1. Genetic Association Studies in Host-Pathogens Interaction Analysis
Jose Luis Royo and Luis Miguel Real
2. Bacterial Genotyping Methods: From the Basics to Modern
Margarita M. Ochoa-Díaz, Silvana Daza-Giovannetty, and Doris Gómez-Camargo
Part I: Animal-Pathogen Interaction Section
3. Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens
Gili Aviv and Ohad Gal-Mor
4. The Usage of a Bioluminescence Reporter System to Image Promoter Activity During Host Infection
Gili Aviv and Ohad Gal-Mor
5. lacZ Reporter System as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens
Gili Aviv and Ohad Gal-Mor
6. Western Blotting Against Tagged Virulence Determinants to Study Bacterial Pathogenicity
Gili Aviv and Ohad Gal-Mor
7. Molecular Methods to Analyze the Effect of Proteins Expressed by Salmonella During Its Intracellular Stage
Carlos Medina, Beatriz Mesa-Pereira, Eva Mª Camacho, Amando Flores, and Eduardo Santero
8. Organoids as a Model to Study Infectious Disease
Kristen A. Engevik, Andrea L. Matthis, Marshall H. Montrose, and Eitaro Aihara
9. Surface Proteome Biotinylation Combined with Bioinformatic Tools as a Strategy for Predicting Pathogen Interacting Proteins
Anita Horvatić, Josipa Kuleš, Nicolas Guillemin, Franjo Martinković, Iva Štimac, Vladimir Mrljak, and Mangesh Bhide
10. Systems Biology Modeling to Study Pathogen-Host Interactions
Müberra Fatma Cesur and Saliha Durmuş
11. Phage Therapy: Various Perspectives on How to Improve the Art
Stephen T. Abedon
Part II: Microbe-Microbe Interaction Section
12. Application of RNA-seq and Bioimaging Methods to Study Microbe-Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression
Cristina Isabel Amador, Claus Sternberg, and Lars Jelsbak
13. Serial Dilution-Based Growth Curves and Growth Curve Synchronization for High-Resolution Time Series of Bacterial Biofilm Growth
Fernando Govantes
14. Detection of Bacterial Quorum Sensing Molecules
Elke Stein and Adam Schikora
Part III: Plant-Pathogen Interaction Section
15. Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae
José S. Rufián, Diego López-Márquez, Nieves López-Pagán, Murray Grant, Javier Ruiz-Albert, and Carmen R. Beuzón
16. Introduction of Genetic Material in Ralstonia solanacearum through Natural Transformation and Conjugation
Anthony Perrier, Patrick Barberis, and Stephane Genin
17. In Vitro and In Vivo Secretion/Translocation Assays to Identify Novel Ralstonia solanacearum Type 3 Effectors
Fabien Lonjon, Nemo Peeters, Stéphane Genin, and Fabienne Vailleau
18. Plant Pathogenicity Phenotyping of Ralstonia solanacearum Strains
Alice Morel, Nemo Peeters, Fabienne Vailleau, Patrick Barberis, Gaofei Jiang, Richard Berthomé, and Alice Guidot
19. Methods to Quantify Biotic-Induced Stress in Plants
Marcel Bach-Pages and Ga
This detailed volume explores numerous multidisciplinary approaches employed to analyze the role of different molecules or strategies used by different guests to survive inside hosts. Divided into several sections, the book delves into animal-pathogen interaction, microbe-microbe interaction, as well as plant-pathogen interaction. Written for the highly successful
Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Host-Pathogen Interactions: Methods and Protocols provides many molecular and bioinformatics techniques currently in use at reputed laboratories in countries all around the world, vital for many kinds of host-pathogen interactions studies.
