has significantly increased our knowledge of cellular and developmental biology in general and will certainly continue to do so for a long time to come. Moreover, given their role in maintaining and replenishing tissues, stem cells represent a potential means of restoring tissue function and thereby treating the root cause of degenerative disease. Therefore, in parallel, we need to improve our cognizance of the challenges involved in applying stem cells in clinical settings. The current chapters highlight both of these aspects: that of understanding the 'actual' and that of developing the 'possible. ' In recognition of the growing excitement and potential of stem cells as models for both the advancement of basic science and future clinical applications, I felt it timely to edit this book in which forefront investigators would provide new findings for the use of stem cells to study various lineages and tissue types and some app- cations.