ZebraTrack: An Automated Method to Study Zebrafish Behaviour - Tapa blanda

Bhargava, Yogesh; Bhargava, Anamika

 
9783659900518: ZebraTrack: An Automated Method to Study Zebrafish Behaviour
Tapa blanda
ISBN 10: 3659900516 ISBN 13: 9783659900518
Editorial: LAP LAMBERT Academic Publishing, 2017

Sinopsis

Behavioural neuroscience relies on software driven methods for behavioural assessment. Therefore, we propose a novel method which we called as ZebraTrack. It includes cost-effective imaging setup for distraction-free behavioural acquisition, automated tracking using open-source ImageJ software and workflow for extraction of behavioural endpoints. Our ImageJ algorithm is capable of providing control to users at key steps while maintaining automation in tracking without the need for the installation of external plugins. We have validated this method by testing novelty induced anxiety behaviour in adult zebrafish. Our results, in agreement with established findings, showed that during state-anxiety, zebrafish showed reduced distance travelled, increased thigmotaxis and freezing events. Furthermore, we proposed a method to represent both spatial and temporal distribution of choice-based behaviour which is currently not possible to represent using simple videograms. ZebraTrack method is simple and economical, yet robust enough to give results comparable with those obtained from costly proprietary software like Ethovision XT.

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Reseña del editor

Behavioural neuroscience relies on software driven methods for behavioural assessment. Therefore, we propose a novel method which we called as ZebraTrack. It includes cost-effective imaging setup for distraction-free behavioural acquisition, automated tracking using open-source ImageJ software and workflow for extraction of behavioural endpoints. Our ImageJ algorithm is capable of providing control to users at key steps while maintaining automation in tracking without the need for the installation of external plugins. We have validated this method by testing novelty induced anxiety behaviour in adult zebrafish. Our results, in agreement with established findings, showed that during state-anxiety, zebrafish showed reduced distance travelled, increased thigmotaxis and freezing events. Furthermore, we proposed a method to represent both spatial and temporal distribution of choice-based behaviour which is currently not possible to represent using simple videograms. ZebraTrack method is simple and economical, yet robust enough to give results comparable with those obtained from costly proprietary software like Ethovision XT.

Biografía del autor

Dr. Yogesh Bhargava, PhD: Assistant Professor at Dr. Harisingh Gour Central University, Sagar, MP, India. Expert in Zebrafish Behavioural Neuroscience and interested in Technology development. Dr. Anamika Bhargava, PhD: Assistant Professor at Indian Institute of Technology, Hyderabad, India. Expert in Molecular Biology and Electrophysiology.

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Editorial
LAP LAMBERT Academic Publishing
Año de publicación
2017
Idioma
Inglés
ISBN 10 
3659900516
ISBN 13 
9783659900518
Encuadernación
Tapa blanda
Número de páginas
60
Contacto del fabricante
no disponible
Persona responsable
no disponible