Differential-Display Reverse Transcription-P.C.R. (D.D.R.T.-P.C.R.) (Springer Lab Manuals) - Tapa blanda

Colonna-Romano, Sergio

 
9783540632979: Differential-Display Reverse Transcription-P.C.R. (D.D.R.T.-P.C.R.) (Springer Lab Manuals)
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ISBN 10: 3540632972 ISBN 13: 9783540632979
Editorial: Springer-Verlag, 1998

Sinopsis

Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA.
Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.

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De la contraportada

Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA.
Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.

"Sobre este título" puede pertenecer a otra edición de este libro.

Editorial
Springer-Verlag
Año de publicación
1998
Idioma
Inglés
ISBN 10 
3540632972
ISBN 13 
9783540632979
Encuadernación
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Número de páginas
136
Contacto del fabricante
no disponible
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Otras ediciones populares con el mismo título

9783642804557: Differential-Display Reverse Transcription-PCR (DDRT-PCR)

Edición Destacada

ISBN 10:  3642804551 ISBN 13:  9783642804557
Editorial: Springer, 2011
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