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Simple DNA Extraction Methods from Human Blood: A Comparison of Phenol - Chloroform -Isoamyle, Saturated Sodium Chloride,Guanidine Hydrochloride and ... for DNA Extraction from Human Blood Samples - Tapa blanda

Ali, Samar

 
9783659536342: Simple DNA Extraction Methods from Human Blood: A Comparison of Phenol - Chloroform -Isoamyle, Saturated Sodium Chloride,Guanidine Hydrochloride and ... for DNA Extraction from Human Blood Samples
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ISBN 10: 3659536342 ISBN 13: 9783659536342
Editorial: LAP LAMBERT Academic Publishing, 2014

Sinopsis

This study was aimed to compare between four DNA extraction methods, including phenol-chloroform-isoamyle, guanidine hydrochloride, saturated sodium chloride, and chelex-100 using 50 blood samples collected from healthy volunteers. The comparison included DNA concentration, extracted DNA purity, cost of reagents per one sample, and time consuming. After a comprehensive analysis of all factors: salt extraction gave the maximum yield of DNA, it is saver and simpler than the other methods. Moreover, this method is reliable and inexpensive. But its purity is slightly low. Phenol-chloroform-isoamyl extraction method give reliable quantity and purity of the DNA extracted. But it takes a time and labor in obtaining pure extract. Moreover, its associated toxicities warrant a judicious use. Chelex-100 resin extractions gave a DNA quantity as well as phenol-chloroform- isoamyle extraction. It is save, simple, does not require any organic solvent, and takes very short time and a little possibility of cross contamination. But it is expensive. Guanidine hydrochloride extraction is an expensive method taking a long time with a low quantity and quality of the DNA obtained.

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Reseña del editor

This study was aimed to compare between four DNA extraction methods, including phenol-chloroform-isoamyle, guanidine hydrochloride, saturated sodium chloride, and chelex-100 using 50 blood samples collected from healthy volunteers. The comparison included DNA concentration, extracted DNA purity, cost of reagents per one sample, and time consuming. After a comprehensive analysis of all factors: salt extraction gave the maximum yield of DNA, it is saver and simpler than the other methods. Moreover, this method is reliable and inexpensive. But its purity is slightly low. Phenol-chloroform-isoamyl extraction method give reliable quantity and purity of the DNA extracted. But it takes a time and labor in obtaining pure extract. Moreover, its associated toxicities warrant a judicious use. Chelex-100 resin extractions gave a DNA quantity as well as phenol-chloroform- isoamyle extraction. It is save, simple, does not require any organic solvent, and takes very short time and a little possibility of cross contamination. But it is expensive. Guanidine hydrochloride extraction is an expensive method taking a long time with a low quantity and quality of the DNA obtained.

Biografía del autor

Lecturer, Faculty of Medical Laboratory Sciences, Alneelain University, Khartoum, Sudan.M.Sc. in Parasitology and Medical Entomology - Alneelain University. (Jul-2011).Bachelor Degree in Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan (Oct-2004).

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Editorial
LAP LAMBERT Academic Publishing
Año de publicación
2014
Idioma
Inglés
ISBN 10 
3659536342
ISBN 13 
9783659536342
Encuadernación
Tapa blanda
Número de páginas
56
Contacto del fabricante
no disponible
Persona responsable
no disponible