1. Decellularization Methods for Scaffold Fabrication
Sweta K. Gupta and Narayan C. Mishra
2. Decellularized Liver Scaffold for Liver Regeneration
Wei Yang, Renpei Xia, Yujun Zhang, Hongyu Zhang, and Lianhua Bai
3. Wharton's Jelly Matrix Decellularization for Tissue Engineering Applications
Gabriel L. Converse, Dandan Li, Eric E. Buse, Richard A. Hopkins, and Omar S. Aljitawi
4. 3D-Decellularized Native Extra Cellular Matrix Scaffold for In Vitro Culture Expansion of Human Wharton Jelly Mesenchymal Stem Cells (hWJ MSCs)
Balasubramanian Sundaram, Anne George Cherian, and Sanjay Kumar
5. Decellularized Iliotibial Band Recolonized with Allogenic Homotopic Fibroblasts or Bone Marrow-Derived Mesenchymal Stromal Cells
Clemens Gögele, Silke Schwarz, Benjamin Ondruschka, Niels Hammer, and Gundula Schulze-Tanzil
6. Preparation of Cell-Derived Decellularized Matrices Mimicking Native ECM During the Osteogenesis and Adipogenesis of Mesenchymal Stem Cells
Takashi Hoshiba, Naoki Kawazoe, and Guoping Chen
7. Mouse Skeletal Muscle Decellularization
Martina Piccoli, Caterina Trevisan, Edoardo Maghin, Chiara Franzin, and Michela Pozzobon
8. Extracellular Matrix from Whole Porcine Heart Decellularization for Cardiac Tissue Engineering
Matthew J. Hodgson, Christopher C. Knutson, Nima Momtahan, and Alonzo D. Cook
9. In-Lab Manufacturing of Decellularized Rat Renal Scaffold for Kidney Bioengineering
Andrea Peloso, Antonio Citro, Valeria Corradetti, Szandra Branbilla, Graziano Oldani, Francesco Calabrese, Tommaso Dominioni, Marcello Maestri, and Lorenzo Cobianchi
10. Glutaraldehyde Crosslinking Modification of Decellularized Rat Kidney Scaffolds
Yaling Yu, Li Liu, Jianse Zhang, Zairong Wei, and Jin Mei
11. Preparation of Scaffolds from Decellularized Testicular Matrix
Yoni Baert and Ellen Goossens
12. Decellularization of Bovine Small Intestinal Submucosa
Mahmut Parmaksiz, A. Eser Elçin, and Y. Murat Elçin
13. Decellularization and Recellularization of Cartilage
Catherine A. Bautista and Bahar Bilgen
14. A Protocol to Prepare Decellularized Stem Cell Matrix for Rejuvenation of Cell Expansion and Cartilage Regeneration
Jingting Li and Ming Pei
15. Tracheal Cartilage Isolation and Decellularization
Zachary Galliger and Angela Panoskaltsis-Mortari
16. Protocols for Rat Uterus Isolation and Decellularization: Applications for Uterus Tissue Engineering and 3D Cell Culturing
Arvind Manikantan Padma, Tom Tristan Tiemann, Ahmed Baker Alshaikh, Randa Akouri, Min Jong Song, and Mats Hellström
17. Application of Decellularized Tissue Scaffolds in Ovarian Tissue Transplantation
Enes Taylan and Kutluk Oktay
18. Composite Bioscaffolds Incorporating Decellularized ECM as a Cell-Instructive Component within Hydrogels as In Vitro Models and Cell Delivery Systems
Arthi Shridhar, Elizabeth Gillies, Brian G. Amsden, and Lauren E. Flynn
19. 3D-Printing Composite Polycaprolactone-Decellularized Bone Matrix Scaffolds for Bone Tissue Engineering Applications
Alexandra N. Rindone, Ethan Nyberg, and Warren L. Grayson
20. Decellularization of Large Tendon Specimens: Combination of Manually Performed Freez
This practical, hands-on volume examines the use of decellularized tissues and organs as biologically-active scaffolds by providing numerous approaches from experts in the field. While knowledge of how to grow and differentiate cells in culture has dramatically improved over time, the book addresses the challenges of how to instruct particular cells of interest to recognize and respond to their environment so as to proliferate, differentiate, and function for restoration of original tissue and organ form and function. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easy to use, Decellularized Scaffolds and Organogenesis: Methods and Protocols share novel approaches and insights that will provide new opportunities for those already in the field or moving to enter the field.