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9783527319411: Proteomics in Practice: A Guide to Successful Experimental Design

Sinopsis

STILL THE ONLY CONCISE PRACTICAL GUIDE TO LABORATORY EXPERIMENTS IN PROTEOMICS, THIS NEW EDITION NOW ALSO COVERS DIGE TECHNOLOGY AND LIQUID–CHROMATOGRAPHY, WHILE THE TROUBLESHOOTING SECTION HAS BEEN CONSIDERABLY EXTENDED. ADOPTING A PRACTICAL APPROACH, THE AUTHORS PRESENT THE RELEVANT TECHNIQUES AND EXPLAIN THE ROUTE TO SUCCESSFUL EXPERIMENTAL DESIGN AND OPTIMAL METHOD SELECTION. THEY COVER SUCH ELECTROPHORETIC TECHNIQUES AS ISOELECTRIC FOCUSING, SDS PAGE, 2–D PAGE, AND DIGE, AS WELL AS LIQUID–CHROMATOGRAPHY TECHNIQUES, SUCH AS ION EXCHANGE, AFFINITY CHROMATOGRAPHY AND REVERSED–PHASE HPLC. MASS–SPECTROMETRIC TECHNIQUES INCLUDE MALDI, ESI, AND FT ICR. GENEROUSLY ILLUSTRATED, PARTLY IN COLOR, THE BOOK ALSO FEATURES UPDATES OF PROTOCOLS AS WELL AS ANIMATIONS ILLUSTRATING CRUCIAL METHODOLOGICAL STEPS ON A COMPANION WEBSITE.

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Críticas

"This book will be a valuable addition to any laboratory engaged in proteomics and is highly recommended." ( Internet Journal of Genomics and Proteomics 2008)

Reseña del editor

A combined review, manual and reference for the successful analysis of proteins using the classical approach of 2-D gel electrophoresis, mass spectrometry and related sequence database inquiries. The first section, written in a textbook style, introduces the entire technology, while the second section represents a comprehensive laboratory manual spanning the full range of methods from sample preparation to protein identification. Alternative methods and procedures are only suggested for those cases where the "default" procedure would fail to deliver adequate results. The third section is a unique troubleshooting guide, designed to answer many of the frequently asked questions regarding proteome analysis. The final section contains a thorough reference list to guide interested readers towards further detail. Intended for all those wishing to go beyond the theoretical aspects of proteome analysis, this book is targeted at research groups within academia and industry, course instructors, research assistants and graduate students. Special feature: Updates of recipes as well as animations illustrating crucial methodological steps will be available on the Web at http://www.wiley-vch.de/books/info/3-527-30354-5

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  • EditorialBlackwell Verlag GmbH
  • Año de publicación2008
  • ISBN 10 3527319417
  • ISBN 13 9783527319411
  • EncuadernaciónTapa dura
  • IdiomaInglés
  • Número de edición2
  • Número de páginas502

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Westermeier, Reiner
Publicado por Wiley-Blackwell, 2008
ISBN 10: 3527319417 ISBN 13: 9783527319411
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Condición: Good. Your purchase helps support Sri Lankan Children's Charity 'The Rainbow Centre'. Ex-library, so some stamps and wear, but in good overall condition. Our donations to The Rainbow Centre have helped provide an education and a safe haven to hundreds of children who live in appalling conditions. Nº de ref. del artículo: Z1-I-028-01356

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Westermeier, Reiner, Naven, Tom, Höpker, Hans-Rudolf
Publicado por Wiley-Blackwell, 2008
ISBN 10: 3527319417 ISBN 13: 9783527319411
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