Applications of Continuous and Steady-State Methods to Root Biology: 34 (Developments in Plant and Soil Sciences) - Tapa blanda

 
9789401075022: Applications of Continuous and Steady-State Methods to Root Biology: 34 (Developments in Plant and Soil Sciences)
Tapa blanda
ISBN 10: 9401075026 ISBN 13: 9789401075022
Editorial: Springer, 2011

Sinopsis

This book provides an excellent illustration of the interrelationship between progress in scientific methodology and conceptual advances, and its publica­ tion should contribute to further advances. It is well known that major advances in understanding often follow the development of new methods. The development of the acetylene reduction assay for nitrogenase activity provides a good example of this interrelationship between theory and methods. Theoretical knowledge led to a search for substrates for nitro­ genase that could be assayed for more easily than ammonium, the normal product of the enzyme. The discovery of the reduction of acetylene to ethylene by nitrogenase provided the ideal answer to the problem by provid­ ing a rapid, specific, nondestructive, and inexpensive assay for nitrogenase activity. This assay is now used by almost every laboratory doing research on nitrogen fixation. However, further use and development of the acetylene reduction assay has shown that it can underestimate nitrogenase activity and can even give incorrect relative values under some circumstances. The major problem is that exposure of legume nodules to acetylene can cause a large increase in the resistance to oxygen diffusion into the nodule. This reduced supply of oxygen decreases the rate of nitrogenase activity within a few minutes.

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Críticas

` This book deserves to be purchased by every library and institute as the most uptodate reference work on continuous and steady-state methods and their application to root biology. '
A. Ekbote, Indian Journal of Agricultural Sciences, Vol. 60, no. 4

Reseña del editor

This book provides an excellent illustration of the interrelationship between progress in scientific methodology and conceptual advances, and its publica­ tion should contribute to further advances. It is well known that major advances in understanding often follow the development of new methods. The development of the acetylene reduction assay for nitrogenase activity provides a good example of this interrelationship between theory and methods. Theoretical knowledge led to a search for substrates for nitro­ genase that could be assayed for more easily than ammonium, the normal product of the enzyme. The discovery of the reduction of acetylene to ethylene by nitrogenase provided the ideal answer to the problem by provid­ ing a rapid, specific, nondestructive, and inexpensive assay for nitrogenase activity. This assay is now used by almost every laboratory doing research on nitrogen fixation. However, further use and development of the acetylene reduction assay has shown that it can underestimate nitrogenase activity and can even give incorrect relative values under some circumstances. The major problem is that exposure of legume nodules to acetylene can cause a large increase in the resistance to oxygen diffusion into the nodule. This reduced supply of oxygen decreases the rate of nitrogenase activity within a few minutes.

"Sobre este título" puede pertenecer a otra edición de este libro.

Editorial
Springer
Año de publicación
2011
Idioma
Inglés
ISBN 10 
9401075026
ISBN 13 
9789401075022
Encuadernación
Tapa blanda
Número de páginas
268
Editor
Torrey John G., Winship Lawrence J.
Contacto del fabricante
no disponible
Persona responsable
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Otras ediciones populares con el mismo título

9780792300243: Applications of Continuous and Steady-State Methods to Root Biology: 34 (Developments in Plant and Soil Sciences, 34)

Edición Destacada

ISBN 10:  0792300246 ISBN 13:  9780792300243
Editorial: Springer, 1989
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