1. Recent Advances and Future Challenges in Glycan Microarray Technology
José L. de Paz and Peter Seeberger
2. Chemical Synthesis of Carbohydrates and Their Surface Immobilisation: A Brief Introduction
Daniel B. Werz
3. General Consideration on Sialic Acid Chemistry
Hongzhi Cao and Xi Chen
4. Synthesis of Azido-Functionalized Carbohydrates for the Design of Glycoconjugates
Samy Cecioni, David Goyard, Jean-Pierre Praly, and Sébastian Vidal
5. Polypyrrole-Oligosaccharide Microarray for the Measurement of Biomolecular Interactions by Surface Plasmon Resonance Imaging
Julia Bartoli, André Roget, and Thierry Livache
6. Glycosylated Self-Assembled Monolayers for Arrays and Surface Analysis
Fang Cheng and Daniel M. Ratner
7. Carbohydrate Microarrays for Enzymatic Reactions and Quantification of Binding Affinities for Glycan-Protein Interactions
Myung-Ryul Lee, Sungjin Park, and Injae Shin
8. Neoglycolipid (NGL)-Based Oligosaccharide Microarray System: Preparation of NGLs and Their Non-Covalent Immobilization on Nitrocellulose-Coated Glass Slides for Microarray Analyses
Yan Liu, Robert A. Childs, Angelina S. Palma, Maria A. Campanero-Rhodes, Mark S. Stoll, Wengang Chai, and Ten Feizi
9. Preparation of a Mannose-6-phosphate Glycan Microarray Through Fluorescent Derivatization, Phosphorylation, and Immobilization of Natural High-mannose N-glycans and its Application in Ligand Identification of P-type Lectins
Xuezheng Song, Jamie Heimburg-Molinaro, Nancy M. Dahms, David F. Smith, and Richard D. Cummings
10. Production of Fluorous-Based Microarrays with Uncharged Carbohydrates
Sahana K. Nagappayya and Nicola L. B. Pohl
11. General Procedure for the Synthesis of Neoglycoproteins and Immobilization on Epoxide-Modified Glass Slides
Yalong Zhang and Jeffrey C. Gildersleeve
12. Immobilization of Polyacrylamide-Based Glycoconjugates on Solid Phase in Immunosorbent Assays
Oxana E. Galanina, Alexander A. Chinarev, Hadezha V. Shilova, Marina A. Sablina, and Nicolai V. Bovin
13. Surface Plasmon Resonance Imaging Analysis of Protein Binding to a Sialoside-Based Carbohydrate Microarray
Matthew J. Linman, Hai Yu, Xi Chen, Quan Cheng
14. Glycoarray by DNA Directed Immobilization
Yann Chevolot, Jing Zhang, Albert Meyer, Sébastien Vidal, Jean-Pierre Praly, Jean-Jacques Vasseur, Eliane Souteyrand, and François Morvan
15. Fabrication of Carbohydrate Surfaces by Using Non Derivatised Oligosaccharides
Jonathan Popplewell, Marcus Swan, Gavin Brown, and Bob Lauder
16. Polysaccharide Microarrays. Application to the Identification of Heparan Sulfate Mimetics
Justin Dheur, Nabil Dendane, Rémi Desmet, Fatima Dahmani, Gauthier Goormachtigh, Jérome Vicogne, Véronique Fafeur, Oleg Melnyk
17. Carbohydrate Antigen Microarrays
Denong Wang
18. Probing Virus-Glycan Interactions Using Glycan Microarrays
Jamie Heimburg-Molinaro, Mary Tappert, Xuezheng Song, Yi Lasanajak, Gillian Air, David F. Smith, and Richard D. Cummings
19. MALDI-ToF MS Analysis of Glycosyltransferase Activities on Gold Surface Arrays
Nicolas Laurent, Rose Haddoub, Josef Voglmeir, and Sabine L. Flitsch
20. Microarray Technology using Glycans Extracted from Natural Sources for Serum Antibody Fluorescent Detection
Emanuela Lonardi, André M. Deelder, Manfred Wuhrer, and Crina I.A. Balog
21. Studying Modification of Aminoglycoside Antibiotics by Resistance-causing
Carbohydrate microarrays emerged as a key technology for the deciphering of the glycospace by providing a multiplex technology where tens to hundreds of carbohydrates/protein interactions can be probed in parallel. Carbohydrate Microarrays: Methods and Protocols aims to give the reader the theoretical and experimental clues necessary for the fabrication and implementation of carbohydrate microarrays. This requires three essential steps: 1) to obtain the carbohydrate probes (monosacharides, oligosacchrides, polysacchairdes, glycoconjugates or glycoclusters), 2) to immobilize these probes, and 3) to implement the protocols for biological/biochemical interaction with the desired target. This volume gives an overview of carbohydrate microarray and carbohydrate chemistry and illustrates different detection techniques and their applications. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Carbohydrate Microarrays: Methods and Protocols compiles a catalogue of protocols on carbohydrate microarrays to span the needs of researchers around the globe.
