Sinopsis
This review of all electron microscopical techniques currently used also provides some recent principal research reports. Their application to the study of fine structures of various groups of plant pathogens (fungi, bacteria, viruses, mycoplasma-like organisms, protozoa and nematodes) is described as well as host-pathogen and host-symbiont interactions. In recent years, new preparation and labelling techniques (cryofixation, immunolabelling) have been applied in various disciplines of research, and these yield further specific information on the nature of parasitic interactions as detectable in the electron microscope. The reader will obtain an overview of the modern technical possibilities of studying plant pathogens using an electron microscopical approach.
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Reseña del editor
Plants, fungi, and viruses were among the first biological objects studied with an electron microscope. One of the two first instruments built by Siemens was used by Helmut Ruska, a brother of Ernst Ruska, the pioneer in constructing electron microscopes. H. Ruska published numerous papers on different biological objects in 1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a new age in microscopy. The main problem was then as it still is today, to obtain an appropriate preparation of the specimen for observation in the electron microscope. Beam damage and specimen thickness were the first obstacles to be met. L. Marton in Brussels not only built his own instrument, but also made considerable progress in specimen preparation by introducing the impregnation of samples with heavy metals to obtain useful contrast. His pictures of the bird nest orchid root impregnated with osmium were revolutionary when published in 1934. It is not the place here to recall the different techniques which were developed in the subsequent years to attain the modern knowledge on the fine structure of plant cells and of different plant pathogens. The tremendous progress obtained with tobacco mosaic virus is reflected in the chapter by M. Wurtz on the fine structure of viruses in this Volume. New cytochemical and immunological techniques considerably surpass the morphological information obtained from the pathogens, especially at the host-parasite interface.
Reseña del editor
This review of all electron microscopical techniques currently used also provides some recent principal research reports. Their application to the study of fine structures of various groups of plant pathogens (fungi, bacteria, viruses, mycoplasma-like organisms, protozoa and nematodes) is described as well as host-pathogen and host-symbiont interactions. In recent years, new preparation and labelling techniques (cryofixation, immunolabelling) have been applied in various disciplines of research, and these yield further specific information on the nature of parasitic interactions as detectable in the electron microscope. The reader will obtain an overview of the modern technical possibilities of studying plant pathogens using an electron microscopical approach.
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Electron Microscopy of Plant Pathogens
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Springer-Verlag Berlin and Heidelberg GmbH & Co. K, 1990
ISBN 10: 354052777X
ISBN 13: 9783540527770
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Librería: Cotswold Internet Books, Cheltenham, Reino Unido
Calificación del vendedor: 5 de 5 estrellas
Text in English. Ex-university library with stamps on pastedowns and top/bottom page edges; remnant of label on rear free endpaper. Pages faintly yellowed but otherwise clean and tidy in tight binding. Used - Good. Good ex-lib hardback Used - Good. Good ex-lib hardback. Nº de ref. del artículo: BOOKS327474I
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