Enzyme Assays and Enzyme Profiling: High Throughput Screening, Genetic Selection and Fingerprinting - Tapa dura

Acerca del autor

1985 Jean-Louis Reymond obtained his diploma working with Professor Seebach at the ETH Zurich. He finished his thesis in the field of total synthesis in 1989 in the group of Professor Vogel. After being in the United States for two years at the Scripps Research Institute he became an assistant professor there. 1997 he returned to the University of Bern, Switzerland. His research interest is biocatalysis and in particular enzyme design, assays, profiling, fingerprints and inhibitors.

De la contraportada

Nowadays, the characterization of enzymes using simple assays is a key technology for finding the optimum enzymes for, among others, catalysis in enzymology and drug discovery. Thus, enzymes have become an everyday tool not only for biologists, but also for chemists, and the number of applications is now overwhelming.

Edited by one of the leading experts in the field, this book fills the need for a book presenting the most important methods for high-throughput screenings and functional characterization of enzymes. It adopts an interdisciplinary approach, making it indispensable for all those involved in this expanding field, and reflects the major advances made over the past few years.

From the contents:

Part I. The Chemistry of Enzyme Assays
*Chromogenic assay of hydrolases for activity and enantioselectivity
* High-throughput screening systems for assaying the enantioselectivity of enzymes
* High throughput screening methods developed for oxidoreductases
* Industrial perspective on assays

Part II. Enzyme assays and Genetic Selection
* Agar-plate based assays
* Genetic selection for biocatalysts
* Three-hybrid systems and in vivo selection
* Nucleic-acid based approaches on the screening of enzymes

Part III. Enzyme Profiling
* Fluorescent probes for lipolytic enzymes
* Enzyme fingerprinting
* Protease profiling
* Enzyme assays on chips

For biochemists, analytical, organic and catalytic chemists, and biotechnologists.