Decellularized Scaffolds and Organogenesis: Methods and Protocols (Methods in Molecular Biology, 1577)

1. Decellularization Methods for Scaffold Fabrication

Sweta K. Gupta and Narayan C. Mishra

2. Decellularized Liver Scaffold for Liver Regeneration

Wei Yang, Renpei Xia, Yujun Zhang, Hongyu Zhang, and Lianhua Bai

3. Wharton's Jelly Matrix Decellularization for Tissue Engineering Applications

Gabriel L. Converse, Dandan Li, Eric E. Buse, Richard A. Hopkins, and Omar S. Aljitawi

4. 3D-Decellularized Native Extra Cellular Matrix Scaffold for In Vitro Culture Expansion of Human Wharton Jelly Mesenchymal Stem Cells (hWJ MSCs)

Balasubramanian Sundaram, Anne George Cherian, and Sanjay Kumar

5. Decellularized Iliotibial Band Recolonized with Allogenic Homotopic Fibroblasts or Bone Marrow-Derived Mesenchymal Stromal Cells

Clemens Gögele, Silke Schwarz, Benjamin Ondruschka, Niels Hammer, and Gundula Schulze-Tanzil

6. Preparation of Cell-Derived Decellularized Matrices Mimicking Native ECM During the Osteogenesis and Adipogenesis of Mesenchymal Stem Cells

Takashi Hoshiba, Naoki Kawazoe, and Guoping Chen

7. Mouse Skeletal Muscle Decellularization

Martina Piccoli, Caterina Trevisan, Edoardo Maghin, Chiara Franzin, and Michela Pozzobon

8. Extracellular Matrix from Whole Porcine Heart Decellularization for Cardiac Tissue Engineering

Matthew J. Hodgson, Christopher C. Knutson, Nima Momtahan, and Alonzo D. Cook

9. In-Lab Manufacturing of Decellularized Rat Renal Scaffold for Kidney Bioengineering

Andrea Peloso, Antonio Citro, Valeria Corradetti, Szandra Branbilla, Graziano Oldani, Francesco Calabrese, Tommaso Dominioni, Marcello Maestri, and Lorenzo Cobianchi

10. Glutaraldehyde Crosslinking Modification of Decellularized Rat Kidney Scaffolds

Yaling Yu, Li Liu, Jianse Zhang, Zairong Wei, and Jin Mei

11. Preparation of Scaffolds from Decellularized Testicular Matrix

Yoni Baert and Ellen Goossens

12. Decellularization of Bovine Small Intestinal Submucosa

Mahmut Parmaksiz, A. Eser Elçin, and Y. Murat Elçin

13. Decellularization and Recellularization of Cartilage

Catherine A. Bautista and Bahar Bilgen

14. A Protocol to Prepare Decellularized Stem Cell Matrix for Rejuvenation of Cell Expansion and Cartilage Regeneration

Jingting Li and Ming Pei

15. Tracheal Cartilage Isolation and Decellularization

Zachary Galliger and Angela Panoskaltsis-Mortari

16. Protocols for Rat Uterus Isolation and Decellularization: Applications for Uterus Tissue Engineering and 3D Cell Culturing

Arvind Manikantan Padma, Tom Tristan Tiemann, Ahmed Baker Alshaikh, Randa Akouri, Min Jong Song, and Mats Hellström

17. Application of Decellularized Tissue Scaffolds in Ovarian Tissue Transplantation

Enes Taylan and Kutluk Oktay

18. Composite Bioscaffolds Incorporating Decellularized ECM as a Cell-Instructive Component within Hydrogels as In Vitro Models and Cell Delivery Systems

Arthi Shridhar, Elizabeth Gillies, Brian G. Amsden, and Lauren E. Flynn

19. 3D-Printing Composite Polycaprolactone-Decellularized Bone Matrix Scaffolds for Bone Tissue Engineering Applications

Alexandra N. Rindone, Ethan Nyberg, and Warren L. Grayson

20. Decellularization of Large Tendon Specimens: Combination of Manually Performed Freez

This practical, hands-on volume examines the use of decellularized tissues and organs as biologically-active scaffolds by providing numerous approaches from experts in the field. While knowledge of how to grow and differentiate cells in culture has dramatically improved over time, the book addresses the challenges of how to instruct particular cells of interest to recognize and respond to their environment so as to proliferate, differentiate, and function for restoration of original tissue and organ form and function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.