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Sinopsis
Phylogenetic relationship among local and exotic genotypes of Capsicum were elaborated through morphological characterization and molecular markers.Protocols for isolation of total genomic DNA and seed storage proteins were optimized for Capsicum genotypes. Four RAPD primers were used to amplify genomic DNA. Unweighted Pair Group of Arithmetic Means (UPGMA) estimates of Genetic distances (GD) using RAPD primers ranged from 0 to 100%. Total seed storage proteins profile of the genotypes using SDS-PAGE analysis showed high range of genetic distances i.e. from 0–100% among various comparisons.The data obtained from PCR and SDS-PAGE was also used to study phylogenetic relationship through constructing two independent dendrograms. Dendrogram based on DNA data showed that the twenty-five genotypes were clustered into four groups.No such grouping was visible in the dendrogram based on the SDS-PAGE. Hence it was concluded that DNA based data is more useful for phylogenetic elaboration of Capsicum genotypes. Further morphogenetic and taxonomic study is recommended for assessment of the exact taxonomic status of genotypes segregated into different groups.
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Phylogenetic relationship among local and exotic genotypes of Capsicum were elaborated through morphological characterization and molecular markers.Protocols for isolation of total genomic DNA and seed storage proteins were optimized for Capsicum genotypes. Four RAPD primers were used to amplify genomic DNA. Unweighted Pair Group of Arithmetic Means (UPGMA) estimates of Genetic distances (GD) using RAPD primers ranged from 0 to 100%. Total seed storage proteins profile of the genotypes using SDS-PAGE analysis showed high range of genetic distances i.e. from 0-100% among various comparisons.The data obtained from PCR and SDS-PAGE was also used to study phylogenetic relationship through constructing two independent dendrograms. Dendrogram based on DNA data showed that the twenty-five genotypes were clustered into four groups.No such grouping was visible in the dendrogram based on the SDS-PAGE. Hence it was concluded that DNA based data is more useful for phylogenetic elaboration of Capsicum genotypes. Further morphogenetic and taxonomic study is recommended for assessment of the exact taxonomic status of genotypes segregated into different groups.
Biografía del autor
Nazia Akbar PhD Scholar, Department of Genetics Hazara University, Mansehra.
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Genetic Characterization Of Capsicum Germplasm Using Molecular Markers
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LAP LAMBERT Academic Publishing Jul 2014, 2014
ISBN 10: 3659297380
ISBN 13: 9783659297380
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Taschenbuch. Condición: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Phylogenetic relationship among local and exotic genotypes of Capsicum were elaborated through morphological characterization and molecular markers.Protocols for isolation of total genomic DNA and seed storage proteins were optimized for Capsicum genotypes. Four RAPD primers were used to amplify genomic DNA. Unweighted Pair Group of Arithmetic Means (UPGMA) estimates of Genetic distances (GD) using RAPD primers ranged from 0 to 100%. Total seed storage proteins profile of the genotypes using SDS-PAGE analysis showed high range of genetic distances i.e. from 0 100% among various comparisons.The data obtained from PCR and SDS-PAGE was also used to study phylogenetic relationship through constructing two independent dendrograms. Dendrogram based on DNA data showed that the twenty-five genotypes were clustered into four groups.No such grouping was visible in the dendrogram based on the SDS-PAGE. Hence it was concluded that DNA based data is more useful for phylogenetic elaboration of Capsicum genotypes. Further morphogenetic and taxonomic study is recommended for assessment of the exact taxonomic status of genotypes segregated into different groups. 112 pp. Englisch. Nº de ref. del artículo: 9783659297380
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Genetic Characterization of Capsicum Germplasm Using Molecular Markers
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VDM Verlag Dr. Mueller Aktiengesellschaft & Co. KG, 2014
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Genetic Characterization of Capsicum Germplasm Using Molecular Markers
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VDM Verlag Dr. Mueller Aktiengesellschaft & Co. KG, 2014
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Genetic Characterization Of Capsicum Germplasm Using Molecular Markers
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LAP LAMBERT Academic Publishing, 2014
ISBN 10: 3659297380
ISBN 13: 9783659297380
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Condición: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Akbar NaziaNazia Akbar PhD Scholar, Department of Genetics Hazara University, Mansehra.Phylogenetic relationship among local and exotic genotypes of Capsicum were elaborated through morphological characterization and molecular ma. Nº de ref. del artículo: 5146611
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Genetic Characterization of Capsicum Germplasm Using Molecular Markers
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VDM Verlag Dr. Mueller Aktiengesellschaft & Co. KG, 2014
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ISBN 13: 9783659297380
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Genetic Characterization Of Capsicum Germplasm Using Molecular Markers
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ISBN 10: 3659297380
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Taschenbuch. Condición: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Phylogenetic relationship among local and exotic genotypes of Capsicum were elaborated through morphological characterization and molecular markers.Protocols for isolation of total genomic DNA and seed storage proteins were optimized for Capsicum genotypes. Four RAPD primers were used to amplify genomic DNA. Unweighted Pair Group of Arithmetic Means (UPGMA) estimates of Genetic distances (GD) using RAPD primers ranged from 0 to 100%. Total seed storage proteins profile of the genotypes using SDS-PAGE analysis showed high range of genetic distances i.e. from 0 100% among various comparisons.The data obtained from PCR and SDS-PAGE was also used to study phylogenetic relationship through constructing two independent dendrograms. Dendrogram based on DNA data showed that the twenty-five genotypes were clustered into four groups.No such grouping was visible in the dendrogram based on the SDS-PAGE. Hence it was concluded that DNA based data is more useful for phylogenetic elaboration of Capsicum genotypes. Further morphogenetic and taxonomic study is recommended for assessment of the exact taxonomic status of genotypes segregated into different groups. Nº de ref. del artículo: 9783659297380
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Genetic Characterization Of Capsicum Germplasm Using Molecular Markers
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LAP LAMBERT Academic Publishing, 2014
ISBN 10: 3659297380
ISBN 13: 9783659297380
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Librería: preigu, Osnabrück, Alemania
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Taschenbuch. Condición: Neu. Genetic Characterization Of Capsicum Germplasm Using Molecular Markers | Nazia Akbar (u. a.) | Taschenbuch | 112 S. | Englisch | 2014 | LAP LAMBERT Academic Publishing | EAN 9783659297380 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu. Nº de ref. del artículo: 105181716
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Genetic Characterization Of Capsicum Germplasm Using Molecular Markers
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LAP LAMBERT Academic Publishing Jul 2014, 2014
ISBN 10: 3659297380
ISBN 13: 9783659297380
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Librería: buchversandmimpf2000, Emtmannsberg, BAYE, Alemania
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Taschenbuch. Condición: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Phylogenetic relationship among local and exotic genotypes of Capsicum were elaborated through morphological characterization and molecular markers.Protocols for isolation of total genomic DNA and seed storage proteins were optimized for Capsicum genotypes. Four RAPD primers were used to amplify genomic DNA. Unweighted Pair Group of Arithmetic Means (UPGMA) estimates of Genetic distances (GD) using RAPD primers ranged from 0 to 100%. Total seed storage proteins profile of the genotypes using SDS-PAGE analysis showed high range of genetic distances i.e. from 0-100% among various comparisons.The data obtained from PCR and SDS-PAGE was also used to study phylogenetic relationship through constructing two independent dendrograms. Dendrogram based on DNA data showed that the twenty-five genotypes were clustered into four groups.No such grouping was visible in the dendrogram based on the SDS-PAGE. Hence it was concluded that DNA based data is more useful for phylogenetic elaboration of Capsicum genotypes. Further morphogenetic and taxonomic study is recommended for assessment of the exact taxonomic status of genotypes segregated into different groups.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 112 pp. Englisch. Nº de ref. del artículo: 9783659297380
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