XVII, 190 S. : 62 Ill., graph. Darst. Gebraucht; Einband minimal berieben, ansonten guter Zustand. Aus dem Inhalt: An Introduction to Electron Microscops (EM) / Methods for TEM / Methods for SEM / Evaluation of Micrographs / Appendix: Buffers in Electron Microscopy / Subject Index. Sprache: Englisch Gewicht in Gramm: 350.

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T�tulo

Methods of preparation for electron microscopy : an introd. for the biomed. sciences. With a foreword by K. M�hlethaler.

Editor

Berlin ; Heidelberg ; New York ; London ; Paris ; Tokyo : Springer

Fecha de publicaci�n

1987

Idioma

Ingl�s

ISBN 10

354017592X

ISBN 13

9783540175926

Encuadernaci�n

Encuadernaci�n de tapa blanda

Estado

Gut

Peso

350 gramos

Cat�logos de vendedores

Medizin

Acerca de

Sinopsis

In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes� sions had an unexpected instrument at their disposal which exceeded the reso� lution of the light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob� lems inherent in specimen preparation for the investigation of cellular struc� tures. The microtechniques applied in light microscopy were no longer appli� cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada� tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses� sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolution, yet a suitable preparation methodology was lacking. This sceptical attitude towards the application of electron microscopy in bi� ology and medicine was supported simultaneously by the general opinion of colloid chemists, who postulated that in the submicroscopic region of living structures no stable building blocks existed which could be revealed with this apparatus.

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Kepler-Buchversand Huong Bach
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