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Añadir al carritoCondición: Sehr gut. Zustand: Sehr gut | Seiten: 416 | Sprache: Englisch | Produktart: Bücher | The aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twenty-five years ago when the first papers were published, it has since experienced exponential growth, and in most countries around the world there are laboratories working on plant proteomics. Two-dimensional gel electrophoresis is still the basic method used, but it has been improved greatly with IPG in the first dimension (Chapter 13) and with new detection methods with fluorochromes (Chapters 14 and 15). Signi- cant progress has been achieved in protein extraction, which is particularly difficult with plant tissues containing phenols, proteases, and other secondary metabolites that interfere with proteins. Standard procedures have been op- mized (Chapters 1 and 2) for peculiar tissues (Chapters 3, 4, and 5) and cellular compartments (Chapters 6 to 10). These methods rely on improvements made in the solubilization of proteins from membranes (Chapters 11 and 12). Mass spectrometry was a revolution that permitted the high throughput identifi- tion of proteins separated by 2D gels (Chapters 19 and 20) but also from blue native 1D gels (Chapters 27 and 28) despite the fact that Edman sequencing can still be useful (Chapter 18). Associated with other techniques such as 2DLC or LC of intact proteins, mass spectrometry also permits the identification of polypeptides from complexes (Chapters 21 and 22).
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Añadir al carritoCondición: New. Editor(s): Thiellement, Herve. Series: Methods in Molecular Biology. Num Pages: 416 pages, 135 black & white illustrations, biography. BIC Classification: PST. Category: (P) Professional & Vocational. Dimension: 229 x 152 x 23. Weight in Grams: 608. . 2010. 1st ed. Softcover of orig. ed. 2007. Paperback. . . . .
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Añadir al carritoCondición: New. Aims to provide methods and protocols used by recognized scientists in the world of plant proteomics. This work explains their experimental methodology, either in the wet or in the dry lab, enabling a novice to undertake the described method. Editor(s): Thiellement, Herve. Series: Methods in Molecular Biology. Num Pages: 416 pages, 135 black & white illustrations, biography. BIC Classification: PSD; PST. Category: (P) Professional & Vocational. Dimension: 229 x 152 x 26. Weight in Grams: 781. . 2006. Hardback. . . . .
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Añadir al carritoTaschenbuch. Condición: Neu. Plant Proteomics | Methods and Protocols | Hervé Thiellement | Taschenbuch | 416 S. | Englisch | 2010 | Humana Press | EAN 9781617376863 | Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu.
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Añadir al carritoCondición: New. Includes supplementary material: sn.pub/extrasThe aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twen.
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Añadir al carritoCondición: New. Editor(s): Thiellement, Herve. Series: Methods in Molecular Biology. Num Pages: 416 pages, 135 black & white illustrations, biography. BIC Classification: PST. Category: (P) Professional & Vocational. Dimension: 229 x 152 x 23. Weight in Grams: 608. . 2010. 1st ed. Softcover of orig. ed. 2007. Paperback. . . . . Books ship from the US and Ireland.
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Añadir al carritoCondición: New. Aims to provide methods and protocols used by recognized scientists in the world of plant proteomics. This work explains their experimental methodology, either in the wet or in the dry lab, enabling a novice to undertake the described method. Editor(s): Thiellement, Herve. Series: Methods in Molecular Biology. Num Pages: 416 pages, 135 black & white illustrations, biography. BIC Classification: PSD; PST. Category: (P) Professional & Vocational. Dimension: 229 x 152 x 26. Weight in Grams: 781. . 2006. Hardback. . . . . Books ship from the US and Ireland.
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Añadir al carritoBuch. Condición: Neu. Neuware - The aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twenty-five years ago when the first papers were published, it has since experienced exponential growth, and in most countries around the world there are laboratories working on plant proteomics. Two-dimensional gel electrophoresis is still the basic method used, but it has been improved greatly with IPG in the first dimension (Chapter 13) and with new detection methods with fluorochromes (Chapters 14 and 15). Signi- cant progress has been achieved in protein extraction, which is particularly difficult with plant tissues containing phenols, proteases, and other secondary metabolites that interfere with proteins. Standard procedures have been op- mized (Chapters 1 and 2) for peculiar tissues (Chapters 3, 4, and 5) and cellular compartments (Chapters 6 to 10). These methods rely on improvements made in the solubilization of proteins from membranes (Chapters 11 and 12). Mass spectrometry was a revolution that permitted the high throughput identifi- tion of proteins separated by 2D gels (Chapters 19 and 20) but also from blue native 1D gels (Chapters 27 and 28) despite the fact that Edman sequencing can still be useful (Chapter 18). Associated with other techniques such as 2DLC or LC of intact proteins, mass spectrometry also permits the identification of polypeptides from complexes (Chapters 21 and 22).
Idioma: Inglés
Publicado por SPRINGER NATURE Nov 2010, 2010
ISBN 10: 1617376868 ISBN 13: 9781617376863
Librería: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Alemania
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Añadir al carritoTaschenbuch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twenty-five years ago when the first papers were published, it has since experienced exponential growth, and in most countries around the world there are laboratories working on plant proteomics. Two-dimensional gel electrophoresis is still the basic method used, but it has been improved greatly with IPG in the first dimension (Chapter 13) and with new detection methods with fluorochromes (Chapters 14 and 15). Signi- cant progress has been achieved in protein extraction, which is particularly difficult with plant tissues containing phenols, proteases, and other secondary metabolites that interfere with proteins. Standard procedures have been op- mized (Chapters 1 and 2) for peculiar tissues (Chapters 3, 4, and 5) and cellular compartments (Chapters 6 to 10). These methods rely on improvements made in the solubilization of proteins from membranes (Chapters 11 and 12). Mass spectrometry was a revolution that permitted the high throughput identifi- tion of proteins separated by 2D gels (Chapters 19 and 20) but also from blue native 1D gels (Chapters 27 and 28) despite the fact that Edman sequencing can still be useful (Chapter 18). Associated with other techniques such as 2DLC or LC of intact proteins, mass spectrometry also permits the identification of polypeptides from complexes (Chapters 21 and 22). 416 pp. Englisch.