humana press humana press dez 2010 (266 resultados)

Buch. Condición: Neu. Neuware -With an increasing human population and a decreasing amount of arable land, creative improvements in agriculture will be a necessity in the coming decades to maintain or improve the standard of living. In Plant Chromosome Engineering: Methods and Protocols, expert researchers present techniques for the modification of crops and other plant species in order to achieve the goal of developing the much needed novel approaches to the production of food, feed, fuel, fiber, and pharmaceuticals. This volume examines vital topics such as transformation procedures, chromosome painting, production of engineered minichromosomes, gene targeting and mutagenesis, site specific integration, gene silencing, protein expression, chromosome sorting and analysis, protocols for generating chromosomal rearrangements, enhancer trapping, and means of studying chromosomes in vivo. As a part of the highly successful Methods in Molecular Biology¿ series, the methodological chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and professional tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Plant Chromosome Engineering: Methods and Protocols highlights the spectrum of tools currently available for modifying plant genomes and chromosomes and provides the foundation for crucial future developments.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 344 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -Altogether, the biochemical, technical and economic limitations on existing proka- otic and eukaryotic expression systems and the growing clinical demand for complex therapeutic proteins have created substantial interest in developing new expression systems for the production of therapeutic proteins. To that end, plants have emerged in the past decade as a suitable alternative to the current production systems, and today their potential for production of high quality, much safer and biologically active complex recombinant pharmaceutical proteins is largely documented. The chapters in this volume, contributed by leaders in the field, sum up the state-- the-art methods for using a variety of different plants as expression hosts for phar- ceutical proteins. Several production platforms are presented, ranging from seed- and leaf-based production in stable transgenic plant lines, to plant cell bioreactors, to viral or Agrobacterium-mediated transient expr ession systems. Currently, antibodies and their derived fragments represent the largest and most important group of biote- nological products in clinical trials. This explains why the potential of most prod- tion platforms is illustrated here principally for antibodies or antibody fragments with acknowledged potential for immunotherapy in humans. In addition, a comparison of different plant expression systems is presented using aprotinin, a commercial phar- ceutical protein, as a test system. Although multiple books and monographs have been recently published on mol- ular pharming, there is a noticeable dearth of bench step-by-step protocols that can be used quickly and easily by beginners entering this new field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 384 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -Quantitative Proteomics by Mass Spectrometry, from the Methods in Molecular Biology¿ series, is a compendium of cutting-edge protocols for quantitative proteomics, and presents the most significant methods used in the field today. The focus on mass spectrometry (MS) is integral, as MS has, and will continue to be, an essential tool in proteomics for studying complex biological systems and human diseases. This volume, written and compiled by leading quantitative proteomic experts, is an indispensable resource in the search for novel biomarkers.Quantitative Proteomics by Mass Spectrometry presents several innovative MS quantitative procedures, including a variety of methods for introducing isotopic labels and quantifying post-translational modifications. Some of these methods include growing an organism in isotope-enriched media, performing trypsin proteolysis in the presence of 18O-water, reacting protein samples with isotopically labeled reagents, quantifying relative amount of proteins without the use of any isotopic labels. Attention is also given to state-of-the-art techniques for the characterization of the phosphoproteome and tandem MS for detection of inborn errors of metabolism. Specifically, the procedure for determinations of enzymatic activity could be used for large-scale screening of newborns. The protocols in this volume expand both the breadth and depth of readily available methods for quantitative proteomic researchers using MS.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 228 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. In the post-genome era, gene arrays and proteomics have allowed us to identify hitherto unknown unrecognized pathways and mechanisms. However, rather than diminish the importance of in situ hybridization, the now widespread use of screening te- nologies has increased the need to temporally and spatially localize the dist- bution of mRNA expression. Our intention, in In Situ Hybridization Protocols is to provide ample inf- mation for novices planning to set up the in situ hybridization technique and use it in their laboratory for the first time, as well as giving updates of recent developments for those laboratories where in situ hybridization techniques are already in use. Despite its widespread significance, in situ hybridization has retained a re- tation as one of the more difficult and capricious molecular biological te- niques. This may in part be because of the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in In Situ Hybridization Protocols by researchers active in the field should make it p- sible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique s- cessfully in their laboratories.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 284 pp. Englisch.

Buch. Condición: Neu. Neuware -In recent years, molecular imaging techniques have grown to be invaluable tools for molecular biology research and, to a more modest extent, clinical medicine. In Molecular Imaging: Methods and Protocols, expert researchers explore the latest advances in the field, describing a rich variety of practical procedures and methods for diverging imaging technologies. Chapters are divided into sections that cover the imaging of basic molecular and biochemical events, imaging in pre-clinical settings, and imaging in clinical settings. Composed in the highly successful Methods in Molecular Biology¿ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Comprehensive and ground-breaking, Molecular Imaging: Methods and Protocols is an essential handbook for students, established practitioners, and research fellows alike.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 264 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -Although our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned. The application and continuing development of the new, powerful biochemical and biophysical methodologies described here are essential in this quest. In The Nucleus, researchers from more than forty leading international laboratories describe state-of-the-art methods for isolating nuclei and their components and for studying their structure and activities, including some pathology-associated features. Volume I: Nuclei and Subnuclear Structures presents an overview of features of the intranuclear environment, followed by the most recent procedures for isolating nuclei from a wide range of cell types including muscle cells, yeast, oocytes, cells with polytene nuclei, Arabidopsis, trypanosomes, and dinoflagellates. The latest methods are described for isolating and working with nucleoli, constitutive heterochromatin, pathology-associated inclusions, and chromatin and for examining glycosylation, sumoylation, and ADP-ribosylation of nuclear proteins. Written in the highly successful Methods in Molecular Biology¿ series format, chapters contain lists of necessary materials and reagents, readily reproducible protocols, and tips for troubleshooting and avoiding known pitfalls. The Nucleus, Volume I: Nuclei and Subnuclear Structures is an essential reference for scientists who are working on our rapidly growing understanding of the cell nucleus and its activities.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 352 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -Recognized scientists and clinicians from around the world discuss the most recent molecular approaches to understanding the cardiovascular system in both health and disease. The authors focus on all components of the system, including blood vessels, heart, kidneys, and the brain, and cover disease states ranging from vascular and cardiac dysfunction to stroke and hypertension. The methods described for identifying the genes that cause susceptibility to cardiovascular diseases emphasize the possibility of discovering new drug targets. Authoritative and ground-breaking, Cardiovascular Genomics offers an unprecedented examination of both the cutting-edge scientific approaches now possible and the results obtained from them in the new science of cardiovascular genomics.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 376 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -Oxidative Stress in Aging: From Model Systems to Human Diseases discusses the role of free radicals in aging in different animal models, as well as the relevance of free radicals on age-related diseases and pathological conditions in humans (following an introduction section of the basics and theory of free radicals). Human aging is a complex phenomenon - not everyone gets the same diseases and dies from the same cause. Accumulating reports implicate the connection between free radicals and various diseases and age-related pathological conditions. Although the causal relationships have not been established, it is necessary to discuss how free radicals are involved in each situation. In addition, the major interventions trials of antioxidant supplements in age-related disease, cancer and so forth are reviewed and discussed.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 336 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -Sexual dysfunction presents a major challenge to physicians who take on the task of treating men with prostate cancer. While curing the disease and saving a life is paramount, improving technologies and therapies offer skilled surgeons and clinical oncologists the opportunity to eradicate the disease without compromising sexual function. In Sexual Function of the Prostate Cancer Patient, John Mulhall and a panel of internationally recognized experts on prostate cancer and sexual function assess in detail the current state of prostate cancer treatment. The authors offer a broad overview of the pathophysiology and treatment of erectile dysfunction in men with prostate cancer, reviewing the latest findings regarding erection-sparing radiation therapy and sexual function outcomes after laparascopic and robotic prostatectomy. Additional chapters discuss intra-operative maneuvers to minimize post-operative erectile dysfunction and pharmacologic penile preservation and rehabilitation. Timely and authoritative, Sexual Function in the Prostate Cancer Patient is a highly readable guide to preserving post-prostate cancer sexual function that no urologist or radiation and medical oncologist should be without.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 288 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -The era of cardiac rehabilitation in the United States dates back at least thirty years, when Herman Hellerstein at Case Western Reserve, Andy Wallace at Duke and Ken Cooper in Dallas envisioned that a comprehensive lifestyle approach to the rehabi- tation and prevention of patients having had a cardiac event would potentially yield great benefits for the individual patient and the health care system. Until that time, the thought of vigorous exercise in the cardiac patient soon after an event was close to anathema. One of us (WEK) was introduced to Herman Hellerstein in Cleveland in the late 1960¿s, when his father sought medical opinion from him for a cardiac condition. WEK was introduced to Andy Wallace in 1979 by which time the latter had started a multidisciplinary, geographically regional cardiac rehabilitation program at Duke based upon consultations with Hellerstein and Cooper. By then, cardiac rehab- itation was progressing beyond the vision of exercise only, and since then the concept of cardiac rehabilitation has grown into the comprehensive multidisciplinary program that we know today and that we attempt to describe in this volume. The practice of cardiac rehabilitation has grown and metamorphosed in the last thirty years in parallel with the growth and metamorphosis of the practice of card- vascular medicine. During the formative stages of cardiac rehabilitation, the use of coronary care units was in its infancy. The coronary artery bypass operation was less than ten years old. The LIMA bypass had not been invented.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 324 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -A comprehensive review of the use of global gene expression profiling to understand human tumors. The authors focus on the analysis of human tissue samples for a variety of cancers, including breast, colorectal, lung, renal, ovarian, bone, and brain tumors, among others. A primer on the technology and its novel analytical methods. Contains over 40 color images. Explains both the theory and practice of comprehensive gene expression profiling. Timely and topical, Expression Profiling of Human Tumors: Diagnostic and Research Applications offers every oncologist, pathologists, and cancer surgeons an essential introduction to the most promising new high-throughput investigative approaches in molecular biology-technology that is already dramatically reshaping the future of cancer research, diagnostic pathology, and clinical oncology.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 412 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -A collection of both well-established and cutting-edge methods for investigating breast cancer biology not only in the laboratory, but also in clinical settings. These readily reproducible techniques solve a variety of problems, ranging from how to collect, store, and prepare human breast tumor samples for analysis, to analyzing cells in vivo and in vitro. Additional chapters address the technology of handling biopsies, new methods for analyzing genes and gene expression, markers of clinical outcome and progress, analysis of tumor-derived proteins and antigens, validating targets, and investigating the biology of newly discovered genes.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 532 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -A multidisciplinary survey of our current understanding of the biological and clinical aspects of vascular disease. The authors describe its basic mechanisms, its clinical characteristics, its pharmacological management, and the use of neuroimaging methods to investigate it. The complex relationship between VaD and AD is also fully explored with chapters on how these processes interact and how one disease may lower the threshold for clinical expression of the other.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 372 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -Increasing attention is currently being directed to a variety of health disorders that are either unique to or more common among women than men. This volume adds sleep disorders to the list of those in which important gender differences have been neglected. Sleep Disorders in Women: A Guide to Practical Management - plores the reasons for gender bias in this area of research and provides a comp- hensive and in-depth review of what is currently known and what still remains to be studied concerning this important topic. As several contributors to this volume indicate, numerous misconceptions exist due, not surprisingly, to a shortage of reliable data in the field. As an example, obstructive sleep apnea (OSA) is typically thought to be far more common in men than in women. However, as contributors to this volume carefully document, the prevalence of OSA in women varies considerably depending on the specific female population that is studied. It is low in premenopausal women, higher in postme- pausal women, particularly high in women with polycystic ovary syndrome, and of uncertain prevalence in pregnancy. This exemplifies the fact that as women move through varying hormonal environments including puberty, the menstrual cycle, use of oral contraceptives, pregnancy, the postpartum period, and menopause, their risk for a variety of sleep disorders varies considerably. As pointed out in Dr. 288 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -The hematopoietic stem cell (HSC) field has rapidly grown in the past several years as new technologies have been developed and the older tried and true methods have been used in new ways. Major advances in the isolation of HSC and progenitor subsets have been sparked by the gene expression array technologies, leading to the identification of new markers for prospective isolation. The first edition of Hematopoietic Stem Cell Protocols is a very thorough resource covering historically the basic techniques that have been published extensively over many years. To put together, a follow-up to this excellent book has been a hard act to follow. The intent of this book is to be distinct and not duplicate but to build on the strong basic assays and include updated approaches for the combination of phenotypic and functional analyses. This second edition of Hematopoietic Stem Cell Protocols thus aims to provide timely protocols developed in the HSC field since the last comprehensive review in this series. The approaches described in this book should be readily useful for the basic science researcher, especially those familiar with the use of mouse genetic models. The chapters in this book are geared toward the laboratory-based researcher and the development of pre-clinical studies to apply HSCs toward treating blood diseases more effectively.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 328 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -A comprehensive and authoritative review of the most important scientific and clinically relevant topics today in ligaments, tendons, and capsular biology, including their biomechanics and surgical reconstruction. The authors review the basic science of tendons in the hand and shoulder ligaments, the current clinical status of the shoulder and cruciate ligaments, and the latest advances in research on the healing of ligament and tendon to bone, artificial ligaments, and gene therapy. They also cover the major type 1 collagen soft tissues that are of particular interest to upper extremity surgeons and sports medicine specialists.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 336 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields. In Electroporation Protocols: Preclinicaland Clinical Gene Medicine, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA, oligo-DNA, and RNA into targeted immune cells, tissues, and model animals in order to better treat disease. Following the successful Methods in Molecular Biology¿ series format, each method and protocol offers step-by-step, readily reproducible laboratory instructions, with the benefit of several introductory reviews to aid investigators new to this exciting technology.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 548 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -Although there has been an explosion of interest and technology in the study of neural stem cells, many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. Neural Stem Cells, 2nd Edition revises and expands upon the successful first edition in order to provide the most current, cutting-edge methods of today for the scientists working to answer these questions. The use of these step-by-step, readily reproducible laboratory protocols will allow investigators to produce pure populations that can serve as a means of understanding the biology of neural stem cells and adapting them for transplantation into disease models. In addition to the topics covered in the first edition, this new volume provides recently developed alternative methods for both human and rodent NSCs, along with chapters on karyotyping, migration, apoptosis, and NSC markers. Following the Methods in Molecular Biology¿ series format, each chapter has an extended introduction for background, a list of necessary materials, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and current, Neural Stem Cells, 2nd Edition is an excellent source of information and inspiration for scientists longing to use these powerful cells against neurogenetic and neurodegenerative diseases.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 424 pp. Englisch.

Buch. Condición: Neu. Neuware -Of all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques, all of which have been producing fundamental insights into the functions of cells and tissues. Chapters cover a variety of bright field and fluorescence microscopy-based approaches that are central to the study of a range of biological questions, providing information on how to prepare cells and tissues for microscopic investigations, covering detailed staining procedures, and exploring methods to analyze images and interpret the results accurately. Composed in the highly successful Methods in Molecular Biology¿ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Comprehensive and current, Light Microscopy: Methods and Protocols is an essential handbook for all researchers who are exploring the intriguing microscopic world of the cell.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 256 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -Focusing on two-dimensional electrophoresis (2-DE), this book sets forth the principles and recent developments of sample preparation and fractionation tools in expression proteomics. Step-by-step instructions alongside detailed illustrations enable you to quickly learn how to perform the methods yourself. Tricks of the trade and notes on troubleshooting and avoiding pitfalls help you ensure successful results every time. The methods covered in this book are not limited to 2-DE; they can also be applied to a broad range of protein separation techniques.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 356 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -Over the past few years, DNA methylation technologies and our knowledge of DNA methylation patterns have been advancing at a breathtaking pace. Due to this fact, DNA Methylation: Methods and Protocols, Second Edition completely revises, updates, and expands upon the popular first edition with the most current novel techniques, easier to use and more refined by the tested experience of leading experts. This revision reflects contemporary study of the subject: the analysis of gene-specific DNA methylation patterns has been complemented by genome-wide approaches, and epigenomics takes a central place. Written in the highly successful Methods in Molecular Biology¿ series format, the chapters in this volume present brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, DNA Methylation: Methods and Protocols, Second Edition is the perfect resource for scientists who wish to further our understanding of the molecular processes that determine the genomic DNA methylation landscape and for those who aim to identify and implement DNA methylation-based biomarkers for clinical and diagnostic applications.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 420 pp. Englisch.

Buch. Condición: Neu. Neuware -Chronic obstructive pulmonary disease (COPD) affects millions of people across the world. COPD is not only a major burden to patients but is also costly and results in billions of dollars of direct and indirect costs annually. In recent years and with advancement of science, the understanding of COPD has improved significantly. Fortunately, current management guidelines consider COPD a preventable and treatable condition, and recent studies clearly indicate that available pharmacological and non-pharmacological interventions may improve various clinical outcomes. COPD: A Guide to Diagnosis and Clinical Management offers an exciting, evidence-based assessment of the field and will be of significant interest to clinicians who care for patients with COPD, including primary care providers and specialists. Comprehensive and state-of-the-art, this title is authored by experts who took the task of developing a resource that focuses on the essential issues in caring for patients with COPD. The first four chapters of the book cover major points about the systemic nature of COPD, the clinical and physiological assessments, and the outcome measures and prognostic markers. In the following section, various pharmacologic and non-pharmacological management strategies are reviewed based on the available evidence. The final sections outline the non-pulmonary effects of COPD and their management. COPD: A Guide to Diagnosis and Clinical Management is a vital, evidence-based text that will prove invaluable for all clinicians who care for patients with this debilitating disorder.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 336 pp. Englisch.

Buch. Condición: Neu. Neuware -Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology¿ format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 324 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -Due to their vital involvement in a wide variety of housekeeping and specialized cellular functions, exocytosis and endocytosis remain among the most popular subjects in biology and biomedical sciences. Tremendous progress in understanding these complex intracellular processes has been achieved by employing a wide array of research tools ranging from classical biochemical methods to modern imaging techniques. In Exocytosis and Endocytosis, skilled experts provide the most up-to-date,step-by-step laboratory protocols for examining molecular machinery and biological functions of exocytosis and endocytosis in vitro and in vivo. Following the highly successful Methods in Molecular Biology¿ series format, the chapters present an introduction outlining the principle behind each technique, a list of the necessary materials, an easy to follow, readily reproducible protocol, and a Notes section offering tips on troubleshooting and avoiding known pitfalls.Insightful to both newcomers and seasoned professionals, Exocytosis and Endocytosis offers a unique and highly practical guide to versatile laboratory tools developed to study various aspects of intracellular vesicle trafficking in simple model systems and living organisms.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 428 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 412 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric- tural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger ¿gene silencing¿ often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Ag- bacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent devel- ments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 512 pp. Englisch.

Buch. Condición: Neu. Neuware -Despite wide recognition as a serious public health problem, anaphylaxis and hypersensitivity reactions remain under-recognized and under-diagnosed. This book fills the gaps in our understanding of the identification of triggers, recognition of clinical presentations, understanding of the natural history of these reactions, and selection of treatment strategies including those focused on cellular and molecular targets. The book provides a detailed examination of disease etiology, pathogenesis, and pathophysiology and their correlation to clinical practice. Forefront knowledge of the mediators and mechanisms of anaphylaxis is covered with an emphasis on how new discoveries shape our current and emerging therapies.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 376 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -A discussion of all the key issues in the use of human pluripotent stem cells for treating degenerative diseases or for replacing tissues lost from trauma. On the practical side, the topics range from the problems of deriving human embryonic stem cells and driving their differentiation along specific lineages, regulating their development into mature cells, and bringing stem cell therapy to clinical trials. Regulatory issues are addressed in discussions of the ethical debate surrounding the derivation of human embryonic stem cells and the current policies governing their use in the United States and abroad, including the rules and conditions regulating federal funding and questions of intellectual property.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 480 pp. Englisch.

Buch. Condición: Neu. Neuware -Scientists investigating germ cells have, over the past 15 years, originated discoveries and innovations that give us valuable insights into the mechanisms that regulate not just stem cell function, but human development in its widest sense. With contributions from some of the leading researchers in the field, Male Germline Stem Cells: Developmental and Regenerative Potential assesses the implications of these discoveries for understanding the fundamental biology of germline stem cells as well as their potential for human stem cell-based therapies. This monograph covers many of the fundamental issues now being explored by today¿s generation of stem cell researchers, including the field¿s potential for regenerative medicine. Ranging from an assessment of the pluripotency of primordial germ cells and their possible applications in treating testicular cancer, to the recovery of once-mordant fertilization-competent sperm, this volume has it all. It is a reference point for any scientist involved in related research as well as being a timely summation of what could prove to be a hugely exciting and very fruitful area of inquiry.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 280 pp. Englisch.

Taschenbuch. Condición: Neu. Neuware -A powerful collection of readily reproducible cutting-edge techniques for characterizing the ligand or substrate binding of neurotransmitter receptors and transporters. The procedures cover interdisciplinary interactions for monoamine transporters, amino acid transporters, ionotropic receptors, metabotropic glutamate receptors, GABA receptors, and other G protein-coupled receptors. By illuminating how neurons in the central nervous communicate with other, these techniques can lead to the development of novel therapeutic strategies for neurological diseases.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 292 pp. Englisch.