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Cells and Tissues (v. 3) (Methods of immunological analysis) - Tapa dura

 
9783527279081: Cells and Tissues (v. 3) (Methods of immunological analysis)

Sinopsis

This is the third in a 12-volume work which provides both experts and non-specialists with the means to carry out qualitative and quantitative immunoanalysis. The first three volumes in the series supply a practice-oriented introduction to immunological analysis. The remaining eight volumes provide laboratory sheets of immunoanalytical techniques in various fields of the life sciences, chemistry, and medicine. One or more reliable methods are described for each analyte or class of analytes, typifying alternative approaches. In this way, users will find specific methods for widely-measured analytes that can be implemented without modification. Moreover, they can extract soundly-based principles and experimental techniques on which to construct immunoassays for new or less familiar analytes. Topics covered include immunoglobulins, complement components, auto-antibodies, tumour-related antigens, and various hormones and blood proteins. Each chapter is designed to give scientists enough information to reproduce the technique described quickly and accurately.

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De la contraportada

Methods of Immunological Analysis Edited by R. F. Masseyeff, W. H. Albert, N. A. Staines Volume 3: The latest in the MIA series, which is a remarkable practice-oriented account of immunological analysis. Volumes 1, 2 and 3 provide an extraordinarily comprehensive and up-to-date depiction of methods of immunological analysis. Volume 1 deals predominantly with methodological aspects of immunoassay formats and systems. Volume 2 covers the pre-analytical phase and the production and characterization of reagents. Volume 3 is concerned with the approach to using immunological methods for the analysis of cells and tissues. In the first sections, the methods necessary for the isolation of cells from many different tissues are described, with the emphasis upon human cells. Moreover, the various approaches to the use of antibody-based methods in immunocytochemistry are delineated. Their comparative uses and performance and discussed inc critical detail. The last part of the volume considers the ways in which immune cells can be analysed by methods conventionally understood by immunologists, but not necessarily using antibodies. A brief summary of major topics in Volume 3:

  • preparation of cells and tissues
  • cell culture
  • cell fractionation and purification
  • methods of phenotype analysis
  • immunoanalysis of cell function
  • analysis of immune cell function

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