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Quantitative DNA method in forensic DNA laboratory: Screening tool for efficient genotyping - Tapa blanda

Koljensic, Ivana

 
9783659415807: Quantitative DNA method in forensic DNA laboratory: Screening tool for efficient genotyping
Tapa blanda
ISBN 10: 3659415804 ISBN 13: 9783659415807
Editorial: LAP LAMBERT Academic Publishing, 2013

Sinopsis

Quantification of human DNA in forensic samples is an important step during STR profiling because the STR genotyping is sensitive to the quantity of DNA used in the PCR reaction.This study focuses on the importance of quantification in the entire process of genetic analysis.Two real time PCR platforms were used to compare two commercial kits in terms of DNA quantification.It was found out that accuracy of absolute quantification values in commercial quantification kits is strongly dependent on the construction of calibration curve. Especially low template DNA samples were used to assess whether QuantifilerTM or Plexor® HY System can determinate a minimum quantification value(cut off value)below which STR profiles would consistently fail to be detected. The usage of Plexor® HY System enabled to determine the cut off quantification value more exactly probably due to different molecular background and chemistry used in this kit.Reliability and other issues connected with cut off value are discussed. In order to better understand the relationship between the quantity of DNA and the number of detectable loci series the dilution expexperiment with standard DNA007 was done.

"Sinopsis" puede pertenecer a otra edición de este libro.

Reseña del editor

Quantification of human DNA in forensic samples is an important step during STR profiling because the STR genotyping is sensitive to the quantity of DNA used in the PCR reaction.This study focuses on the importance of quantification in the entire process of genetic analysis.Two real time PCR platforms were used to compare two commercial kits in terms of DNA quantification.It was found out that accuracy of absolute quantification values in commercial quantification kits is strongly dependent on the construction of calibration curve. Especially low template DNA samples were used to assess whether QuantifilerTM or Plexor® HY System can determinate a minimum quantification value(cut off value)below which STR profiles would consistently fail to be detected. The usage of Plexor® HY System enabled to determine the cut off quantification value more exactly probably due to different molecular background and chemistry used in this kit.Reliability and other issues connected with cut off value are discussed. In order to better understand the relationship between the quantity of DNA and the number of detectable loci series the dilution expexperiment with standard DNA007 was done.

Biografía del autor

Ivana Koljensic graduated from Charles University in Prague with a specialization anthropology and human genetics. During the study have focused on forensic genetics. She is a member of the international student organizations. And sees her future in forensic sciences.

"Sobre este título" puede pertenecer a otra edición de este libro.

Editorial
LAP LAMBERT Academic Publishing
Año de publicación
2013
Idioma
Inglés
ISBN 10 
3659415804
ISBN 13 
9783659415807
Encuadernación
Tapa blanda
Número de páginas
88
Contacto del fabricante
no disponible
Persona responsable
no disponible