Carbohydrate Microarrays : Methods and Protocols

1. Recent Advances and Future Challenges in Glycan Microarray Technology

José L. de Paz and Peter Seeberger

2. Chemical Synthesis of Carbohydrates and Their Surface Immobilisation: A Brief Introduction

Daniel B. Werz

3. General Consideration on Sialic Acid Chemistry

Hongzhi Cao and Xi Chen

4. Synthesis of Azido-Functionalized Carbohydrates for the Design of Glycoconjugates

Samy Cecioni, David Goyard, Jean-Pierre Praly, and Sébastian Vidal

5. Polypyrrole-Oligosaccharide Microarray for the Measurement of Biomolecular Interactions by Surface Plasmon Resonance Imaging

Julia Bartoli, André Roget, and Thierry Livache

6. Glycosylated Self-Assembled Monolayers for Arrays and Surface Analysis

Fang Cheng and Daniel M. Ratner

7. Carbohydrate Microarrays for Enzymatic Reactions and Quantification of Binding Affinities for Glycan-Protein Interactions

Myung-Ryul Lee, Sungjin Park, and Injae Shin

8. Neoglycolipid (NGL)-Based Oligosaccharide Microarray System: Preparation of NGLs and Their Non-Covalent Immobilization on Nitrocellulose-Coated Glass Slides for Microarray Analyses

Yan Liu, Robert A. Childs, Angelina S. Palma, Maria A. Campanero-Rhodes, Mark S. Stoll, Wengang Chai, and Ten Feizi

9. Preparation of a Mannose-6-phosphate Glycan Microarray Through Fluorescent Derivatization, Phosphorylation, and Immobilization of Natural High-mannose N-glycans and its Application in Ligand Identification of P-type Lectins

Xuezheng Song, Jamie Heimburg-Molinaro, Nancy M. Dahms, David F. Smith, and Richard D. Cummings

10. Production of Fluorous-Based Microarrays with Uncharged Carbohydrates

Sahana K. Nagappayya and Nicola L. B. Pohl

11. General Procedure for the Synthesis of Neoglycoproteins and Immobilization on Epoxide-Modified Glass Slides

Yalong Zhang and Jeffrey C. Gildersleeve

12. Immobilization of Polyacrylamide-Based Glycoconjugates on Solid Phase in Immunosorbent Assays

Oxana E. Galanina, Alexander A. Chinarev, Hadezha V. Shilova, Marina A. Sablina, and Nicolai V. Bovin

13. Surface Plasmon Resonance Imaging Analysis of Protein Binding to a Sialoside-Based Carbohydrate Microarray

Matthew J. Linman, Hai Yu, Xi Chen, Quan Cheng

14. Glycoarray by DNA Directed Immobilization

Yann Chevolot, Jing Zhang, Albert Meyer, Sébastien Vidal, Jean-Pierre Praly, Jean-Jacques Vasseur, Eliane Souteyrand, and François Morvan

15. Fabrication of Carbohydrate Surfaces by Using Non Derivatised Oligosaccharides

Jonathan Popplewell, Marcus Swan, Gavin Brown, and Bob Lauder

16. Polysaccharide Microarrays. Application to the Identification of Heparan Sulfate Mimetics

Justin Dheur, Nabil Dendane, Rémi Desmet, Fatima Dahmani, Gauthier Goormachtigh, Jérome Vicogne, Véronique Fafeur, Oleg Melnyk

17. Carbohydrate Antigen Microarrays

Denong Wang

18. Probing Virus-Glycan Interactions Using Glycan Microarrays

Jamie Heimburg-Molinaro, Mary Tappert, Xuezheng Song, Yi Lasanajak, Gillian Air, David F. Smith, and Richard D. Cummings

19. MALDI-ToF MS Analysis of Glycosyltransferase Activities on Gold Surface Arrays

Nicolas Laurent, Rose Haddoub, Josef Voglmeir, and Sabine L. Flitsch

20. Microarray Technology using Glycans Extracted from Natural Sources for Serum Antibody Fluorescent Detection

Emanuela Lonardi, André M. Deelder, Manfred Wuhrer, and Crina I.A. Balog

21. Studying Modification of Aminoglycoside Antibiotics by Resistance-causing

Carbohydrate microarrays emerged as a key technology for the deciphering of the glycospace by providing a multiplex technology where tens to hundreds of carbohydrates/protein interactions can be probed in parallel. Carbohydrate Microarrays: Methods and Protocols aims to give the reader the theoretical and experimental clues necessary for the fabrication and implementation of carbohydrate microarrays. This requires three essential steps: 1) to obtain the carbohydrate probes (monosacharides, oligosacchrides, polysacchairdes, glycoconjugates or glycoclusters), 2) to immobilize these probes, and 3) to implement the protocols for biological/biochemical interaction with the desired target. This volume gives an overview of carbohydrate microarray and carbohydrate chemistry and illustrates different detection techniques and their applications. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Carbohydrate Microarrays: Methods and Protocols compiles a catalogue of protocols on carbohydrate microarrays to span the needs of researchers around the globe.