Bioluminescence: Methods and Protocols (Methods in Molecular Biology)

A state-of-the-art collection of innovative methods for the use of luciferase and green fluorescent protein in analytical biochemistry, molecular toxicology, environmental monitoring, and cell-based assays. The contributors - all hands-on researchers who have perfected their protocols to a high degree of reproducibility - illustrate these powerful methods with a wide variety of applications ranging from the analytical determination (titer) of specific molecules to the cellular detection of a wide range of pollutants, to microbiological detection and analysis, and the molecular biological manipulation of lux, luc, and gfp structural genes. Step-by-step methods are also included for the construction of other recombinant organisms.