Androgen Action : Methods and Protocols

Part I. Reviews

1. Molecular Mechanisms of Androgen Action - A Historical Perspective

Albert O. Brinkmann

2. Androgen Action during Prostate Carcinogenesis

Diping Wang and Donald J. Tindall

Part II. Analysis of Androgens

3. Androgen Quantitation in Prostate Cancer Tissue using Liquid Chromatography Tandem Mass Spectrometry

Mark Titus and Kenneth B. Tome

4. Ligand Competition Binding Assay for the Androgen Receptor

Clémentine Féau, Leggy A. Arnold, Aaron Kosinski, and R. Kiplin Guy

Part III. Androgen receptor biology

5. Analysis of Androgen Receptor Activity by Reporter Gene Assays

Harri Makkonen, Tiina Jääskeläinen, Miia Rytinki, and Jorma J. Palvimo

6. Identification and Characterization of Androgen Response Elements

Reinhilde De Bruyn, Rita Bollen, and Frank Claessens

7. In vitro and In vivo Silencing of the Androgen Receptor

Helen Cheng, Eric Leblanc, and Paul S. Rennie

8. Analysis of Interdomain Interactions of the Androgen Receptor

Elizabeth M. Wilson

9. Methods to Study Dynamic Interaction of Androgen Receptor with Chromatin in Living Cells

Hatice Zeynep Kirli and Fahri Saatcioglu

10. FRET Analysis of Androgen Receptor Structure and Biochemistry in Living Cells

Fred Schaufele

Part IV. Analysis of Androgen Receptor Modifications and Interactions

11. Analysis of Nuclear Receptor Acetylation

Chenguang Wang, Michael Powell, Lifeng Tian, and Richard G. Pestell

12. Analysis of Androgen Receptor SUMOylation

Miia M. Rytinki, Sanna Kaikkonen, Päivi Sutinen, and Jorma J. Palvimo

13. Analysis of Ligand-specific Co-repressor Binding to the Androgen Receptor

Claudia Gerlach, Daniela Roell, and Aria Baniahmad

14. Detection of Ligand-selective Interactions of the Human Androgen Receptor by SELDI-MS-TOF

Thomas Linke, Martin Scholten, and Aria Baniahmad

Part V. Recent Advances in Studying Androgen Receptor Signalling

15. Global Identification of Androgen Response Elements

Charles E. Massie and Ian G. Mills

16. Tissue Specific Knockout of Androgen Receptor in Mice

Tzuhua Lin, Shuyuan Yeh, and Chawnshang Chang

17. Methodology to Investigate Androgen-sensitive and Castration-resistant Human Prostate Cancer Xenografts in Preclinical Setting

Holly M. Nguyen and Eva Corey

18. Automated Microscopy and Image Analysis for Androgen Receptor Function

Sean M. Hartig, Justin Y. Newberg, Michael J. Bolt, Adam T. Szafran, Marco Marcelli, and Michael A. Mancini

Part VI. Cross-talk of Androgen and Other Signalling Pathways

19. Androgen Regulation of ETS-gene Fusion Transcripts in Prostate Cancer

Delila Gasi and Jan Trapman

20. Regulation of Apoptosis by Androgens in Prostate Cancer Cells

Yke Jildouw Arnoldussen, Ling Wang, and Fahri Saatcioglu

21. Analysis of Androgen Receptor Rapid Actions in Cellular Signaling Pathways: Receptor /Src Association

Antimo Migliaccio, Gabriella Castoria, and Ferdinando Auricchio

22. Analysis of Androgen-induced Increase in Lipid Accumulation in Prostate Cancer Cells

Jørgen Sikkeland, Torstein Lindstad, and Fahri Saatcioglu

Androgens play a critical role in the development and maintenance of the male reproductive system and affect important physiological processes and pathological conditions, including the homeostasis of the normal prostate and prostate cancer. Androgen Action: Methods and Protocols is designed to provide a tool box to study various phases of androgen action, from its entry to the cell to the phenotypic response that the cell mounts, with up-to-date techniques for biochemists, molecular biologists, cell biologists, geneticists, and pathologists. The volume opens with a brief review of the research history on androgen action and prostate carcinogenesis, followed by chapters that cover state-of-the-art methods to determine androgen levels in biological tissues and fluids, experimental procedures to study the various aspects of androgen receptor activity, and methodology to study salient examples of interactions between androgen signalling and other major signalling pathways in cells. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Androgen Action: Methods and Protocols provides a comprehensive overview of, and practical guidance on, the diverse methodologies that are propelling androgen action research forward, both for normal physiology as well as in disease states.