CRISPR-Cas Systems for RNA and Genome Editing (Volume 712) (Methods in Enzymology, Volume 712)

CRISPR-Cas Systems for RNA and Genome Editing, Volume 711 in this ongoing series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Massively parallel genomic perturbations with multi-target CRISPR interrogates Cas9 activity and DNA repair at endogenous sites, Genome oligopaint via local denaturation fluorescence in situ hybridization, Biochemical reconstitution of a type I-B CRISPR-associated transposon, Visualizing the conformational landscape of CRISPR-Cas9 through kinetics-informed structural studies, and much more. Additional sections cover Analysis of metal-dependent DNA nicking activities by Cas endonucleases, Biochemically characterizing the accessory proteins and ribonucleoprotein complexes of Type IV CRISPR systems, Prime editing in bacteria with BacPE, Preparation of high-purity CRISPR-based DNA base editor RNPs, Bacterial directed evolution of CRISPR base editors, Cloning and validating systems for high throughput molecular recording, Using CRISPR-Cas13 for viral nucleic acid detection, and more.