Mouse Models of Innate Immunity: Methods and Protocols (Methods in Molecular Biology, 1031)

1. Conventional Murine Gene Targeting

Albert G. Zimmermann and Yue Sun

2. Production and Characterization of Humanized Rag2^-/-γc^-/- Mice

Freddy M. Sanchez, German I. Cuadra, Stanton J. Nielsen, Anne Tanner, and Bradford K. Berges

3. Isolation, Culture, and Functional Evaluation of Bone Marrow-Derived Macrophages

Beckley K. Davis

4. Collecting Resident or Thioglycollate Elicited Peritoneal Macrophages

Monika Schneider

5. Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils

Linda Vong, Philip M Sherman, and Michael Glogauer

6. Assessment of Oxidative Metabolism

Emilie Imbeault and Denis Gris

7. Generation and Culture of Mouse Embryonic Fibroblasts

Yu Lei

8. Primary Ear Fibroblast Derivation from Mice

Chris B. Moore and Irving C. Allen

9. Bone Marrow Derived Dendritic Cells

Kelly Roney

10. Measuring T Cell Function in Innate Immune Models

Brianne R. Barker

11. Bioassay for the Measurement of Type-I Interferon Activity

Douglas G. Widman

12. Safe and Effective Mouse Foot-Pad Inoculation

Kristin M. Long and Mark Heise

13. Delayed Type Hypersensitivity Models in Mice

Irving C. Allen

14. Mouse Model of Staphylococcus aureus Skin Infection

Natalia Malachowa, Scott D. Kobayashi, Kevin R. Braughton, and Frank R. DeLeo

15. Sepsis Induced by Cecal Ligation and Puncture

Haitao Wen

16. Systemic Infection of Mice with Listeria monocytogenes to Characterize Host Immune Responses

Nancy Wang, Richard A. Strugnell, Odilia L. Wijburg, and Thomas C. Brodnicki

17. Mouse Model of Invasive Fungal Infection

Donna M. MacCallum

18. Endotoxin-Induced Uveitis in Rodents

Umesh C.S. Yadav and Kota V. Ramana

19. Bacteria-Mediated Acute Lung Inflammation

Irving C. Allen

20. Intranasal Influenza Infection of Mice and Methods to Evaluate Progression and Outcome

Catherine J. Sanders, Brian Johnson, Charles W. Frevert, and Paul G. Thomas

21. Dextran Sodium Sulfate-Induced Murine Inflammatory Colitis Model

Monika Schneider

22. Bacterial-Mediated Gastrointestinal Inflammation

Joshua Uronis and Xiaolun Sun

23. Plasmodium berghei ANKA (PbA) Infection of C57BL/6J Mice: A Model of Severe Malaria

Marcela Montes de Oca and Ashraful Haque

24. Characterization of HIV-1 Infection in the Humanized Rag2^-/-γc^-/- Mouse Model

Freddy M. Sanchez and Bradford K. Berges

The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense; however, human studies of its function are often limited by ethical, logistical, and technical obstacles. In Mouse Models of Innate Immunity: Methods and Protocols, experts in the field explore the design and execution of experiments used to thoroughly evaluate critical elements associated with the host innate immune response. The volume opens with methods that are essential for collecting and assessing various primary cells that are highly relevant to innate immunity, and it continues with in vivo protocols commonly used to evaluate the innate immune response in the mouse, including mouse models of respiratory infection, gastrointestinal inflammation, fungal and parasitic diseases, sepsis, and HIV-1 infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune response.