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Characterization of Pasteurella multocida: Pasteurella multocida, the causative agent of Hemorrhagic Septicemia - Tapa blanda

Durrani, Rabia; Fawad Ali Khan, .; Naeem Ali, .

 
9783846583197: Characterization of Pasteurella multocida: Pasteurella multocida, the causative agent of Hemorrhagic Septicemia
Tapa blanda
ISBN 10: 3846583197 ISBN 13: 9783846583197
Editorial: LAP LAMBERT Academic Publishing, 2012

Sinopsis

Hemorrhagic septicemia has been an acute and fatal disease among cattle and buffaloes caused by Pasteurella multocida. The prevalence of this disease is 49% in animals of Pakistan. The present study was to evaluate the prevalence, multidrug resistance and the molecular heterogeneity within clinical strains of P. multocida collected from different cities of Pakistan. Tissue samples of lung and spleen of dead animals were used for diagnostics. Rapid plate screening test for Hyaluronidase of clinical Isolates showing clear and white zones proved that given isolate is pathogenic. Amongst one hundred and ninety nine total isolates, only one hundred and one isolates were found to be virulent as clinical isolate of P. multocida. Isolates were confirmed through amplification (PCR) of specie–specific and Hemorrhagic septicemia type B causing test. The primer pair of specie –specific test showed a band of 300bp in all subspecies of P.multocida whereas the primer pair of HS type B causing test showed a band of 460bp in all HS-causing serotype of P.multocida. The high prevalence was found only in Karachi. Whole cell and envelope protein profile of the isolates on SDS-PAGE showed presence of

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Reseña del editor

Hemorrhagic septicemia has been an acute and fatal disease among cattle and buffaloes caused by Pasteurella multocida. The prevalence of this disease is 49% in animals of Pakistan. The present study was to evaluate the prevalence, multidrug resistance and the molecular heterogeneity within clinical strains of P. multocida collected from different cities of Pakistan. Tissue samples of lung and spleen of dead animals were used for diagnostics. Rapid plate screening test for Hyaluronidase of clinical Isolates showing clear and white zones proved that given isolate is pathogenic. Amongst one hundred and ninety nine total isolates, only one hundred and one isolates were found to be virulent as clinical isolate of P. multocida. Isolates were confirmed through amplification (PCR) of specie-specific and Hemorrhagic septicemia type B causing test. The primer pair of specie -specific test showed a band of 300bp in all subspecies of P.multocida whereas the primer pair of HS type B causing test showed a band of 460bp in all HS-causing serotype of P.multocida. The high prevalence was found only in Karachi. Whole cell and envelope protein profile of the isolates on SDS-PAGE showed presence of

Biografía del autor

BS(Hons) Microbiology Kohat University Of Science and Technology KPK PakistanMasters Microbiology Quaid-I-Azam University Islamabad Pakistan.Fawad Ali Khan BS(Hons) Biotechnology University of Malakand Dir Lower KPK PakistanNaeem Ali PhD Microbiology (Quaid-I-Azam University Islamabad).

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Editorial
LAP LAMBERT Academic Publishing
Año de publicación
2012
Idioma
Inglés
ISBN 10 
3846583197
ISBN 13 
9783846583197
Encuadernación
Tapa blanda
Número de páginas
84
Contacto del fabricante
no disponible
Persona responsable
no disponible