A Laboratory Guide to Glycoconjugate Analysis: v. 9 (Biomethods) - Tapa dura

9783764352103: A Laboratory Guide to Glycoconjugate Analysis: v. 9 (Biomethods)

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ISBN 10: 3764352108 ISBN 13: 9783764352103

Editorial: Birkhauser Verlag AG, 1997

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18. 2 Principle of FACE/Gel Retardation Assay ...349 18. 3 Labelling of Oligosaccharides with ANTS ...350 18. 4 Screening of Carbohydrate Ligands for Proteins ...352 18. 5 Measurement of Binding Constant for the Interaction Between Protein and ANTS-Labelled Carbohydrate ...355 18. 6 Measurement of Binding Constant for the Interaction Between Protein and Native Carbohydrate ...357 References ...360 ~ The Application of Capillary Affinity Electrophoresis to the Analysis _ of Carbohydrate-Protein Interactions ...361 19. 1 Introduction ...361 19. 2 Principle of CAE ...363 19. 3 Determination of Association Constants ...364 19. 4 Technical Procedures ...366 General considerations ...366 19. 5 Limitations of the Technique ...370 19. 6 Application of CAE to the Analysis of Carbohydrate-Protein Interactions ...371 19. 7 Conclusions ...375 References ...377 20. 1 Introduction ...379 Definitions ...380 20. 2 Technical Procedures ...381 20. 3 Sample Detection and Sample Recovery ...389 Autoradiography and staining ...389 Sample detection by blotting ...389 Semipreparative ACE ...390 20. 4 Analysis of Data ...391 Measuring sample mobilities - calculating a retardation coefficient ...3 91 Graphical analysis of data ...392 Interpreting ACE patterns ...393 Reverse ACE ...395 20. 5 Summary ...397 Acknowledgements ...398 References ...398 Subject Index ...399 XII List of Contributors Nebojsa Avdalovic John T. Gallagher Dionex Corporation Cancer Research Campaign Department of Medical Oncology 445 Lakeside Drive University of Manchester Sunnyvale, CA 94086 Christie CRC Research Centre Klaus Biemann Wilmslow Road Department of Chemistry Manchester M20 4BX Massachusetts Institute of Technology UK Cambridge, MA 02139-4307 USA Geoffrey R.

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Rese�a del editor

18. 2 Principle of FACE/Gel Retardation Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 349 18. 3 Labelling of Oligosaccharides with ANTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 350 18. 4 Screening of Carbohydrate Ligands for Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . 352 18. 5 Measurement of Binding Constant for the Interaction Between Protein and ANTS-Labelled Carbohydrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 355 18. 6 Measurement of Binding Constant for the Interaction Between Protein and Native Carbohydrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 357 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 360 ~ The Application of Capillary Affinity Electrophoresis to the Analysis _ of Carbohydrate-Protein Interactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361 19. 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361 19. 2 Principle of CAE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 363 19. 3 Determination of Association Constants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 364 19. 4 Technical Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366 General considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366 19. 5 Limitations of the Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 370 19. 6 Application of CAE to the Analysis of Carbohydrate-Protein Interactions . . . . . . 371 19. 7 Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 375 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 377 20. 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 379 Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 380 20. 2 Technical Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 381 20. 3 Sample Detection and Sample Recovery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Autoradiography and staining . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Sample detection by blotting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Semipreparative ACE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 390 20. 4 Analysis of Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 391 Measuring sample mobilities - calculating a retardation coefficient . . . . . . . . . . . . 391 Graphical analysis of data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 392 Interpreting ACE patterns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 393 Reverse ACE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 395 20. 5 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 397 Acknowledgements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 398 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 398 Subject Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 399 XII List of Contributors Nebojsa Avdalovic John T. Gallagher Dionex Corporation Cancer Research Campaign Department of Medical Oncology 445 Lakeside Drive University of Manchester Sunnyvale, CA 94086 Christie CRC Research Centre Klaus Biemann Wilmslow Road Department of Chemistry Manchester M20 4BX Massachusetts Institute of Technology UK Cambridge, MA 02139-4307 USA Geoffrey R.

Rese�a del editor

This book is an up-to-date compendium that describes in detail methods for the analysis of complex carbohydrates that occur in biological systems. It has a broad scope including a variety of new methods both for glycans and glycosaminoglycans. The text includes methods for oligosaccharide analysis (glycoprotein detection and blotting, composition, mass spectrometry), profiling (electrophoretic and chromatographic), sequencing using enzymes (electrophoretic, chromatographic and mass spectrometric methods), glycan identification (electrophoretic or chromatographic positional matching), glycan derivatization and the analysis of molecular interactions of glycans and glycosaminoglycans. The format of this text is such that it is suitable for use by glycobiologists including established researchers, research students and workers new to the field, in academic or commercial laboratories.

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EditorialBirkhauser Verlag AG
A�o de publicaci�n1997
ISBN 10 3764352108
ISBN 13 9783764352103
Encuadernaci�nTapa dura
IdiomaIngl�s
N�mero de p�ginas432
EditorJackson Professor Peter, Gallagher J. T.
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Condici�n: Bien

XXII, 405 pages with ill. and fig...

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A Laboratory Guide to Glycoconjugate Analysis. (= Biomethods, Vol. 9).

Jackson, Peter and J.T. Gallagher (Eds.):

Publicado por Basel, Boston, Berlin: Birkh�user, 1997

ISBN 10: 3764352108 ISBN 13: 9783764352103

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Librería: Versandantiquariat Waffel-Schr�der, Berlin, Alemania

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Hardcover/Pappeinband. Condici�n: Gut. XXII, 405 pages with ill. and fig.; 24 cm. Good. Former library copy with usual markings. Apart from that inside clean. Cover worn. Sprache: Englisch Gewicht in Gramm: 1350. N� de ref. del art�culo: 24719

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