Application of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics (Paperback)

Idioma: inglés

Editorial: Springer-Verlag New York Inc., New York, NY, 2013

1461486653 / 9781461486657

Serie: Libro 5 de 12 - SpringerBriefs in Systems Biology

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Paperback. A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions. The method that is most widely used is the Western blot analysis. While Western blot is a workhorse in laboratories studying protein expression and has several advantages, it also has a number of significant limitations. In particular, the method is semi-quantitative with limited dynamic range. Western blot focuses on a single protein per sample with only a small number of representative samples analyzed in an experiment. New quantitative tools have been needed for some time to at least supplement, & possibly replace, the Western blot. Mass spectrometric methods have begun to compete with Western blot for routine quantitative analyses of proteins. One of these methods is based on the tandem mass spectrometry technique of selected reaction monitoring (SRM), which is also called multiple reaction monitoring (MRM). Selected reaction monitoring is actually an older tandem mass spectrometry technique, first described in the late 70s, that is widely utilized in the quantitative analysis of small molecules like drugs & metabolites. The use of selected reaction monitoring for the quantitative analysis of proteins has a number of advantages. Most importantly, it is fundamentally quantitative with a wide dynamic range. The output of the analysis is a numerical result that can range over several orders of magnitude. Other advantages include sufficient specificity & sensitivity to detect low abundance proteins in complex mixtures. Finally, selected reaction monitoring can be multiplexed to allow the quantitative analysis of relatively large numbers of proteins in a single sample in a single experiment. This Brief will explain both the theoretical & experimental details of the selected reaction monitoring experiment as it is appliedto proteins. A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

N° de ref. del artículo 9781461486657

Título
Application of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics (Paperback)
Autor
Michael Kinter
Editorial
Springer-Verlag New York Inc., New York, NY
Año de publicación
2013
Estado
new
Encuadernación
Paperback
Idioma
inglés
ISBN 10
1461486653
ISBN 13
9781461486657
Serie
Libro 5 de 12: SpringerBriefs in Systems Biology

Grand Eagle Retail

Bensenville, IL, Estados Unidos de America

Vendedor de 5 estrellas

Vendedor de AbeBooks desde 12 de octubre de 2005

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