Laboratory experts present cutting-edge molecular biology techniques for studying the mycobacteria, a medically significant group of organisms responsible for both human and animal disease, including tuberculosis and leprosy. The methods range from basic culture techniques, cell fractionation, and nucleic acid purification to more advanced protocols for the introduction of cloned DNA into mycobacteria, the use of reporter genes, and the expression of foreign genes. There are also important techniques for mycobacteriophages, genome analysis, chemical and transposon mutagenesis, gene replacement, and analysis of gene expression, as well as such clinically useful techniques as antibiotic susceptibility testing, speciation, and RFLP analysis.

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this book is an essential purchase for an laboratory dealing with mycobacteria and good value for individual researchers. - Microbiology Today a unique book presents excellent description of many of the molecular biological tools that have been developed an invaluable collection of methodologies for mycobacterial research the molecular mycobacteriologist will find much of interest in this book Worth getting. - Journal of Medical Microbiology ...this book provides a thorough guide to many of the techniques currently used to study the Mycobacteria. Comprehensive treatment of the subject includes both molecular techniques and the basic microbiological methods required for manipulation of these organisms...of interest to the epidemiologist is the description of computer analysis of DNA fingerprinting for tuberculosis while the chapter on accessing MycDB, a database dedicated to mycobacterial research, will be of value to all those working in this area. - Molecular Biotechnology this book provides a thorough guide to many of the techniques currently used to study the Mycobacteria. Comprehensive treatment of the subject includes both molecular techniques and the basic microbiological methods required for manipulation of these organisms. The detailed technical nature of each chapter provides clear instruction for those wishing to engage in this area of research. - Molecular Biotechnology

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