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Electron Probe Microanalysis: Applications in Biology and Medicine - Tapa dura

 
9783540508038: Electron Probe Microanalysis: Applications in Biology and Medicine
Tapa dura
ISBN 10: 3540508031 ISBN 13: 9783540508038
Editorial: Springer-Verlag Berlin and Heidelberg GmbH & Co. K, 1989

Reseña del editor

The aim of electron probe microanalysis of biological systems is to identify, localize, and quantify elements, mass, and water in cells and tissues. The method is based on the idea that all electrons and photons emerging from an electron beam irradiated specimen contain information on its structure and composition. In particular, energy spectroscopy of X-rays and electrons after interaction of the electron beam with the specimen is used for this purpose. However, the application of this method in biology and medicine has to overcome three specific problems: 1. The principle constituent of most cell samples is water. Since liquid water is not compatible with vacuum conditions in the electron microscope, specimens have to be prepared without disturbing the other components, in parti­ cular diffusible ions (elements). 2. Electron probe microanaly­ sis provides physical data on either dry specimens or fully hydrated, frozen specimens. This data usually has to be con­ verted into quantitative data meaningful to the cell biologist or physiologist. 3. Cells and tissues are not static but dynamic systems. Thus, for example, microanalysis of physiolo­ gical processes requires sampling techniques which are adapted to address specific biological or medical questions. During recent years, remarkable progress has been made to overcome these problems. Cryopreparation, image analysis, and electron energy loss spectroscopy are key areas which have solved some problems and offer promise for future improvements.

Reseña del editor

The purpose of Electron Probe Microanalysis of biological systems is to identify, localize, and quantify elements, mass, and water in cells and tissues. The method itself is based on the idea that all electrons and photons emerging from a specimen irradiated by an electron beam, contain information on its structure and composition. Seven contributions concentrate on progress and problems of data acquisition and evaluation in X-ray microanalysis and electron energy loss spectroscopy, whereas the main topics cover the application of electron probe microanalysis in biological research: intracellular element localization, studies of epithelial iron transport and of dynamic processes in cells. Two reviews consider the application of this method in medicine, particularly in pathology and diagnosis.

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Editorial
Springer-Verlag Berlin and Heidelberg GmbH & Co. K
Año de publicación
1989
Idioma
Inglés
ISBN 10 
3540508031
ISBN 13 
9783540508038
Encuadernación
Tapa dura
Número de páginas
338
Editor
Zierold Karl, Hagler Herbert K
Contacto del fabricante
no disponible
Persona responsable
no disponible