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Developmental Competence of In-vitro Matured Goat Oocytes: Studies on developmental competence of in-vitro matured goat oocyetes in a medium with different supplementation - Tapa blanda

Singh, Kaushlendra Pratap; S.D. Kharche, Atul Saxena

 
9783330025752: Developmental Competence of In-vitro Matured Goat Oocytes: Studies on developmental competence of in-vitro matured goat oocyetes in a medium with different supplementation
Tapa blanda
ISBN 10: 3330025751 ISBN 13: 9783330025752
Editorial: LAP LAMBERT Academic Publishing, 2016

Sinopsis

In-vitro embryo production provide an excellent source of embryos for carrying basic research on developmental physiology, farm animal breeding and for commercial application of the emerging biotechniques like embryo sexing, sperm injection, nuclear transfer, cloning and transgenic. The technique of in-vitro maturation, fertilization and culture of embryo essentially involve collection of oocytes from slaughter house ovaries or from living animals through transvaginal aspiration, in-vitro maturation of selected oocytes in media containing serum and hormones, in-vitro fertilization either with frozen thawed semen or freshly collected in-vitro capacitated spermatozoa and in-vitro culture of embryos in media containing oviduct or cumulus cell up to the morulae or blastocyst stage, at which stage they can be either transferred to synchronized recipients or frozen and stored. The present study is aimed to evaluate the effects of different supplementation in the basic media on in-vitro maturation and cleavage rate and to study the developmental competence of in-vitro fertilized goat oocytes on granulosa cell monolayers and oviductal epethelial cells co-culture.

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Reseña del editor

In-vitro embryo production provide an excellent source of embryos for carrying basic research on developmental physiology, farm animal breeding and for commercial application of the emerging biotechniques like embryo sexing, sperm injection, nuclear transfer, cloning and transgenic. The technique of in-vitro maturation, fertilization and culture of embryo essentially involve collection of oocytes from slaughter house ovaries or from living animals through transvaginal aspiration, in-vitro maturation of selected oocytes in media containing serum and hormones, in-vitro fertilization either with frozen thawed semen or freshly collected in-vitro capacitated spermatozoa and in-vitro culture of embryos in media containing oviduct or cumulus cell up to the morulae or blastocyst stage, at which stage they can be either transferred to synchronized recipients or frozen and stored. The present study is aimed to evaluate the effects of different supplementation in the basic media on in-vitro maturation and cleavage rate and to study the developmental competence of in-vitro fertilized goat oocytes on granulosa cell monolayers and oviductal epethelial cells co-culture.

Biografía del autor

Dr. Kaushlendra Pratap Singh born on 20th may, 1976 in Lucknow, is working as Veterinary Medical Officer in Dept. of Animal Husbandry, Uttar Pradesh, India. He obtained his B.V.Sc.& A.H. from G.B.P.U.A.T,Pantnagar in 2003 and M.V.Sc. from Department of Obstetrics and Gynaecology DUVASU, Mathura in 2005. He has published 149 publication in his name.

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Editorial
LAP LAMBERT Academic Publishing
Año de publicación
2016
Idioma
Inglés
ISBN 10 
3330025751
ISBN 13 
9783330025752
Encuadernación
Tapa blanda
Número de páginas
124
Contacto del fabricante
no disponible
Persona responsable
no disponible