Microbial Carotenoids From Fungi: Methods and Protocols (Methods in Molecular Biology, 898, Band 898)

1. Essential Role of Genetics in the Advancement of Biotechnology

Arnold L. Demain and José L. Adrio

2. Microbial Carotenoids

Preejith Vachali, Prakash Bhosale, and Paul S. Bernstein

3. Biosynthesis, Extraction, Purification and Analysis of Trisporoid Sexual Communication Compounds from Mated Cultures of Blakeslea trispora

C. Schimek and J. Wöstemeyer

4. Isolation of Mutants and Construction of Intersexual Heterokaryons of Blakeslea trispora

Enrique Cerdá-Olmedo and Bina J. Mehta

5. Molecular Tools for Carotenogenesis Analysis in the Zygomycete Mucor circinelloides

Santiago Torres-Martínez, Rosa M. Ruiz-Vázquez, Victoriano Garre, Sergio López-García, Eusebio Navarro and Ana Vila

6. Gene Fusions for the Directed Modification of the Carotenoid Biosynthesis Pathway in Mucor circinelloides

Enrique A. Iturriaga, Tamás Papp, María Isabel Alvarez, and Arturo P. Eslava

7. Integration of a Bacterial Beta-carotene Ketolase Gene into the Mucor circinelloides Genome by the Agrobacterium tumefaciens-mediated Transformation (ATMT) Method

Tamás Papp, Árpád Csernetics, Ildikó Nyilasi, Csaba Vágvölgyi and Enrique A. Iturriaga

8. Metabolic Engineering of Mucor circinelloides for Zeaxanthin Production

Marta Rodríguez-Sáiz, Juan Luis de la Fuent, José Luis Barredo

9. Bioengineering of Oleaginous Yeast Yarrowia Lipolytica for Lycopene Production

Rick W. Ye, Pamela L. Sharpe, and Quinn Zhu

10. Peroxisome Targeting of Lycopene Pathway Enzymes in Pichia pastoris

Pyung Cheon Lee

11. Production, Extraction and Quantification of Astaxanthin by Xanthophyllomyces dendrorhous or Haematococcus pluvialis: Standardized Techniques

Domínguez-Bocanegra Alma R.

12. Isolation and Selection of New Astaxanthin Producing Strains of Xanthophyllomyces dendrorhous

Diego Libkind, Martín Moliné, and Celia Tognetti

13. Isolation and Characterization of Extrachromosomal double-stranded RNA Elements in Xanthophyllomyces dendrorhous

Marcelo Baeza, María Fernández-Lobato, and Víctor Cifuentes

14. Isolation of Carotenoid Hyperproducing Mutants of Xanthophyllomyces dendrorhous (Phaffia rhodozyma) by Flow Cytometry and Cell Sorting

Byron F. Brehm-Stecher and Eric A. Johnson

15. Generation of Astaxanthin Mutants in Xanthophyllomyces dendrorhous using a Double Recombination Method Based on Hygromycin Resistance

Mauricio Niklitschek, Marcelo Baeza, María Fernández-Lobato and Víctor Cifuentes

16. Genetic Manipulation of Xanthophyllomyces dendrorhous and Phaffia rhodozyma Guangyun Lin, Joanna Bultman, Eric A. Johnson, and Jack W. Fell

17. DNA Assembler Method for Construction of Zeaxanthin-producing

Strains of Saccharomyces cerevisiae

Zengyi Shao, Yunzi Luo, and Huimin Zhao

18. Neurosporaxanthin Production by Neurospora and Fusarium

Javier Avalos, Alfonso Prado-Cabrero, and Alejandro F. Estrada

19. Production of Torularhodin, Torulene and Beta-Carotene by Rhodotorula Yeasts

Martín Moliné, Diego Libkind, and Maria van Broock

Carotenoids are a family of yellow to orange-red terpenoid pigments synthesized by photosynthetic organisms and many bacteria and fungi. They have beneficial health effects protecting against oxidative damage, and may be responsible for the colours associated with plants and animals. In Microbial Carotenoids From Fungi: Methods and Protocols, expert researchers in the field detail many of the most up-to-date methods which are now commonly used to study carotenoids. These include methods for the manipulation and metabolic engineering of the Beta-carotene producing fungi Blakeslea trispora and Mucor circinelloides, lycopene production with the yeast Yarrowia lipolytica, peroxisome targeting of lycopene pathway enzymes in Pichia pastoris, and the manipulation of the heterobasidiomycetous yeast Xanthophyllomyces dendrorhous, which produces astaxanthin, a red xanthophyll with large importance in the aquaculture, pharmaceutical, and food industries. Additionally, the book includes a DNA assembler method for construction of zeaxanthin-producing strains of Saccharomyces cerevisiae, production of neurosporaxanthin by Neurospora and Fusarium, and production of torularhodin, torulene and b-carotene by Rhodotorula yeasts. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Microbial Carotenoids From Fungi: Methods and Protocols, provides practical experimental laboratory procedures for a wide range of carotenoids producing microorganisms.